Immunohistochemical analysis of cellular adhesion molecules (ICAM-1, VCAM-1) and VEGF in fibrovascular membranes of patients with proliferative diabetic retinopathy: Preliminary study

Khalfaoui, Taoufik; Lizard, Gérard; Beltaief, O.; Colin, Didier; Hamida, Jeannette Ben; Errais, K.; Ammous, I.; Zbiba, W.; Tounsi, L.; Zhioua, R.; Anane, R.; Ouertani-Meddeb, A.

doi:10.1016/j.patbio.2008.07.021

Cited by 19 publications

(13 citation statements)

References 22 publications

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“…CAMs are proteins located on cell surface; the binding of CAMs to their receptors is important in the mediation of inflammatory and immune reactions (Golias et al, 2007). Previous studies have suggested that CAMs are important in the development of DR (Khalfaoui et al, 2009;Ugurlu et al, 2013 of insulin and is associated with DR neovascularization (Qin, Zhang, & Xu, 2015;Sasore, Reynolds, & Kennedy, 2014). Compared with the results of transcriptomic analysis which use CD31+ vascular endothelial cells obtained from human PDR fibrovascular membranes (FVM) (Lam et al, 2017), we found the gene Col3a1 were both significantly changed, and according to the GO enrichment analysis of two researches, Col3a1 may belong to blood vessel development term (GO:0001568), suggested that Col3a1 may play a role in DR neovascularization.…”

Section: Discussionmentioning

confidence: 97%

Identification of novel differentially expressed genes in retinas of STZ‐induced long‐term diabetic rats through RNA sequencing

Xing

Jiang

Zhang

et al. 2020

Molec Gen & Gen Med

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Background The aim of this research was to investigate the retinal transcriptome changes in long‐term streptozotocin (STZ)‐induced rats' retinas using RNA sequencing (RNA‐seq), to explore the molecular mechanisms of diabetic retinopathy (DR), and to identify novel targets for the treatment of DR by comparing the gene expression profile we obtained. Methods In this study, 6 healthy male SD rats were randomly divided into wild‐type (WT) group and streptozotocin (STZ)‐induced group, 3 rats each group. After 6 months, 3 normal retina samples and 3 DM retina samples (2 retinas from the same rat were considered as 1 sample) were tested and differentially expressed genes (DEGs) were measured by RNA‐seq technology. Then, we did Gene Ontology (GO) enrichment analysis and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway analysis and validated the results of RNA‐seq through qRT–PCR. Results A total of 118 DEGs were identified, of which 72 were up‐regulated and 46 were down‐regulated. The enriched GO terms showed that 3 most significant enrichment terms were binding (molecular function), cell part (cellular component), and biological regulation (biological process). The results of the KEGG pathway analysis revealed a significant enrichment in cell adhesion molecules, PI3K‐Akt signaling pathway, and allograft rejection, etc. Conclusion Our research has identified specific DEGs and also speculated their potential functions, which will provide novel targets to explore the molecular mechanisms of DR.

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Section: Discussionmentioning

confidence: 97%

Identification of novel differentially expressed genes in retinas of STZ‐induced long‐term diabetic rats through RNA sequencing

Xing

Jiang

Zhang

et al. 2020

Molec Gen & Gen Med

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“…Interestingly, no CD11a (LFA-1) positivity could be observed in both cell types under basal conditions. The cellular and soluble forms of ICAM-1 have been frequently detected in diabetic fibrovascular membranes [47], epiretinal membranes, serum or vitreous [48–51] of patients with PDR. Although CD146 has been known to be expressed on many cell types under physiological or pathological conditions, its presence and function in fvERM or primary hRPE cells has not yet been described.…”

Section: Discussionmentioning

confidence: 99%

Functional and Molecular Characterization ofEx VivoCultured Epiretinal Membrane Cells from Human Proliferative Diabetic Retinopathy

Veréb

Lumi

Andjelić

et al. 2013

BioMed Research International

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Characterization of the cell surface marker phenotype of ex vivo cultured cells growing out of human fibrovascular epiretinal membranes (fvERMs) from proliferative diabetic retinopathy (PDR) can give insight into their function in immunity, angiogenesis, and retinal detachment. FvERMs from uneventful vitrectomies due to PDR were cultured adherently ex vivo. Surface marker analysis, release of immunity- and angiogenesis-pathway-related factors upon TNFα activation and measurement of the intracellular calcium dynamics upon mechano-stimulation using fluorescent dye Fura-2 were all performed. FvERMs formed proliferating cell monolayers when cultured ex vivo, which were negative for endothelial cell markers (CD31, VEGFR2), partially positive for hematopoietic- (CD34, CD47) and mesenchymal stem cell markers (CD73, CD90/Thy-1, and PDGFRβ), and negative for CD105. CD146/MCAM and CD166/ALCAM, previously unreported in cells from fvERMs, were also expressed. Secretion of 11 angiogenesis-related factors (DPPIV/CD26, EG-VEGF/PK1, ET-1, IGFBP-2 and 3, IL-8/CXCL8, MCP-1/CCL2, MMP-9, PTX3/TSG-14, Serpin E1/PAI-1, Serpin F1/PEDF, TIMP-1, and TSP-1) were detected upon TNFα activation of fvERM cells. Mechano-stimulation of these cells induced intracellular calcium propagation representing functional viability and role of these cells in tractional retinal detachment, thus serving as a model for studying tractional forces present in fvERMs in PDR ex vivo.

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“…The substantial overexpression of VEGF was demonstrated in fibrovascular membranes in patients with proliferative diabetic retinopathy, suggesting that this molecule might contribute to the development of PDR [40]. Expression of growth factors, such as VEGF, basic fibroblast growth factor (BFGF), and insulin-like growth factor (IGF), is influenced by single-nucleotide polymorphisms (SNPs) in these genes [41–52].…”

Section: Gene Polymorphisms Of Growth Factorsmentioning

confidence: 99%

“…The substantial overexpression of adhesion molecules ICAM-1, VCAM-1, and of VEGF was demonstrated in fibrovascular membranes in patients with proliferative diabetic retinopathy, suggesting that these molecules might contribute to the development of PDR [40]. Few studies have so far tested possible association between risk genotypes of adhesion molecules and PDR in type 2 diabetes (Table 6) [18, 64].…”

Section: Gene Polymorphisms Of the Adhesion Molecules And Pdrmentioning

confidence: 99%

Candidate Genes for Proliferative Diabetic Retinopathy

Petrovič¹

2013

BioMed Research International

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Several candidate genes have been so far implicated in the pathogenesis of proliferative diabetic retinopathy (PDR) in subjects with type 2 diabetes. Since the principal pathogenetic mechanisms for diabetic retinopathy (DR) and PDR are different, the main pathogenetic mechanism in DR is increased vascular permeability, whereas in PDR the crucial pathogenetic mechanisms are fibrosis and neoangiogenesis. Due to that fact, different candidate genes are expected to be involved in the development of either DR or PDR. None of the candidate genes, however, can be fully and solely responsible for the development of PDR and for DR progression into PDR. Epigenetic mechanisms are expected to be involved in the pathogenesis of PDR as well. Gene polymorphisms responsible for PDR and epigenetic mechanisms responsible for PDR are reviewed in this paper.

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Immunohistochemical analysis of cellular adhesion molecules (ICAM-1, VCAM-1) and VEGF in fibrovascular membranes of patients with proliferative diabetic retinopathy: Preliminary study

Cited by 19 publications

References 22 publications

Identification of novel differentially expressed genes in retinas of STZ‐induced long‐term diabetic rats through RNA sequencing

Identification of novel differentially expressed genes in retinas of STZ‐induced long‐term diabetic rats through RNA sequencing

Functional and Molecular Characterization ofEx VivoCultured Epiretinal Membrane Cells from Human Proliferative Diabetic Retinopathy

Candidate Genes for Proliferative Diabetic Retinopathy

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