Immunoglobulin E production in mice by means of contact sensitization with a simple chemical, hapten

Nagai, Naoki; Matsuo, Akihiko; Hiyama, Hidetaka; Inagaki, Nobuya; Kawada, Kenji

doi:10.1016/s0091-6749(97)70003-4

Cited by 79 publications

(74 citation statements)

References 16 publications

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“…Allergic dermatitis was induced by repeated topical application with DNFB as described previously (Nagai et al, 1997b). Briefly, 25 l of 0.15% DNFB in acetone/ olive oil (3:1) was applied to each side of right ears of C3H/HeN mice 6 times at 7-day intervals.…”

Section: Dnfb-induced Dermatitismentioning

confidence: 99%

“…Interestingly, in mice, repeated application of contact sensitizing agents, such as 2,4-dinitrofluorobenzene (DNFB) and trinitrochlorobenzene, develops an immediate-type reaction, in contrast to a single application that causes a typical delayed-type reaction (Kitagaki et al, 1995(Kitagaki et al, , 1997. Repeated elicitation with such agents not only increases the serum level of IgE but also induces Th2-type cytokine expression (Nagai et al, 1997b). In addition, mast cell accumulation occurs in the dermis because of the repeated challenge (Kitagaki et al, 1995), as is often observed in AD patients.…”

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confidence: 99%

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Chymase Participates in Chronic Dermatitis by Inducing Eosinophil Infiltration

Tomimori

Muto

Fukami

et al. 2002

Laboratory Investigation

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SUMMARY:An epicutaneous application of 2,4-dinitrofluorobenzene (DNFB) to a mouse ear caused a transient skin swelling, and the repetition of the challenge enlarged the contact dermatitis. The repeated challenge with DNFB also induced eosinophil infiltration on the application site. Administration of a chymase inhibitor significantly inhibited the ear swelling as well as eosinophil accumulation. An intradermal injection of human chymase to the mouse ear also elicited transient skin swelling and eosinophil infiltration, both of which were augmented in proportion to the number of injections. Human serum albumin and heat-inactivated chymase failed to induce such skin reactions, suggesting the participation of proteolytic activity of the enzyme. In addition, chymase stimulated eosinophil migration in vitro in a concentration-dependent manner. Taken together, these observations suggest that mast cell chymase may contribute to development of the DNFB-induced dermatitis, probably by promoting eosinophil infiltration. It is therefore possible that chymase plays a role in pathogenesis of chronic dermatitis such as atopic dermatitis. (Lab Invest 2002, 82:789 -794).A topic dermatitis (AD) is a chronic eczematous skin disorder characterized by dry and itchy skin. The patients with AD often have a family history of other allergic diseases including asthma and hay fever. Although AD is known to be a manifestation of immediate hypersensitivity mediated by IgE, delayed-type hypersensitivity is also involved in the skin reaction of the patients (Tanaka et al, 1994;Varela et al, 1999). Interestingly, in mice, repeated application of contact sensitizing agents, such as 2,4-dinitrofluorobenzene (DNFB) and trinitrochlorobenzene, develops an immediate-type reaction, in contrast to a single application that causes a typical delayed-type reaction (Kitagaki et al, 1995(Kitagaki et al, , 1997. Repeated elicitation with such agents not only increases the serum level of IgE but also induces Th2-type cytokine expression (Nagai et al, 1997b). In addition, mast cell accumulation occurs in the dermis because of the repeated challenge (Kitagaki et al, 1995), as is often observed in AD patients. Therefore, this animal model is thought to be valuable for studying human AD.Chymase is a chymotrypsin-like serine protease and is primarily stored in secretory granules of mast cells (Schwartz and Austen, 1980). Chymase cleaves a variety of physiological substances, including metalloproteases (Fang et al, 1997), procollagen (Kofford et al, 1997), precursor of interleukin 1␤ (IL-1␤) (Mizutani et al, 1991b), and membrane-associated stem cell factor (Longley et al, 1997), while its precise role is not clear. Human mast cells are classified into two types, MC TC and MC T , based on their composition of serine protease (Irani et al, 1986). MC TC contains both chymase and tryptase, while MC T expresses tryptase but not chymase. Because MC TC predominates in the skin (Irani et al, 1989), chymase has been implicated in the pathogenesis of skin disorders complic...

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Section: Dnfb-induced Dermatitismentioning

confidence: 99%

mentioning

confidence: 99%

Chymase Participates in Chronic Dermatitis by Inducing Eosinophil Infiltration

Tomimori

Muto

Fukami

et al. 2002

Laboratory Investigation

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“…It is also used as a sensitizing agent and hapten in laboratory immunology, and as a reagent for identifying the terminal amino acids in a protein chain 1,2) . The repeated application of DNFB to the ears of mice results in a typical allergic dermatitis and the simultaneous production of IgE antibody to DNFB 3,4) . Repeated application of DNFB to the mouse ear causes the ear to swell accompanied by a rise in serum specific IgE levels 4) .…”

Section: Introductionmentioning

confidence: 99%

“…The repeated application of DNFB to the ears of mice results in a typical allergic dermatitis and the simultaneous production of IgE antibody to DNFB 3,4) . Repeated application of DNFB to the mouse ear causes the ear to swell accompanied by a rise in serum specific IgE levels 4) . Thickening of the epidermis, formation of scabs, and infiltration of abundant inflammatory cells are also induced 5) .…”

Section: Introductionmentioning

confidence: 99%

“…Because magnetic infrared laser (MIL) therapy has also been used in the treatment of various inflammatory diseases [16][17][18][19] , we hypothesized that MIL would ameliorate DNFB-induced contact dermatitis, because this allergic dermatitis also shows phenomena similar to general inflammatory responses [3][4][5] . When the surface of the skin is inflamed, cracked or raw, initial application of DNFB induces stinging or burning sensations.…”

Section: Introductionmentioning

confidence: 99%

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Effects of Magnetic Infrared Laser on Dinitrofluorobenzene-induced Contact Dermatitis in Mice

et al. 2011

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Abstract.[Purpose] The purpose of the present study was to investigate the effect of a magnetic infrared laser (MIL) on the 2, 4-dinitrofluorobenzene (DNFB)-induced contact dermatitis, type I allergic mouse model. [Subjects] Male ICR mice.[Methods] Contact dermatitis was induced by sensitization with dinitrophenyl-derivatized ovalbumin (DNP-OVA) and DNFB challenge as antigen. MIL radiation doses of 1.33, 2.66 and 6.65 J/cm 2 were given, once a day for 7 continuous days, to DNP-OVA sensitized mice and compared with 15 mg/kg dexamethasone (intraperitoneal treatment). The edematous changes and scratching behavior were measured together with ear histopathological observations. [Results] Immediately after DNFB challenge to the ear or paw of DNP-OVA sensitized mice, increases of ear and paw thicknesses and weights were detected as well as anterior ear skin (dermis to epidermis) thickness and paw scratching behavior increases. However, these DNFB-induced increases of edematous changes and scratching behavior were significantly and dose-dependently decreased by MIL irradiation.[Conclusion] The results obtained in this study suggest that MIL radiation has relatively favorable effects on allergic dermatitis, and MIL therapy may represent an alternative approach for the treatment of allergic cutaneous diseases.

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