Immunohistochemical analysis of estrogen and progesterone receptors in endometriosis: comparison with normal endometrium during the menstrual cycle and the effect of medical therapy

Lessey, Bruce A.; Metzger, Déborah; Haney, A.F.; McCarty, Kenneth S.

doi:10.1016/s0015-0282(16)60545-9

Cited by 174 publications

(86 citation statements)

References 18 publications

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“…This pattern of change in the proliferative activity of endometrial epithelial cells during the normal menstrual cycle can be explained by the cyclic regulation of the epithelial expressions of ER and PR (Li et al, 1993;Nisolle et al, 1997). Indeed, it has been reported, and was confirmed here, that the changes in the proliferative activity of the endometrial epithelial cells correlate with the changes in the epithelial expressions of ER and PR in the endometrium, since in the epithelial cells of the functional layer the greatest staining for ER and PR appears in the late proliferative or early secretory phase, and both ER and PR decrease in the mid-to late secretory phases (Scharl et al, 1988;Lessey et al, 1989;Pickartz et al, 1990;Bouchard et al, 1991;Fung et al, 1994;Nisolle et al, 1994Nisolle et al, , 1997Felix et al, 1997).…”

Section: Discussionsupporting

confidence: 84%

Cell kinetic study of the endometrium by nonisotopic in situ hybridization for histone H3 messenger RNA and immunohistochemistry for Ki‐67 and for estrogen and progesterone receptors

et al. 2002

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We investigated the cell kinetics of the endometrium in hysterectomy specimens taken for leiomyoma from 22 women with regular ovulatory menstrual cycles. Formalin-fixed, paraffin-embedded tissue sections were examined for proliferating activity using histone H3 messenger RNA in situ hybridization (H3 mRNA-ISH) and immunostaining for the Ki-67 antigen. The relationship of the proliferative activity of endometrial cells to the immunohistochemical expression of the estrogen receptor (ER) and the progesterone receptor (PR) was also examined. During the menstrual cycle, H3 mRNA expression was observed in both the epithelial cells and the stromal cells of the endometrium. In the functional layer, the labeling indices for H3 mRNA (H3 mRNA-LIs) in the epithelial cells peaked in the late proliferative phase, decreased sharply in the early secretory phase, and remained unchanged thereafter. On the other hand, H3 mRNA-LIs of stromal cells displayed two peaks: one in the midproliferative phase and the other in the late secretory phase, the former peak being the greater. In the basal layer, epithelial cells and stromal cells showed low H3 mRNA-LIs and no significant variation throughout the menstrual cycle. The H3 mRNA-LIs correlated well with the Ki-67-LIs and were lower than the corresponding Ki-67-LIs. The regression coefficient (H3 mRNA-LIs against the Ki-67-LIs) was 0.33 for epithelial cells and 0.49 for stromal cells, suggesting that the cell cycle time was longer for epithelial cells than for stromal cells. The proliferative activity of endometrial cells showed close relationships with the expressions of ER and PR in the endometrium. When used in combination with other proliferative markers in paraffin-embedded tissue sections, H3 mRNA-ISH could open broader perspectives on the cell kinetics of the endometrium. Anat Rec 266: 234 -240, 2002.

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Section: Discussionsupporting

confidence: 84%

Cell kinetic study of the endometrium by nonisotopic in situ hybridization for histone H3 messenger RNA and immunohistochemistry for Ki‐67 and for estrogen and progesterone receptors

et al. 2002

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“…There is no consensus concerning the levels of ER and PR expression in endometriosis, which were variable in different studies, either higher than in endometrium (35)(36) or lower (37)(38). The differences between the studies could be explained by the site of endometriotic biopsy (ovarian vs. peritoneal) or by the assay methods used.…”

Section: Discussionmentioning

confidence: 99%

Reduction of apoptosis and proliferation in endometriosis

Béliard

Noël

Foidart

2004

Fertility and Sterility

151

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Objective: To evaluate whether endometriosis could be related to an impaired balance between apoptosis and proliferation, two processes which could be modulated by hormonal status.Design: Immunohistochemical study.Setting: Academic research laboratory. Intervention(s):Endometriotic samples obtained from peritoneum of women aged 26-40 years who were undergoing laparoscopy for pain or infertility. Main Outcome Measure(s):Apoptotic cells were detected with the use of the terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assay. The production of p53 and bcl-2, estrogen and Progesterone (P) receptors, and cellular proliferation were assessed by immunohistochemistry in eutopic and ectopic endometria from 30 patients with endometriosis throughout the menstrual cycle. Results were compared with those from normal endometria from 15 fertile patients. Result(s):Endometriotic lesions were characterized by reduced TUNEL and p53 stainings and by enhanced bcl-2 staining. No correlation between apoptosis and estrogen receptor or P receptor levels was found. A lower amount of steroid receptor was found in endometriotic tissues, without cyclic modulation, compared with the eutopic endometrium. Conclusion(s):Our results suggest that when endometrial tissue is located at ectopic locations, it differs from eutopic endometrium by its proliferation rate, steroid hormone levels, and markers of apoptosis. A reduced sensitivity of endometriotic cells to apoptosis could promote the dissemination and implantation of these cells to ectopic sites.

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“…Endometrial implants contain estrogen, progesterone, and androgen receptors (Lessey et al 1989;Prentice et al 1992;Bergqvist and Fernö 1993), as well as aromatase, an enzyme that catalyses the conversion of androgens to estrogens. Ectopic endometriotic implants respond to ovarian hormonal changes.…”

Section: Introductionmentioning

confidence: 99%

Polymorphisms and haplotypes of the gene encoding the estrogen-metabolizing CYP19 gene in Korean women: no association with advanced-stage endometriosis

Hur

Lee

et al. 2007

J Hum Genet

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A variety of factors affect the development of endometriosis, including hormonal status and genetic factors. The growth of endometriosis is stimulated by local estrogen production in conjunction with circulating estrogen. The CYP19 gene encodes a steroid aromatase that catalyses the conversion of C-19 androgens to estrogens. This study investigated whether polymorphisms of the CYP19 gene are associated with the risk of advanced endometriosis in Korean women. Blood samples were collected from 224 female patients with endometriosis of stages III and IV, as diagnosed by both pathologic and laparoscopic findings, and from a control group comprising of 188 women undergoing laparoscopic surgery or laparotomy for nonmalignant lesions. Single-nucleotide polymorphisms, restriction fragment length polymorphisms, and tetranucleotide tandem repeat polymorphisms were discriminated by the polymerase chain reaction (PCR). Haplotype analysis was also performed. CYP19 115T>C, 240G>A, and 1531C>T polymorphisms and [TTTA]n tetranucleotide repeat polymorphisms in the CYP19 gene and their haplotypes were not significantly associated with the risk of endometriosis. The risk of endometriosis also did not increase significantly with the number of higher risk alleles of the CYP19 gene. In conclusion, our findings suggest that CYP19 genetic polymorphisms are not associated with advanced-stage endometriosis in Korean women.

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Immunohistochemical analysis of estrogen and progesterone receptors in endometriosis: comparison with normal endometrium during the menstrual cycle and the effect of medical therapy

Cited by 174 publications

References 18 publications

Cell kinetic study of the endometrium by nonisotopic in situ hybridization for histone H3 messenger RNA and immunohistochemistry for Ki‐67 and for estrogen and progesterone receptors

Cell kinetic study of the endometrium by nonisotopic in situ hybridization for histone H3 messenger RNA and immunohistochemistry for Ki‐67 and for estrogen and progesterone receptors

Reduction of apoptosis and proliferation in endometriosis

Polymorphisms and haplotypes of the gene encoding the estrogen-metabolizing CYP19 gene in Korean women: no association with advanced-stage endometriosis

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