IMMUNOHEMATOLOGY: Novel antibody screening cells, MUT+Mur kodecytes, created by attaching peptides onto red blood cells

Abstract

This study used selected MUT and Mur peptides and KODE cell surface engineering technology to create MUT+Mur kodecytes suitable for the detection and identification of RBC antibodies in human serum or plasma. This technology has the potential to create a large range of specialized RBCs for antibody screening and identification.

FSL Constructs: A Simple Method for Modifying Cell/Virion Surfaces with a Range of Biological Markers Without Affecting their Viability

“…2. FSL constructs can be used to label the outside of a variety of live cells [3][4][5][6][7][8][9]11,14 . In the examples shown in Figure 2 embryos at different stages were used (as they are a fragile cell), but other cells (Figure 3) or enveloped virions (Figure 4) can also be labeled.…”

“…FSL construct functional moieties (F) so far include a range of saccharides including blood group-related determinants, sialic acids, hyaluronan polysaccharides, fluorophores, biotin, radiolabels, and a range of peptides [3][4][5][6][7][8][9][10][11][12] . FSL constructs have been used in modifying embryos, spermatozoa, zebrafish, epithelial/endometrial cells, red blood cells, and virions to create quality controls systems and diagnostic panels, to modify cell adhesion/ interaction/ separation/ immobilization, and for in vitro and in vivo imaging of cells/virions [3][4][5][6][7][8][9][10][11][12] .…”

“…Washing is optional. Cells modified by FSL constructs are known as kodecytes [6][7][8][9] , while virions are kodevirions 10 . FSL constructs as direct infusions and kodecytes/kodevirions have been used in experimental animal models 7,8,10 .…”

“…FSL constructs have been used in modifying embryos, spermatozoa, zebrafish, epithelial/endometrial cells, red blood cells, and virions to create quality controls systems and diagnostic panels, to modify cell adhesion/ interaction/ separation/ immobilization, and for in vitro and in vivo imaging of cells/virions [3][4][5][6][7][8][9][10][11][12] .…”

“…The most common procedure is incubation of cells (in lipid free media) with a solution for FSL constructs for 1-2 hours at 37°C [4][5][6][7][8][9][10] . During the incubation the FSL constructs spontaneously incorporate into the membrane, and the process is complete.…”