Immunoglobulin Variable Region Genes

Cory, Suzanne

doi:10.1159/000156923

Cited by 2 publications

(1 citation statement)

References 35 publications

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“…Nucleotides above the A8.1 sequence denote differences with the VHIOI sequence and open arrow heads mark positions where the indicated extra nucleotides appear in the germline VHIOI sequence. the VHA8 gene is also related to those expressed in HOPC-1 and M141 (see Cory, 1984).…”

Section: Resultsmentioning

confidence: 99%

Activation of immunoglobulin mu gene expression involves stepwise demethylation.

Gerondakis¹,

Boyd²,

Bernard³

et al. 1984

The EMBO Journal

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In an attempt to identify stages of mu gene activation subsequent to VHDHJH assembly, we investigated two pre‐B cell lines (A1 and A8) that have both alleles of the JH locus rearranged but do not make mu polypeptides. The block in A1 reflects incorrect VHDHJH assembly on both alleles. In A8, although an out‐of‐phase VHDHJH‐C mu allele is transcribed, the properly assembled allele is silent, despite having a normal VH promoter and mu enhancer. Thus, transcription can be restricted to a single mu allele. Low level mu transcription in both lines was associated with demethylation of the VHDHJH and enhancer regions but not of the C mu gene. Markedly elevated mu transcription ensued on lipopolysaccharide stimulation or fusion to a plasmacytoma, but only fusion induced C mu demethylation. Hence stepwise demethylation is implicated in mu gene regulation, but enhanced expression can also occur independently of, or prior to, demethylation.

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Section: Resultsmentioning

confidence: 99%