Immunohistochemical Alterations in Basement Membrane Components of Squamous Cell Carcinoma

Oguro, K.; Kazama, Tatsuo; Isemura, Mamoru; Nakamura, Takamichi; Akai, Shigeyasu; Satō, Yoshio

doi:10.1111/1523-1747.ep12462197

Cited by 28 publications

(16 citation statements)

References 17 publications

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“…Morpheaform tumors are thought to represent a more locally invasive form of BCC, and they have a number of biological features that separate them from their nodular counterparts. Several previous studies of BCC 1–3 have found differences in the expression of basement membrane components based on histologic subtype. Nodular tumors express certain components constantly, while infiltrative subtypes show only intermittent or absent expression.…”

Section: Commentarymentioning

confidence: 85%

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Abnormal Expression of Epiligrin and α6β4 Integrin in Basal Cell Carcinoma

Schofield

Kist²,

Lucas³

et al. 1998

Dermatologic Surgery

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Section: Commentarymentioning

confidence: 85%

“…Extensive investigations using immunohistochemistry have been performed examining the normal components of the epidermal BM surrounding BCC. Laminin‐1, 1–3 laminin‐5 3 (nicein), types IV 1–3 and VII 3 collagens, integrin subchains, 3–5 and bullous pemphigoid(BP) Ag 1 have shown variable expression in vivo compared with normal skin.…”

mentioning

confidence: 99%

Abnormal Expression of Epiligrin and α6β4 Integrin in Basal Cell Carcinoma

Schofield

Kist²,

Lucas³

et al. 1998

Dermatologic Surgery

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show abstract

“…In patients, cSCC often does not affect the normal E-cadherin expression [19], but collagen-IV degradation concurs with lost tissue polarity, tumor cell invasion, and metastasis [25]. Collagen-IV fragments surround the carcinoma nests of cSCC patients [26] too. For the most part, the cSCC-E model emulated the morphology of a carcinoma in situ with characteristic epidermal foci of cells with atypical, enlarged, irregular, and hyperchromatic nuclei (Fig.…”

Section: Discussionmentioning

confidence: 99%

The barrier function of organotypic non-melanoma skin cancer models

Zoschke¹,

Ulrich

Sochorová

et al. 2016

Journal of Controlled Release

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“…These differences have been demonstrated to be correlated with the differentiation status and the degree of keratinization of primary SCC. In well‐differentiated and highly keratinized SCC, laminin 332 and collagen type IV were strongly expressed in the tumor nest near the BM without loss of continuity (30). In poorly differentiated and poorly keratinized SCC, these proteins were largely lost, suggesting that these SCC cause greater enzymatic degradation of BM components than well‐differentiated SCC.…”

Section: Discussionmentioning

confidence: 99%

An in vitro three‐dimensional model of primary human cutaneous squamous cell carcinoma

Commandeur

Gruijl

Willemze

et al. 2009

Experimental Dermatology

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Squamous cell carcinomas (SCC) represent a substantial clinical problem because of increases, frequent recurrences and successive de novo tumors, especially in organ transplant recipients. To improve upon the current surgical and other non-selective therapies, a validated organotypic in vitro model of primary human SCC needs to be developed. Such a model will have obvious advantages over current cell line and animal based approaches, and may render the latter partly obsolete. In a first approach, an explant technique of primary SCC biopsies onto dermal constructs was used to emulate tumor expansion in an in vitro model. Histological analysis revealed the formation of nests of squamous cells, mimicking an invasive morphological feature of primary SCC. Immunohistochemical analysis comprised an array of markers characteristic of keratinocyte (hyper) proliferation (K6, K16, K17 and Ki67), differentiation (K1, K10 and involucrin), basement membrane (collagen types IV and VII, integrins alpha(6) and beta(4) and laminin 332) and SCC (K4, K13 and Axl). The generated human SCC models displayed disturbed differentiation and keratins associated with hyperproliferation, but a low frequency of Ki67 positive cells. Basement membrane composition of the in vitro SCC model resembled that of normal skin. These results show for the first time that in vitro modelling of three-dimensional growth of primary cutaneous human SCC is feasible. This model may provide a platform to develop refined preventive and curative treatments and thereby gain understanding of SCC pathogenesis.

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Immunohistochemical Alterations in Basement Membrane Components of Squamous Cell Carcinoma

Cited by 28 publications

References 17 publications

Abnormal Expression of Epiligrin and α6β4 Integrin in Basal Cell Carcinoma

Abnormal Expression of Epiligrin and α6β4 Integrin in Basal Cell Carcinoma

The barrier function of organotypic non-melanoma skin cancer models

An in vitro three‐dimensional model of primary human cutaneous squamous cell carcinoma

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