Immunoglobulins in human dental plaque

Newman, H. N.; Seymour, G. J.; Challacombe, Stephen

doi:10.1111/j.1600-0765.1979.tb00212.x

Cited by 18 publications

(9 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Salivary activity that causes agglutination can influence plaque formation. 12,20,21 Salivary agglutinating activity can cause Aa to form large aggregates. 12 This agglutination process could facilitate removal and swallowing of Aa thus reducing its ability be retained in the oral cavity.…”

Section: Discussionmentioning

confidence: 99%

Can salivary activity predict periodontal breakdown in A. actinomycetemcomitans infected adolescents?

Fine

Furgang

McKiernan

et al. 2013

Archives of Oral Biology

View full text Add to dashboard Cite

Objective While Aggregatibacter actinomycetemcomitans (Aa) is highly associated with localized aggressive periodontitis (LAP) many Aa-carriers do not develop LAP. This study was designed to determine whether specific salivary factors could distinguish between subjects who have Aa initially and remain healthy (H/AA) as compared to those who develop LAP (LAP/AA). Design H/AA subjects and healthy controls with no Aa (H) were enrolled in a longitudinal cohort study to investigate initiation of bone loss (LAP) over 3 years. After detection of LAP, stored saliva from 10 H, 10 H/AA, and 10 LAP/AA subjects was thawed, processed, and tested for; 1) lactoferrin (Lf) concentration and iron levels, 2) agglutination of Aa; 3) killing of Gram-positive bacteria. Results LAP/AA saliva levels of Lf iron were low prior to and after bone loss (3.6 ± 1.7 ng Fe/µg (LAP/AA vs. H and H/AA p ≤ 0.01). Saliva from H/AA subjects caused Aa to agglutinate significantly more than H or LAP/AA saliva (p ≤ 0.01). LAP/AA saliva killed Streptococcus mutans, S. sanguis and Lactobacillus in vitro by > 83%. Saliva from H individuals killed these bacteria by < 3.3% (LAP/AA vs. H; p ≤ 0.01). H/AA killing was intermediate. Conclusions LAP/AA saliva showed: low levels of Lf iron, minimal Aa agglutinating activity, and high killing activity against Gram-positive bacteria. Aa-positive healthy saliva (H/AA) showed: higher levels of Lf iron, maximal Aa agglutinating activity, and moderate killing of Gram-positive bacteria. A salivary activity profile can distinguish between subjects who are Aa-positive and remain healthy from those who develop LAP.

show abstract

Section: Discussionmentioning

confidence: 99%

Can salivary activity predict periodontal breakdown in A. actinomycetemcomitans infected adolescents?

Fine

Furgang

McKiernan

et al. 2013

Archives of Oral Biology

View full text Add to dashboard Cite

show abstract

“…Antibodies to S. mutans have been detected in hu man serum [Challacombe, 1974] and these can reach the gingival crevice via the crevicular fluid [Challa combe et al, 1978], Serum Ig has been demonstrated by IF staining in the apical plaque border on approxi mal tooth surfaces [Newman et al, 1979]. Emilson et al [1974] suggested that such host Ig could affect the reliability of the IF technique if it masked bacterial antigens.…”

Section: Discussionmentioning

confidence: 99%

A Method for the Identification of Streptococcus mutans in Gingival Margin Plaque by Immunofluorescence

Bush

Challacombe

Newman³

1990

Caries Res

View full text Add to dashboard Cite

A method was developed to identify Streptococcus mutans in natural dental plaque by indirect immunofluorescence staining, using a high-titred polyclonal antiserum raised against a serotype c strain of S. mutans followed by an FITC conjugate. Specificity was determined by staining 45 representative strains of plaque organisms, which demonstrated minimal cross-reactions. In vitro incubation of S. mutans NCTC 10449 films with a human serum containing antibodies to S. mutans and the presence of extracellular polysaccharide did not inhibit staining. The staining method enabled 98% of the streptococci to be detected in mixtures of S. mutans NCTC 10449 and Lactobacillus casei NCTC 10302. S. mutans was detected at a ratio of 1:100,000 in mixtures of pure cultures. In plaque samples, S. mutans could be distinguished from other organisms, including an unidentified cross-reacting bacillus found in some gingival plaque samples. The results suggest that immunofluorescence is a fast, practical method for identifying specific bacteria in plaque and, therefore, could be of use in microbiological studies of caries.

show abstract

“…This must be regarded as highly probable in view of the fact that everything stained all of the time. More recently, Newman et al (1979) have demonstrated the in situ presence of immunoglobuhns in plaque. However, whether or not these immunoglobulins represent specific anti-plaque antibodies remains to be demonstrated.…”

Section: \ Humoral Aspectsmentioning

confidence: 99%

The immunopathogenesis of progressive chronic inflammatory periodontal disease

Seymour

Powell

Davies

1979

J Oral Pathology Medicine

View full text Add to dashboard Cite

Abstract. Natural, humoral and cellular immune mechanisms have all been implicated in the pathogenesis of chronic inflammatory periodontal disease. However, confusion still exists as to the role played by each of these immunological mechanisms. Recently, characterization of the cell types within the progressive lesion has been established, in which four recognizable zones were described. Immediately subjacent to the epithelium lining the periodontal pocket both polymorphonuclear leukocytes (PMN's) and macrophages were seen, while cells deeper in the tissues had the morphological appearance of lymphocytes. The majority of these lymphocytes had a B‐cell phenotype although a few T‐cells and macrophages were found. On the advancing front of the lesion the cells had the morphological appearance of plasma cells, the majority of which contained IgG. Other cells found in this region had the morphology of plasma cells yet contained no cytoplasmic immunoglobulin, but they did contain substantial amounts of lysosomal enzymes. Similar cells have previously been described in periodontal disease; their frequent association with fibroblasts may suggest that they are important in the pathogenesis. Deposits of IgG and fibrin were found in the fibrous tissue band surrounding the lesion. These results are reviewed and, although the zones described were not anatomically distinct, by describing the lesion in this way it was possible to establish a convenient model to explain the immunopathogenesis of progressive chronic inflammatory periodontal disease. In this respect, progressive chronic inflammatory periodontal disease in man should be considered as a B‐cell lesion.

show abstract

Immunoglobulins in human dental plaque

Cited by 18 publications

References 29 publications

Can salivary activity predict periodontal breakdown in A. actinomycetemcomitans infected adolescents?

Can salivary activity predict periodontal breakdown in A. actinomycetemcomitans infected adolescents?

A Method for the Identification of Streptococcus mutans in Gingival Margin Plaque by Immunofluorescence

The immunopathogenesis of progressive chronic inflammatory periodontal disease

Contact Info

Product

Resources

About