Immunoglobulin G‐dependent changes in basophil allergen threshold sensitivity during birch pollen immunotherapy

Lalek, Nika; Košnik, Mitja; Šilar, Mira; Korošec, Peter

doi:10.1111/j.1365-2222.2010.03524.x

Cited by 56 publications

(81 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similarly, Lalek et al [33] demonstrated that perennial SCIT with a birch pollen allergoid led to reduced CD63 expression, which correlated with patient symptoms assessed by a visual analog scale. They also firstly revealed the reduced inhibitory effect of the patient’s serum after elimination of the IgG antibodies.…”

Section: Discussionmentioning

confidence: 99%

“…This study stresses the prominent role of IgG antibodies in decreased basophil activation to allergen challenge in the basophil activation test. The blocking IgG antibodies most likely bind to allergen and thus reduce free allergen concentrations in the vicinity of mast cells and basophils [33]. Cady et al [34] demonstrated that IgG antibodies produced during subcutaneous immunotherapy with a cat extract also inhibited basophil responses to allergen via binding to both FcγRIIA and inhibitor FcγRIIB immunoglobulin Fc receptors expressed by basophils.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Desensitization Effect of Preseasonal Seven-Injection Allergoid Immunotherapy with Olive Pollen on Basophil Activation: The Efficacy of Olive Pollen-Specific Preseasonal Allergoid Immunotherapy on Basophils

Gökmen¹,

Ersoy²,

Gülbahar³

et al. 2012

Int Arch Allergy Immunol

View full text Add to dashboard Cite

Background: It has previously been demonstrated that subcutaneous immunotherapy with allergoids positively affects clinical and immunological parameters even after 7 preseasonal injections. However, its effect on basophil activation remains unclear. We investigated the effect of preseasonal allergoid immunotherapy on basophils and concomitantly assessed its clinical and immunological efficacy in olive pollen-monosensitized patients. Methods: This study enrolled 437 consecutive patients with respiratory allergy and positive skin prick tests (SPTs); 212 (48.5%) patients were sensitized to olive pollen, and 33 (7.5%) patients were sensitized to olive pollen only. Of these patients, 23 received preseasonal immunotherapy with an olive pollen allergoid. The olive pollen-specific basophil activation, the titrated nasal provocation test, the nasal symptom score, and olive pollen-specific IgE, IgG1 and IgG4 levels were evaluated before immunotherapy and 8 months after the end of immunotherapy in the follow-up visit. Results: In comparison to baseline evaluation, 7 preseasonal injections of an allergoid resulted in a significant decrease in the percentage of basophils expressing CD63 (29 vs. 7%, respectively, p < 0.0001) and a significant increase in the titrated nasal provocative dose (1/10 vs. 1/1, respectively, p < 0.01). SPT induration diameters caused by an olive pollen extract decreased (12 mm at baseline vs. 5.5 mm at follow-up, p < 0.005), as did nasal symptom score (7 at baseline vs. 3 at follow-up, p < 0.01). Olive pollen-specific IgE (17.5 vs. 50 kU/l, p < 0.012), IgG1 (0.16 vs. 2.9 µg/ml, p < 0.0001) and IgG4 (0.07 vs. 1.92 µg/ml, p < 0.0001) levels significantly increased. Conclusions: Immunotherapy with 7 preseasonal injections of an olive pollen allergoid decreases olive pollen-specific basophil activation over 8 months, an effect observed in vitro and in vivo.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Desensitization Effect of Preseasonal Seven-Injection Allergoid Immunotherapy with Olive Pollen on Basophil Activation: The Efficacy of Olive Pollen-Specific Preseasonal Allergoid Immunotherapy on Basophils

Gökmen¹,

Ersoy²,

Gülbahar³

et al. 2012

Int Arch Allergy Immunol

View full text Add to dashboard Cite

show abstract

“…The suppression of basophil activation after discontinuation of AIT has been demonstrated 12-24 months following discontinuation of treatment [Nopp et al 2009;Lalek et al 2010;Gokmen et al 2012;Shamji et al 2015;Zidarn et al 2015].…”

Section: Cellular Changesmentioning

confidence: 99%

Applications and mechanisms of immunotherapy in allergic rhinitis and asthma

Kappen

Durham

Veen

et al. 2016

Ther Adv Respir Dis

View full text Add to dashboard Cite

Clinical and immunologic tolerance are hallmarks of successful allergen immunotherapy (AIT). Clinical benefits such as reduced symptoms, pharmacotherapy intake and improvement of quality of life persist following cessation of treatment. Successful AIT is associated with suppression of allergic inflammatory cells such as mast cells, eosinophils and basophils in target organs. Furthermore, AIT down-regulates type 2 innate lymphoid cells and allergen-specific type 2 T-helper (Th2) cells. The immunologic tolerant state following AIT is associated with the induction of distinct phenotypes of regulatory T-cells (T-regs) including interleukin (IL)-10-, IL-35-and transforming growth factor (TGF)-β-producing T-regs and FoxP3 + T-regs. B-cell responses, including the induction of IL-10 + regulatory B-cells (B-regs) and the production of IgG4-associated blocking antibodies are also induced following successful AIT. These events are associated with the suppression of antigen-specific Th2 responses and delayed immune deviation in favour of Th1 type responses. Insight into the mechanisms of AIT has allowed identification of novel biomarkers with potential to predict the clinical response to AIT and also novel therapeutic strategies for more effective and safer AIT.

show abstract

“…In the third study [4], blood samples were taken at a median delay of 833 days after the onset of sCAD symptoms. Lindgren et al [18] reported an increase in the median tHcy level from 11.4 to 14.5 µmol/l from the acute (mean 2 days after stroke onset) to the chronic phase (median 583 days after stroke onset). Thus, the later blood sampling might explain the higher tHcy values and prevalence of mild hyperhomocysteinemia reported in previous studies [3,4,5].…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, elevated tHcy could be the consequence of cerebral ischemia or also an infection which has been shown to be associated with sCAD [21, 22] rather than a cause of the disease. Also, food deprivation increases the tHcy levels [18, 23, 24]. In acute stroke, patients are kept fasting, and tHcy may therefore increase independently of the ischemic process.…”

Section: Discussionmentioning

confidence: 99%

Increased Prevalence of Hyperhomocysteinemia in Cervical Artery Dissection Causing Stroke

et al. 2009

View full text Add to dashboard Cite

Background: Spontaneous cervical artery dissection (sCAD) is a nonatherosclerotic vascular disease of unknown etiology. Mild elevation of total plasma homocysteine (tHcy) levels may be a risk factor for sCAD, but the precise mechanism remains unknown. On the other hand, mild hyperhomocysteinemia is also associated with ischemic stroke related to atherothrombotic or small artery disease. We undertook a case-control study to compare the prevalence of mild hyperhomocysteinemia and tHcy levels between patients with a first ischemic stroke due to sCAD and healthy volunteers, as well as patients with a first ischemic stroke due to atherothrombotic or small artery disease. Methods: Fasting tHcy levels were determined in 346 consecutive patients with a first ischemic stroke due to sCAD (n = 86) and atherothrombotic or small artery disease (n = 260) within 24 h after the onset of symptoms, and in 100 healthy volunteers. Results: Mild hyperhomocysteinemia was more prevalent in patients with sCAD causing ischemic stroke (n = 33, 38%) than in healthy volunteers (n = 23, 23%; p = 0.034), and less prevalent than in patients with ischemic stroke due to atherothrombotic or small artery disease (n = 149, 57%; p = 0.001). Mean fasting tHcy levels of patients with ischemic stroke caused by sCAD showed a trend to be higher (11.4 ± 3.8 μmol/l) than those of healthy volunteers (10.2 ± 3.0 μmol/l, p = 0.61), but were lower than those of patients with stroke due to atherothrombotic or small artery disease (13.6 ± 6.6 μmol/l, p = 0.002). Conclusion: Our results suggest that mild hyperhomocysteinemia may be a risk factor for sCAD causing ischemic stroke, but further studies are needed to identify a possible mechanism. This study confirms the association of hyperhomocysteinemia with ischemic stroke due to atherothrombotic or small artery disease.

show abstract

Immunoglobulin G‐dependent changes in basophil allergen threshold sensitivity during birch pollen immunotherapy

Abstract: In this study, we showed that birch immunotherapy-induced IgG antibodies are associated with a reduction in basophil allergen threshold sensitivity. Further studies are needed to show whether the changes in basophil sensitivity are of clinical relevance in pollen immunotherapy.

Cited by 56 publications

References 32 publications

Desensitization Effect of Preseasonal Seven-Injection Allergoid Immunotherapy with Olive Pollen on Basophil Activation: The Efficacy of Olive Pollen-Specific Preseasonal Allergoid Immunotherapy on Basophils

Desensitization Effect of Preseasonal Seven-Injection Allergoid Immunotherapy with Olive Pollen on Basophil Activation: The Efficacy of Olive Pollen-Specific Preseasonal Allergoid Immunotherapy on Basophils

Applications and mechanisms of immunotherapy in allergic rhinitis and asthma

Increased Prevalence of Hyperhomocysteinemia in Cervical Artery Dissection Causing Stroke

Contact Info

Product

Resources

About