Immunogold method evidences that kinesin and myosin bind to and couple microtubules and actin filaments in lipotubuloids of Ornithogalum umbellatum ovary epidermis

Kwiatkowska, Maria; Polit, Justyna Teresa; Popłońska, Katarzyna; Stępiński, Dariusz; Wojtczak, Agnieszka

doi:10.1007/s11738-013-1235-8

Cited by 6 publications

(7 citation statements)

References 72 publications

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“…The fact that lipotubuloids are able to move in a cell together with the cytoplasm streaming (progressive movement) as well as dynamically, autonomously rotate with a speed sixfold greater than that of cyclosis (Kwiatkowska 1972b ; Kwiatkowska et al 2009 ), undoubtedly facilitates cutinsome release from lipotubuloids and their subsequent transport to a cell wall. Our latest immunogold studies seemed to show that the lipotubuloid’s ability to rotate autonomously resulted from the fact that kinesin and myosin formed the link between microtubules and actin filaments in lipotubuloids (Kwiatkowska et al 2013 ).…”

Section: Discussionmentioning

confidence: 99%

Cutinsomes and lipotubuloids appear to participate in cuticle formation in Ornithogalum umbellatum ovary epidermis: EM–immunogold research

et al. 2014

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The outer wall of Ornithogalum umbellatum ovary and the fruit epidermis are covered with a thick cuticle and contain lipotubuloids incorporating 3H-palmitic acid. This was earlier evidenced by selective autoradiographic labelling of lipotubuloids. After post-incubation in a non-radioactive medium, some marked particles insoluble in organic solvents (similar to cutin matrix) moved to the cuticular layer. Hence, it was hypothesised that lipotubuloids participated in cuticle synthesis. It was previously suggested that cutinsomes, nanoparticles containing polyhydroxy fatty acids, formed the cuticle. Thus, identification of the cutinsomes in O. umbellatum ovary epidermal cells, including lipotubuloids, was undertaken in order to verify the idea of lipotubuloid participation in cuticle synthesis in this species. Electron microscopy and immunogold method with the antibodies recognizing cutinsomes were used to identify these structures. They were mostly found in the outer cell wall, the cuticular layer and the cuticle proper. A lower but still significant degree of labelling was also observed in lipotubuloids, cytoplasm and near plasmalemma of epidermal cells. It seems that cutinsomes are formed in lipotubuloids and then they leave them and move towards the cuticle in epidermal cells of O. umbellatum ovary. Thus, we suggest that (1) cutinsomes could take part in the synthesis of cuticle components also in plant species other than tomato, (2) the lipotubuloids are the cytoplasmic domains connected with cuticle formation and (3) this process proceeds via cutinsomes.

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Section: Discussionmentioning

confidence: 99%

Cutinsomes and lipotubuloids appear to participate in cuticle formation in Ornithogalum umbellatum ovary epidermis: EM–immunogold research

et al. 2014

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“…Sections were treated with 10% hydrogen peroxide for 15 min to remove osmium and washed in distilled water and in phosphatebuffered saline (PBS) buffer (0.01 M, pH 7.4) (Kwiatkowska et al, 2013). Samples were then blocked with small drops of PBS buffer containing 3% bovine serum albumin for 1 h at room temperature and later washed three times in PBS buffer.…”

Section: Tissue Sectioning and Immunolabeling For Temmentioning

confidence: 99%

Ultrastructure of the Epidermal Cell Wall and Cuticle of Tomato Fruit (Solanum lycopersicum L.) during Development

2015

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The epidermis plays a pivotal role in plant development and interaction with the environment. However, it is still poorly understood, especially its outer epidermal wall: a singular wall covered by a cuticle. Changes in the cuticle and cell wall structures are important to fully understand their functions. In this work, an ultrastructure and immunocytochemical approach was taken to identify changes in the cuticle and the main components of the epidermal cell wall during tomato fruit development. A thin and uniform procuticle was already present before fruit set. During cell division, the inner side of the procuticle showed a globular structure with vesicle-like particles in the cell wall close to the cuticle. Transition between cell division and elongation was accompanied by a dramatic increase in cuticle thickness, which represented more than half of the outer epidermal wall, and the lamellate arrangement of the non-cutinized cell wall. Changes in this non-cutinized outer wall during development showed specific features not shared with other cell walls. The coordinated nature of the changes observed in the cuticle and the epidermal cell wall indicate a deep interaction between these two supramolecular structures. Hence, the cuticle should be interpreted within the context of the outer epidermal wall.

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“…They are rich in ribosomes forming numerous polysomes and rough endoplasmic reticulum (RER). Moreover, they contain numerous lipid bodies entwined with microtubules, which in turn are connected by myosin and kinesin to actin filaments (Kwiatkowska et al, 2013). In LMs, there are also very few mitochondria, Golgi structures, or microbodies (peroxisomes and glyoxysomes).…”

Section: Structure and Function Of Lipotubuloids As Lmsmentioning

confidence: 99%

Lipotubuloids in ovary epidermis of Ornithogalum umbellatum act as metabolons: suggestion of the name ‘lipotubuloid metabolon’

Kwiatkowska

Polit

Stępiński

et al. 2014

Journal of Experimental Botany

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A metabolon is a temporary, structural-functional complex formed between sequential metabolic enzymes and cellular elements. Cytoplasmic domains called lipotubuloids are present in Ornithogalum umbellatum ovary epidermis. They consist of numerous lipid bodies entwined with microtubules, polysomes, rough endoplasmic reticulum (RER), and actin filaments connected to microtubules through myosin and kinesin. A few mitochondria, Golgi structures, and microbodies are also observed and also, at later development stages, autolytic vacuoles. Each lipotubuloid is surrounded by a tonoplast as it invaginates into a vacuole. These structures appear in young cells, which grow intensively reaching 30-fold enlargement but do not divide. They also become larger due to an increasing number of lipid bodies formed in the RER by the accumulation of lipids between leaflets of the phospholipid bilayer. When a cell ceases to grow, the lipotubuloids disintegrate into individual structures. Light and electron microscope studies using filming techniques, autoradiography with [(3)H]palmitic acid, immunogold labelling with antibodies against DGAT2, phospholipase D1 and lipase, and double immunogold labelling with antibodies against myosin and kinesin, as well as experiments with propyzamide, a microtubule activity inhibitor, have shown that lipotubuloids are functionally and structurally integrated metabolons [here termed lipotubuloid metabolons (LMs)] occurring temporarily in growing cells. They synthesize lipids in lipid bodies in cooperation with microtubules. Some of these lipids are metabolized and used by the cell as nutrients, and others are transformed into cuticle whose formation is mediated by cutinsomes. The latter were discovered in planta using specific anti-cutinsome antibodies visualized by gold labelling. Moreover, LMs are able to rotate autonomously due to the interaction of microtubules, actin filaments, and motor proteins, which influence microtubules by changing their diameter.

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Immunogold method evidences that kinesin and myosin bind to and couple microtubules and actin filaments in lipotubuloids of Ornithogalum umbellatum ovary epidermis

Cited by 6 publications

References 72 publications

Cutinsomes and lipotubuloids appear to participate in cuticle formation in Ornithogalum umbellatum ovary epidermis: EM–immunogold research

Cutinsomes and lipotubuloids appear to participate in cuticle formation in Ornithogalum umbellatum ovary epidermis: EM–immunogold research

Ultrastructure of the Epidermal Cell Wall and Cuticle of Tomato Fruit (Solanum lycopersicum L.) during Development

Lipotubuloids in ovary epidermis of Ornithogalum umbellatum act as metabolons: suggestion of the name ‘lipotubuloid metabolon’

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