Immunoglobulin G purification from bovine serum with pseudo-specific supermacroporous cryogels

Attieh, Mira Daoud; Chaib, Haidar; Armutçu, Canan; Uzun, Lokman; Elkak, Assem; Deni̇zli̇, Adil

doi:10.1016/j.seppur.2013.08.026

Cited by 41 publications

(26 citation statements)

References 29 publications

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“…Third, particles embedding into a cryogel network caused an increase in the specific surface area, which is one of the most efficient parameters on the adsorption capacity. 4,41,42 Finally, the combination of these features formed a synergy to get higher adsorption capacities with fast and efficient adsorption kinetics.…”

Section: Methodsmentioning

confidence: 99%

Particle-Assisted Ion-Imprinted Cryogels for Selective Cd^IIIon Removal

Tabaklı

Topçu

Döker

et al. 2015

Ind. Eng. Chem. Res.

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Herein, we have reported particle-assisted ion-imprinted cryogels, which were synthesized by embedding finely crushed functional particles into cryogel structure under semifrozen conditions. These cryogels showed high adsorption efficiency and affinity against Cd II ions. Cd II adsorption performances were evaluated by varying some effective factors. In order to analyze the data, we applied Langmuir and Freundlich adsorption isotherms while using three different kinetic models, pseudo-first-order, pseudo-second-order, and Weber−Morris as well. Also, the dimensionless equilibrium parameter (R L ), initial adsorption rate, and half-adsorption time were calculated. The results revealed that ion-imprinted-polymer (IIP) cryogels have homogeneously distributed cavities, which were formed by a particle-assisted imprinting process, and the theoretical maximum adsorption capacity (Q max , 35.97 μg/g) was very close to the experimental value (Q eq , 32.15 μg/g). In addition, R L values for both IIP and nonimprinted-polymer cryogels showed favorability of the adsorption process, while kinetic models indicated that there were no diffusion limitations during the adsorption process, which means that the rate-limiting step was chemosorption of heavy-metal ions on binding sites (imprinted cavities or functional groups) with a high initial adsorption rate and a low half-adsorption time. Desorption, reusability, and selectivity studies were also conducted to state the performance of the cryogels. In conclusion, this promising approach provides a novel methodology for selective Cd II removal from water sources.

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Section: Methodsmentioning

confidence: 99%

Particle-Assisted Ion-Imprinted Cryogels for Selective Cd^IIIon Removal

Tabaklı

Topçu

Döker

et al. 2015

Ind. Eng. Chem. Res.

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“…The reagents acrylamide (AAm) and 2‐hydroxyethyl methacrylate (HEMA) were used as the precursor of the affinity cryogel because of their neutrality and inert nature, which minimized nonspecific interactions with proteins. By controlling the freezing temperature and the precursor concentration, the pore size of cryogels was in the range of 50‐200 µm [45,46], which was not large enough for the purification from a viscous solution. Formation of cryogels with larger pore sizes by decreasing the concentration of the monomer requires a sacrifice in the cryogel mechanical strength, which cannot be applied in practice.…”

Section: Introductionmentioning

confidence: 99%

Preparation of supermacroporous cryogels with improved mechanical strength for efficient purification of lysozyme from chicken egg white

Sun

Feng

Zhong

et al. 2020

J of Separation Science

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A novel, facile, and robust strategy was proposed to increase the pore size and mechanical strength of cryogels. By mixing the monomers of acrylamide and 2‐hydroxyethyl methacrylate as the precursor, a monolithic copolymer cryogel with large interconnected pores and thick pore walls was prepared. Hydrogen bonding between the two monomers contributed to the entanglement and aggregation of the copolymers, thickening the pore walls and resulting in larger pore sizes. Analysis via mercury porosimetry demonstrated that the interconnected pore diameter of the copolymer cryogel ranged from 10‐350 µm, which was far larger than that of the cryogels from one monomer (10‐50 µm). Additionally, the thicker pore walls of the copolymer cryogel improved its mechanical strength. Affinity cryogels were prepared through covalent immobilization using Tris(hydroxymethyl)aminomethane as a coupling agent, and the affinity binding of lysozymes on Tris‐cryogel was evaluated by the Langmuir isothermal adsorption with the maximum adsorption capacity of 360 mg/g. Compared with that of the Tris‐cryogels produced from one monomer, the copolymer Tris‐cryogel exhibited higher adsorption capacity and lysozyme purity, when the chicken egg white solution flowed solely driven by gravity. This work provides a new avenue for designing and developing supermacroporous cryogels for bioseparation.

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“…The adsorption kinetics experiments were carried out in duplicate at room temperature as described in Daoud‐Attieh et al (). The C10/10 column (GE Healthcare, USA) packed with 1.0 mL of P‐Tyr‐agarose was equilibrated with NaP 10 mmol L −1 , pH 6.0 (adsorption buffer) at 1.0 mL min −1 (superficial velocity of 76.4 cm h −1 ).…”

Section: Methodsmentioning

confidence: 99%

“…The pseudo‐first‐order and pseudo‐second‐order kinetic models were applied to the experimental data on the adsorption kinetics of human IgG using the following equations (Daoud‐Attieh et al, ; Oliveira et al, ):

q_{t} = q_{e} ((), 1 + e^{- k_{1} t})

q_{t} = \frac{k_{2} q_{e}^{2} t}{(1 + t k_{2} q_{normale})}

in which q e and q t are the amount of IgG adsorbed (mg) onto P‐Tyr‐agarose (g) at equilibrium and time t (min), respectively; k 1 (min −1 ) is the rate constant for the pseudo‐first‐order adsorption kinetics and k 2 (g mg −1 min −1 ) is the rate constant for the pseudo‐second‐order kinetics.…”

Section: Methodsmentioning

confidence: 99%

Phospho‐l‐tyrosine‐agarose chromatography: Adsorption of human IgG and its proteolytic fragments

Pavan

Bresolin

Muzio

et al. 2018

Biomedical Chromatography

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The behavior of human immunoglobulin G (IgG) and antigen-binding fragment (Fab fragment) adsorption onto phospho-L-tyrosine immobilized on agarose (P-Tyr-agarose) was evaluated by pseudoaffinity chromatography. The effects of buffer systems MES, MOPS, Bis-Tris, Tris-HCl and sodium phosphate (NaP) and pH on IgG adsorption were studied and high purity values were obtained (96%, based on ELISA analysis of albumin, transferrin and immunoglobulins A, G and M) when IgG was purified from human plasma diluted in 10 mmol L −1 NaP buffer at pH 6.0. The capture of IgG by the P-Tyragarose was also promising, since 91% of the IgG was adsorbed when plasma was diluted in 25 mmol L −1 MES buffer at pH 5.5, recommending its use for IgG depletion from human plasma under this condition. The experimental data on IgG adsorption kinetics were in agreement with the pseudo-second-order model. The adsorption isotherm data were well described by the Langmuir-Freundlich model with the value of parameter n being <1 (0.72), indicating negative cooperativity. Selectivity was achieved on P-Tyr-agarose from digested human IgG in HEPES 25 mmol L −1 buffer at pH 7.0where Fab fragments were obtained in eluted fractions without Fc fragments (but with uncleaved IgG) with 86.2% recovery.

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Immunoglobulin G purification from bovine serum with pseudo-specific supermacroporous cryogels

Cited by 41 publications

References 29 publications

Particle-Assisted Ion-Imprinted Cryogels for Selective Cd^IIIon Removal

Particle-Assisted Ion-Imprinted Cryogels for Selective Cd^IIIon Removal

Preparation of supermacroporous cryogels with improved mechanical strength for efficient purification of lysozyme from chicken egg white

Phospho‐l‐tyrosine‐agarose chromatography: Adsorption of human IgG and its proteolytic fragments

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Immunoglobulin G purification from bovine serum with pseudo-specific supermacroporous cryogels

Cited by 41 publications

References 29 publications

Particle-Assisted Ion-Imprinted Cryogels for Selective CdIIIon Removal

Particle-Assisted Ion-Imprinted Cryogels for Selective CdIIIon Removal

Preparation of supermacroporous cryogels with improved mechanical strength for efficient purification of lysozyme from chicken egg white

Phospho‐l‐tyrosine‐agarose chromatography: Adsorption of human IgG and its proteolytic fragments

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Particle-Assisted Ion-Imprinted Cryogels for Selective Cd^IIIon Removal

Particle-Assisted Ion-Imprinted Cryogels for Selective Cd^IIIon Removal