Immunoglobulin secretion by isolated intestinal lymphocytes: spontaneous production and T-cell regulation in normal small intestine and in patients with coeliac disease.

Crabtree, J. E.; Heatley, R. V.; Losowsky, M L

doi:10.1136/gut.30.3.347

Cited by 21 publications

(8 citation statements)

References 20 publications

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“…It is known that a strong humoral immune response occurs in untreated CD, which is predominantly of IgA and IgG classes in serum (systemic immunity) and IgA and IgM classes in jejunal aspirate and gut lavage fluid (mucosal immunity). Enhanced local secretion of IgA (p \ 0.05) and IgM (p \ 0.001) with respect to controls has also been demonstrated in CD patients using in vitro lymphocyte culture [41]. Our data confirm the concept of IgM segregation in the gut, and indicate that IgMs and B cells are important for CD immunopathogenesis [30,31].…”

Section: Differential In Gel Protein Expression In CD Patients and Cosupporting

confidence: 88%

“…IgM secretion has previously been reported to be associated with CD [41]; as IgM antibodies can activate complement, it is suggested that they might contribute to the damage following an encounter with antigens (e.g., gluten) [42,43]. It is known that a strong humoral immune response occurs in untreated CD, which is predominantly of IgA and IgG classes in serum (systemic immunity) and IgA and IgM classes in jejunal aspirate and gut lavage fluid (mucosal immunity).…”

Section: Differential In Gel Protein Expression In CD Patients and Comentioning

confidence: 98%

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Proteomic Analyses Lead to a Better Understanding of Celiac Disease: Focus on Epitope Recognition and Autoantibodies

Simula

Canzonieri

et al. 2010

Dig Dis Sci

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Proteomic technologies are being used with increasing frequency in the scientific community. In this review, we have highlighted their use in celiac disease (CD). The available techniques, which include two-dimensional (2D) gel electrophoresis, mass spectrometry, and antibody and tissue arrays, have been used to identify proteins or changes in protein expression specific to gut tissue from patients with CD. A number of studies have employed proteomic methodologies to determine the diagnostic biomarkers in body fluids or to examine changes in protein expression and posttranslational modifications during signaling. A fast technological development of these methods, along with the combination of classic techniques with proteomics, will lead to new discoveries, which will consent a better understanding of the pathogenesis of CD.

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Section: Differential In Gel Protein Expression In CD Patients and Cosupporting

confidence: 88%

Section: Differential In Gel Protein Expression In CD Patients and Comentioning

confidence: 98%

Proteomic Analyses Lead to a Better Understanding of Celiac Disease: Focus on Epitope Recognition and Autoantibodies

Simula

Canzonieri

et al. 2010

Dig Dis Sci

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“…An enhanced local IgM secretion had already been demonstrated in CD patients (37). Since IgM antibodies can activate complement, it has been suggested that they might contribute to the damage following the encounter with antigens (for example, gluten) (38,39).…”

Section: Discussionmentioning

confidence: 99%

PPAR Signaling Pathway and Cancer-Related Proteins Are Involved in Celiac Disease-Associated Tissue Damage

Simula

Cannizzaro

Canzonieri

et al. 2010

Mol Med

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Celiac disease (CD) is an immune-mediated disorder triggered by the ingestion of wheat gliadin and related proteins in genetically predisposed individuals. To find a proteomic CD diagnostic signature and to gain a better understanding of pathogenetic mechanisms associated with CD, we analyzed the intestinal mucosa proteome alterations using two dimensional difference gel electrophoresis (2D-DIGE) coupled with matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF ms) of CD patients with varying degrees of histological abnormalities defined by Marsh criteria and controls. Our results clearly evidenced the presence of two groups of patients: Group A, including controls and Marsh 0-I CD patients; and Group B, consisting of CD subjects with grade II-III Oberhuber-Marsh classification. Differentially expressed proteins were involved mainly in lipid, protein and sugar metabolism. Interestingly, in Group B, several downregulated proteins (FABP1, FABP2, APOC3, HMGCS2, ACADM and PEPCK) were implicated directly in the peroxisome proliferator-activated receptor (PPAR) signaling pathway. Moreover, Group B patients presented a deregulation of some proteins involved in apoptosis/survival pathways: phosphatidylethanolamine-binding protein 1 (PEBP1), Ras-related nuclear protein (Ran) and peroxiredoxin 4 (PRDX4). PEBP1 downregulation and RAN and PRDX4 upregulation were associated with more severe tissue damage. Likewise, IgMs were found strongly upregulated in Group B. In conclusion, our results indicate that a downregulation of proteins involved in PPAR signaling and the modulation of several cancer-related proteins are associated with the highest CD histological score according to Oberhuber-Marsh classification.

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“…There was no detectable secretion of pIgA unlinked to secretory component as shown by sucrose density gradient ultracentrifugation. Secretory component in perfusate samples of coeliac disease sedimented mainly with pIgA, but 14-4% (range 7-27) was excreted as free secretory component compared to 6-9% (range[4][5][6][7][8][9][10][11][12][13][14][15][16][17] in controls (p=0 05). The albumin and IgG secretion rates were similar in patients and control subjects.…”

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confidence: 99%

Jejunal immunoglobulin and antigliadin antibody secretion in adult coeliac disease.

Colombel

Mascart‐Lemone

Németh

et al. 1990

Gut

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We compared the local intestinal immunoglobulin (Ig) secretion in six adult patients with coeliac disease and nine control subjects by perfusion of a small bowel segment under an occluding balloon and analysis ofthe perfusion fluid for the content of Ig and secretory component. The results were compared to the number of Ig-containing plasma cells in the test segment. There was, respectively, a two-

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Immunoglobulin secretion by isolated intestinal lymphocytes: spontaneous production and T-cell regulation in normal small intestine and in patients with coeliac disease.

Cited by 21 publications

References 20 publications

Proteomic Analyses Lead to a Better Understanding of Celiac Disease: Focus on Epitope Recognition and Autoantibodies

Proteomic Analyses Lead to a Better Understanding of Celiac Disease: Focus on Epitope Recognition and Autoantibodies

PPAR Signaling Pathway and Cancer-Related Proteins Are Involved in Celiac Disease-Associated Tissue Damage

Jejunal immunoglobulin and antigliadin antibody secretion in adult coeliac disease.

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