Immunoglobulin (IgG and IgA) and complement (C3) concentrations in uterine secretion following an intrauterine challenge of Streptococcus Zooepidemicus in mares susceptible to versus resistant to chronic uterine infection1

Abstract

The validity of measuring concentrations of immunoglobulins in undiluted uterine secretions was established. The concentrations of IgG, IgA, and cleavage factor C3 of the complement system in uterine secretions were compared in mares with different resistance to chronic uterine infection (CUI). The uteri of mares susceptible (n = 6) and resistant to CUI (n = 5) were inoculated with 5 x 10(6) Streptococcus zooepidemicus when the mares were in estrus. Uterine secretions were sampled, and sampling was immediately followed by a uterine lavage at 5 and 24 h after bacterial inoculation. During a subsequent estrus, bacterial inoculation of the uterus was repeated, and samples were taken from the mares 12 and 36 h later. The fraction of uterine secretion in each uterine washing was determined by dividing the protein concentration of the undiluted uterine secretion by the protein concentration of the corresponding uterine washing. There was a significant correlation between measured concentrations of immunoglobulins in uterine secretions and calculated concentrations in the uterine washings (p < 0.05). Concentrations of IgG and C3 in uterine secretions declined similarly in both susceptible and resistant mares during the first 24 h after bacterial inoculation (p < 0.04). In contrast to the susceptible group, which showed a continuous decline at 36 h, resistant mares demonstrated an increased concentration of both IgG and C3 at this time. Concentrations of IgA did not differ between susceptible and resistant mares. It was concluded that the described method of sampling uterine secretions was useful for analyzing IgG and IgA.(ABSTRACT TRUNCATED AT 250 WORDS)

Effects of different postbreeding treatments on fertility of thoroughbred mares

“…However, several researchers have proposed that impaired uterine physical clearance, i. e., defective uterine contractility and lymphatic drainage, and local cellular and humoral immunity prevent some mares from eliminating contaminants. These problems characterize mares as susceptible to endometritis (Cheung et al, 1985;Evans et al, 1986;Allen & Pycock, 512 1988;LeBlanc et al, 1989;Liu, 1991 and1992;Troedsson et al, 1993aTroedsson et al, , 1993bTroedsson et al, and 1995LeBlanc et al, 1994 and1995). Uterine lavage with plasma, oxytocin injections and combination of both have been recommended as treatments for postbreeding endometritis (Asbury, 1986;Pascoe, 1995).…”

Interaction between spermatozoa and the mare’s reproductive is mediated by biochemical, physical, and inflammatory mechanisms

“…In vitro studies suggest that spermatozoa induce PMN-chemotaxis via complement activation (Figure 3; Troedsson et al 1995a, Rozeboom et al 2001). Complement activity as well as isolation of complement cleavage products has been demonstrated in the equine reproductive tract (Asbury et al 1982, Watson et al 1987, Troedsson et al 1993, and this is likely involved in the mechanism by which breeding-induced endometritis is induced. Activated PMNs bind to spermatozoa and although the mechanism of this binding is not yet fully understood, recent data suggest that it is mediated by extracellular neutrophil traps (NET), but also involves more traditional ligand receptor binding Foster 2005, Brinkman et al 2004).…”

Kısraklarda Tek ve Çift Taze Sperm ile Tohumlama Sonrası Uterin İmmun Yanıt

“…Shortly after breeding or introduction of pathogens to the uterine lumen, polymorphonucleated neutrophils (PMN) migrate into the uterine lumen and intrauterine fluid containing inflammatory mediators accumulates [3] . Activation of the innate immune system acts in combination with mechanical clearance to assist the uterine lumen to clear the local inflammatory response following breeding.…”