Immunohistochemical analysis of estrogen receptors in feline mammary gland benign and malignant lesions: comparison with biochemical assay☆

Mulas, J. Martı́n de las; Niel, M. van; Millán, Y.; Blankenstein, Marinus A.; Mil, F. van

doi:10.1016/s0739-7240(99)00067-3

Cited by 48 publications

(38 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Comparing patterns in mammary carcinomas of zoo felids with those mammary carcinomas arising spontaneously or in progestin-treated domestic cats was not possible because mammary cancer in domestic cats was usually designated by the predominant pattern, and whether multiple patterns occurred was not generally reported. 5,8,13,14,26,28,33,42 Furthermore, tumor classification of mammary gland carcinomas in domestic cats has been inconsistently applied over the years, with tumors often either grouped as adenocarcinomas or subclassified by other patterns.…”

Section: Discussionmentioning

confidence: 99%

Histologic Features of Mammary Carcinomas in Zoo Felids Treated with Melengestrol Acetate (MGA) Contraceptives

2007

View full text Add to dashboard Cite

Abstract. Melengestrol acetate (MGA), a potent synthetic progestin, has been used as a contraceptive in zoo felids since 1975. Mammary gland carcinomas have been linked to MGA treatment in zoo felids, but the histologic features of these tumors and steroid receptor expression have not been described. Zoo felid mammary tumors were requested from participating zoos from 1986 through 1998, and 31 mammary carcinomas from 28 MGA-treated and 3 untreated felids were received. The carcinomas were evaluated on the basis of histologic pattern, tumor grade, and occurrence of metastasis; then features of the tumors were compared to determine if carcinomas in MGA-treated felids differed from those that occur spontaneously. Estrogen-and progesterone-receptor expression was evaluated in 17 of the 31 carcinomas. Of the 31 tumors, 22 (70.9%) had multiple histologic patterns, 29 (93.5%) were high grade, and 28 (90.3%) had metastasized. Within tumors, the tubulopapillary pattern was most common (87.1%, n 5 27); solid (61.3%, n 5 19), cribriform (38.7%, n 5 12), and comedone (25.8%, n 5 8) patterns were less common; and the mucinous (3.2%, n 5 1) pattern was rare. Both MGA-treated and untreated zoo felids had similar patterns and grades of mammary gland cancer as well as prevalence of metastasis. These results indicate that mammary carcinomas in zoo felids are high grade with a predominant tubulopapillary pattern and aggressive behavior. Five of 17 carcinomas expressed progesterone receptors, and 1 of 17 expressed estrogen receptors. Although more zoo felids with cancer had been exposed to MGA in this study, mammary carcinomas were similar in appearance and behavior in untreated and MGA-treated zoo felids. The association of MGA with the development of malignant mammary gland tumors should be considered when using this contraceptive in zoo felids.

show abstract

Section: Discussionmentioning

confidence: 99%

Histologic Features of Mammary Carcinomas in Zoo Felids Treated with Melengestrol Acetate (MGA) Contraceptives

2007

View full text Add to dashboard Cite

show abstract

“…We used also dextran-coated charcoal biochemical assay (DCC) values from the original clinical database as an external standard measure for ER quantitation [26,27]. The data were categorised as negative for 0-1 fmol/mg, weak for 2-9 fmol/mg, moderate for 10-159 fmol/mg, and strong for ‡160 fmol/mg.…”

Section: Tma Construction and Immunostainingmentioning

confidence: 99%

Automated quantitative analysis of estrogen receptor expression in breast carcinoma does not differ from expert pathologist scoring: a tissue microarray study of 3,484 cases

Turbin

Leung

Cheang

et al. 2007

Breast Cancer Res Treat

View full text Add to dashboard Cite

show abstract

“…On the basis of age incidence, histopathology and pattern of metastasis, feline mammary cancer (FMC) could be a good comparative model of human breast cancer (for a review see Weijer et al, 1972). In particular, the lack of estrogen dependency in most tumours (De las Mulas et al, 2000) may indicate that FMC is a suitable animal model for human hormonal-unresponsive breast cancer. The ®nding of over-expression and ampli®cation of cyclin A (Murakami et al, 2000a) and p53 nuclear accumulation (Murakami et al, 2000b) suggests also a similar tumorigenesis.…”

mentioning

confidence: 99%

Feline STK gene expression in mammary carcinomas

et al. 2002

View full text Add to dashboard Cite

The human RON and its mouse homologue stk are members of the MET family of tyrosine kinase receptors. We have previously shown that the RON gene is over-expressed in human breast carcinomas. As cat mammary tumours have been proposed as a suitable model for aggressive human breast cancer, we identi®ed the feline stk gene and studied its expression in cat mammary cancer. Feline stk sequences were found highly homologous to the stk and RON gene exons that encode the juxtamembrane and transmembrane domains of the stk and RON receptors. Feline stk-speci®c transcript was detected by RT ± PCR in cat lung and in 7/8 feline mammary carcinomas and a synchronous skin metastasis examined. Western blot and immunohistochemical analyses were carried out with an antibody that recognized both the human RON and mouse stk receptors. This antibody speci®cally detected a 135 Kd feline protein and stained 10/34 mammary carcinoma archival samples. These data show that the pattern of expression and distribution of the stk protein in feline mammary cancer could be superimposed on that of the RON receptor in human breast cancer and suggest that these feline tumours are a suitable model to test innovative approaches to therapy of aggressive human breast carcinomas. Oncogene (2002) 21, 1785 ± 1790. DOI: 10.1038/sj/ onc/1205221Keywords: tyrosine kinase receptors; mammary carcinomas; companion animals tumours Spontaneous tumours in companion animals oer a unique opportunity as models of human cancer. The relatively high incidence of some cancers, large body size and shorter overall lifespan are factors that contribute to the advantage of these models. Above all, in most cases histopathology and comparable responses to the cytotoxic agents make tumours in companion animals useful to test new therapeutic approaches like new chemotherapeutic regimens, gene therapy and biological modi®cations. However, to date only fragmentary information is available on molecular alterations and biological behaviour of companion animal tumours.In cats, mammary cancer is a frequent neoplasm (%10% all cases, 17% for females), the most common after lympho-hematopoietic and skin tumours. On the basis of age incidence, histopathology and pattern of metastasis, feline mammary cancer (FMC) could be a good comparative model of human breast cancer (for a review see Weijer et al., 1972). In particular, the lack of estrogen dependency in most tumours (De las Mulas et al., 2000) may indicate that FMC is a suitable animal model for human hormonal-unresponsive breast cancer. The ®nding of over-expression and ampli®cation of cyclin A (Murakami et al., 2000a) and p53 nuclear accumulation (Murakami et al., 2000b) suggests also a similar tumorigenesis.Over-expression of tyrosine kinase oncogenes has been reported in many carcinomas (for a review see Kolibaba and Druker, 1997). In vitro and in vivo studies suggested that among these receptors, those of the MET family might be speci®cally involved in the activation of the tyrosine kinase cascade that stimulates invasive properties...

show abstract

Immunohistochemical analysis of estrogen receptors in feline mammary gland benign and malignant lesions: comparison with biochemical assay☆

Cited by 48 publications

References 13 publications

Histologic Features of Mammary Carcinomas in Zoo Felids Treated with Melengestrol Acetate (MGA) Contraceptives

Histologic Features of Mammary Carcinomas in Zoo Felids Treated with Melengestrol Acetate (MGA) Contraceptives

Automated quantitative analysis of estrogen receptor expression in breast carcinoma does not differ from expert pathologist scoring: a tissue microarray study of 3,484 cases

Feline STK gene expression in mammary carcinomas

Contact Info

Product

Resources

About