Immunoglobulin Free Light Chains in Immune Responses

Veeken, Joris der; Redegeld, Frank A.

doi:10.2174/157339508784325055

Cited by 1 publication

(1 citation statement)

References 176 publications

(218 reference statements)

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“…Finally, it might be of interest to test for the reactivity of CSF-restricted oligoclonal fLC bands with microbial antigens. In our experience, CSF-restricted oligoclonal fLC are common in neuroborreliosis and there remains a possibility that oligoclonal fLC (dimers) may have antigen-binding capability [ 34 , 35 ], although this has been disputed by others [ 36 ]. In our case, this opportunity was missed.…”

Section: Discussionmentioning

confidence: 99%

Detection of oligoclonal IgG kappa and IgG lambda bands in cerebrospinal fluid and serum with Hevylite™ antibodies. comparison with the free light chain oligoclonal pattern

Zeman

Hradílek

Švagera

et al. 2012

Fluids Barriers CNS

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Background Oligoclonal IgG bands in cerebrospinal fluid that are absent in serum indicate intrathecal IgG synthesis and are a sensitive marker of CNS inflammatory diseases, in particular multiple sclerosis. It may be of interest to determine whether these bands are predominantly IgGκ or IgGλ. Methods We have used Hevylite™ antibodies and developed a technique for detection of oligoclonal IgGκ and IgGλ bands by means of isoelectric focusing followed by immunoblotting. The same technique was used for oligoclonal free κ and free λ detection. Among several techniques tested, affinity immunoblotting appears to be the most sensitive; it can detect less than 1 ng of IgGκ or IgGλ paraprotein. We compared oligoclonal IgG profiles with those of oligoclonal IgGκ and IgGλ. There was good agreement concerning the presence or absence of intrathecal synthesis. We observed the ratios between oligoclonal IgGκ and IgGλ bands, and they did not always match the ratios between free κ and free λ bands. We were also able to detect antigen-specific CSF-restricted oligoclonal IgGκ and IgGλ bands in neuroborreliosis. It remains to be determined subsequently by a clinically-oriented prospective study, whether predominant IgGκ/IgGλ or free κ/free λ can be observed more frequently in particular diseases with oligoclonal IgG synthesis. Discussion Very sensitive detection of oligoclonal IgGκ and IgGλ bands in cerebrospinal fluid with Hevylite antibodies is feasible; detection of antigen-specific IgGκ or IgGλ is possible as well. In particular situations, e.g. when difficulties arise in distinguishing between oligoclonal and monoclonal pattern, the test may be of considerable clinical value.

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Section: Discussionmentioning

confidence: 99%