Immunoglobulin G antibody capture enzyme-linked immunosorbent assay: a versatile assay for detection of anti-human immunodeficiency virus type 1 and 2 antibodies in body fluids

Thongcharoen, Prasert; Wasi, Chantapong; Louisirirotchanakul, Suda; Parry, John; Connell, J.; Mortimer, P P

doi:10.1128/jcm.30.12.3288-3289.1992

Cited by 30 publications

(18 citation statements)

References 4 publications

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“…This study represents the first OraSure OF specimen set to be evaluated by GACELISA, a capture-antibody assay designed for OF, urine, and dried blood spot specimens. In other studies, GACELISA has consistently provided sensitivities and specificities comparable to those found with matched serum tests using whole saliva as well as Omni-Sal (Saliva Diagnostic Systems, Vancouver, Wash.) and other OF specimens (8,11,12,15,16,25,27,30,32,37). GACELISA has also been shown to discriminate between HIV antibody-positive and HIV antibody-negative spec-imens despite the low IgG concentrations in OF specimens (20).…”

Section: Discussionmentioning

confidence: 64%

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Detection of Antibodies to Human Immunodeficiency Virus Type 1 in Oral Fluids: A Large-Scale Evaluation of Immunoassay Performance

Granade

Phillips

Parekh

et al. 1998

Clin Diagn Lab Immunol

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Paired serum and oral-fluid (OF) specimens (n = 4,448) were collected from blood donors and patients attending local sexually transmitted disease clinics in Trinidad and Tobago and the Bahamas and were tested for the presence of human immunodeficiency virus type 1 (HIV-1) antibodies. Sera were tested by Abbott AB HIV-1/HIV-2 (rDNA) enzyme immunoassay (EIA), and positive specimens were confirmed by Cambridge HIV-1 and HIV-2 Western blotting (WB). OF specimens were collected with the OraSure collection device and were tested by Murex GACELISA and by two EIAs from Organon Teknika (the Oral Fluid Vironostika HIV-1 Microelisa System [OTC-L] and the Vironostika HIV-1 Microelisa System [OTC-M]). EIA-reactive OF specimens were confirmed by miniaturized WB (OFWB). GACELISA detected all 474 HIV-1 seropositive specimens (sensitivity, 100%). OTC-L detected 470 positive specimens (sensitivity, 99.2%), while OTC-M detected 468 positive specimens (sensitivity, 98.8%). Specificities ranged from 99.2 to 100% for the three assays. Concordance of OFWB with serum WB was 99.4%, and banding patterns determined by the two methods were similar. The immunoglobulin G (IgG) concentration of OF specimens ranged from 0.21 to 100 μg/ml, with a mean of 17.1 μg/ml. Significant differences in OF IgG concentrations were observed between HIV antibody-positive and HIV antibody-negative persons (31.94 versus 15.28 μg/ml, respectively [P < 0.0001]). These data further confirm the suitability of OF specimens for detection of HIV-1 antibodies. Currently available HIV-1 antibody assays provide sensitivities and specificities with OF specimens comparable to those achieved with serum specimens.

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Section: Discussionmentioning

confidence: 64%

“…The low concentration of IgG in the oral cavity (approximately 0.10% of the concentration of IgG in serum) significantly affected early studies of EIA performance with OF specimens (1,3,12,37). Mortimer and Parry suggested a minimal FIG.…”

Section: Discussionmentioning

confidence: 99%

Detection of Antibodies to Human Immunodeficiency Virus Type 1 in Oral Fluids: A Large-Scale Evaluation of Immunoassay Performance

Granade

Phillips

Parekh

et al. 1998

Clin Diagn Lab Immunol

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“…The sensitivity and specificity of GACELISA were 88. I-99.4% and 97.9-loo%, respectively (23)(24)(25). Those of GACPAT were 92.5-100% and 97.9-99.6%, respectively (23, 24).…”

Section: -1mentioning

confidence: 99%

“…Anti-HIV-1 antibodies in urine were detected previously by the conventional ELISA (20)(21)(22), IgG antibody-capture enzyme-linked immunosorbent assay (GACELISA) (23)(24)(25) and IgG antibody-capture particle adherence test (GACPAT) (23,24). The sensitivity and specificity of the conventional loo A AA AAA…”

Section: Comparison With Previous Reportsmentioning

confidence: 99%

Detection of antibody IgG to HIV‐1 in urine by ultrasensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) using recombinant p24 as antigen for diagnosis of HIV‐1 infection

Hashida

Hashinaka

Hirota

et al. 1994

Clinical Laboratory Analysis

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Anti-HIV-1 IgG in urine was detected by an ultrasensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) using recombinant p24 gag protein (p24) of HIV-1 as antigen and beta-D-galactosidase from Escherichia coli as label. Anti-HIV-1 IgG in urine was reacted simultaneously with 2,4-dinitrophenyl-bovine serum albumin-recombinant p24 conjugate and recombinant p24-beta-D-galactosidase conjugate. The complex formed, consisting of the three components, was trapped onto polystyrene balls coated with affinity-purified (anti-2,4-dinitrophenyl group) IgG, eluted with epsilon N-2,4-dinitrophenyl-L-lysine, and transferred to polystyrene balls coated with affinity-purified (anti-human IgG gamma-chain) IgG. Bound beta-D-galactosidase activity was assayed by fluorometry. This assay was at least 3,000-fold more sensitive than conventional methods. The lowest signal among 49 asymptomatic carriers was 3.1-fold higher than the highest nonspecific signal among 100 seronegative subjects. The sensitivity and specificity were both 100%. The positivity could be confirmed by preincubation of urine samples with excess of the antigen. Thus, this assay would be a powerful tool for detecting IgG antibody to HIV-1 in urine.

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“…Anti-HIV-1 antibodies in urine were detected previously by the conventional ELISA (17)(18)(19), IgG antibody-capture enzyme-linked immunosorbent assay (GACELISA) (20)(21)(22) and IgG antibody-capture particle adherence test (GACPAT) (20,2 I ) . The sensitivity and specificity of the conventional ELISA performed using various commercial kits were 93 -100% and 92-loo%, respectively (17-19j.…”

Section: Comparison With Previous Reportsmentioning

confidence: 99%

Detection of antibody IgG to HIV‐1 in urine by sensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) using recombinant proteins as antigens for diagnosis of HIV‐1 infection

Hashida

Hirota

Hashinaka

et al. 1993

Clinical Laboratory Analysis

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For diagnosis of HIV-1 infection, attempts were made to detect anti-HIV-1 IgG in urine by sensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) using recombinant reverse transcriptase (RT) and p17 as antigens. Anti-HIV-1 IgG in urine was reacted simultaneously with 2,4-dinitrophenyl-bovine serum albumin-recombinant protein conjugate and recombinant protein-enzyme conjugate. The enzymes used as labels were horseradish peroxidase for RT and Escherichia coli beta-D-galactosidase for p17. The complex formed, consisting of the three components, was trapped onto polystyrene balls coated with affinity-purified (anti-2,4-dinitrophenyl group) IgG, eluted with epsilon N-2,4-dinitrophenyl-L-lysine and transferred to polystyrene balls coated with affinity-purified (anti-human IgG gamma-chain) IgG. Finally, bound enzyme activity was assayed by fluorometry. Urine samples were collected from 100 seronegative subjects and 70 seropositive subjects. The sensitivity and specificity were both 100% with unconcentrated urine samples. The positivity was confirmed by preincubation of urine samples with excess of the antigens. The positivity and negativity with one of the two antigens could be confirmed with the other antigen. The positivity with low signals could be confirmed by concentration of urine samples. Detection of anti-HIV-1 IgG in urine by the immune complex transfer enzyme immunoassay using different antigens would make diagnosis of HIV-1 infection possible.

show abstract

Immunoglobulin G antibody capture enzyme-linked immunosorbent assay: a versatile assay for detection of anti-human immunodeficiency virus type 1 and 2 antibodies in body fluids

Cited by 30 publications

References 4 publications

Detection of Antibodies to Human Immunodeficiency Virus Type 1 in Oral Fluids: A Large-Scale Evaluation of Immunoassay Performance

Detection of Antibodies to Human Immunodeficiency Virus Type 1 in Oral Fluids: A Large-Scale Evaluation of Immunoassay Performance

Detection of antibody IgG to HIV‐1 in urine by ultrasensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) using recombinant p24 as antigen for diagnosis of HIV‐1 infection

Detection of antibody IgG to HIV‐1 in urine by sensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) using recombinant proteins as antigens for diagnosis of HIV‐1 infection

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