Immunoglobulin E antibodies to milk proteins

Letter, B. K; Gery, Igal; Freier, S; Noah, Zehava L.; Davies, Michael A.

doi:10.1111/j.1365-2222.1971.tb00777.x

Cited by 35 publications

(11 citation statements)

References 26 publications

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“…Reaction of mast cell-bound reagins with cow's milk protein antigen would then result in in creased vascular permeability and possible deposit of immune complexes inducing a lo cal inflammation in the mucosa. The noted low frequency of specific IgE antibodies in patients with acute reactions, although in agreement with the findings of Kletter et al [14], is obscure, as these patients often pre sented typical manifestations of immediate type allergy. Sampling was done immediate ly before positive provocations and, there fore, antibody consumption is no explana tion for the negative findings.…”

Section: Discussionsupporting

confidence: 89%

“…Dif ferent serologic techniques have been ap plied extensively in clinical work, so far without facilitating the diagnosis apprecia bly [24], Usually the applied methods have quantified the levels of specific antibodies to cow's milk proteins belonging to the immu noglobulin classes IgG or IgM [6,12,19,20], while investigations of IgE and IgA an tibodies are sparse in cases of gastrointesti nal cow's milk protein intolerance [5,14]. Furthermore, it is usually not possible to es tablish, in the reports, the relation between time of sampling and exposition/elimination.…”

Section: Introductionmentioning

confidence: 99%

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Specific Antibodies in Infants with Gastrointestinal Intolerance to Cow’s Milk Protein

Sp¹,

Ahlstedt²,

La³

1978

Int Arch Allergy Immunol

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Antibodies of various immunoglobulin classes against cow’s milk proteins were studied in infants and children with cow’s milk protein intolerance, gluten-sensitive enteropathy and acute gastroenteritis. Their IgE, IgG, IgM and IgA antibody levels determined with the enzyme-linked immunosorbent assay (ELISA) and the IgE antibodies also determined with RAST, were compared with reference groups of children and adults. IgE, IgG or IgA antibodies against unseparated cow’s milk proteins, α-lactalbumin, β-lactoglobulin, α-casein and β-casein were present in many of the studied samples, but did not discriminate between the individuals with and without intolerance symptoms. As a group, the infants with late reactions to cow’s milk showed increased levels of IgE and IgG antibodies detected with the ELISA, while patients with gluten-sensitive enteropathy had significantly increased levels of IgG and IgA antibodies to cow’s milk proteins compared to the reference group. By combining the findings of antibody increases in various immunoglobulin classes, an individual discrimination could be reached. Thus, 8 of 9 of the patients with late reactions to cow’s milk had increased levels of IgE or IgG + IgA antibodies as compared to 3 of 22 in the reference group. Serodiagnosis with the ELISA may, therefore, be of some use in patients with a suspicion of cow’s milk protein intolerance.

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Section: Discussionsupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Specific Antibodies in Infants with Gastrointestinal Intolerance to Cow’s Milk Protein

Sp¹,

Ahlstedt²,

La³

1978

Int Arch Allergy Immunol

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“…Kletter et fl/. [1] did not detect any IgE antibodies to non-processed ^-lactoglobulin in the sera from infants below 1-year-old with suspected cow's milk allergy, while in the present study, IgE antibodies to cow's milk were found even in young infants. The findings of high levels of IgG antibodies against cow's milk proteins, especiallŷ -lactoglobulin in slowly appearing intolerance to cow's milk, suggest that these reactions to cow's milk may be mediated by immune complexes.…”

Section: Discussioncontrasting

confidence: 65%

Serum antibodies against native, processed and digested cow's milk proteins in children with cow's milk protein intolerance

Fällström¹,

Ahlstedt²,

Carlsson³

et al. 1986

Clin Experimental Allergy

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Summary Children with or without cow's milk protein intolerance were investigated for serum antibodies to native cow's milk proteins, processed cow's milk proteins and native‐ and pepsin‐digested β‐lactoglobulin. Antibodies of IgG and IgA isotypes were determined with ELISA and those of IgE isotype with RAST. Children who exhibited slowly appearing untoward reactions to cow's milk feeding had significantly higher titres of IgG antibodies against both native and digested β‐lactoglobulin than children with an immediate type of reaction or no intolerance. The IgG antibodies to pepsin‐digested β‐lactoglobulin were efficiently inhibited by native β‐lactoglobulin even at low concentrations, which suggests that there were no antibodies specific for the degraded proteins. The children with slow reactions to cow's milk also tended to have higher antibody levels of the IgG and IgA isotypes to both native and processed cow's milk. Antibodies to this mix of antigens discriminated less well, however, than the antibodies to isolated β‐lactoglobulin between the groups of children with slowly appearing reactions, with immediate reactions and the controls. Seven out of nine children with an immediate type of reaction to cow's milk protein had IgE antibodies against cow's milk protein determined with the RAST, while this type of antibodies could not be demonstrated in any child in the two other groups. Using processed or digested protein as antigen did not increase the sensitivity of the antibody determinations.

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“…However, the circulating antibodies may participate in the disposal of environmental antigens from the gastrointestinal tract. Titers of antibodies to dietary antigens appear to decline with age during childhood, except in children with altered gastrointestinal perme ability [6], A few studies on the immunoglobulin class distri bution of antibodies against dietary antigens in chil dren have been published, and IgE antibodies have been implicated in food intolerance [7][8][9][10], Several aspects of the human immune response to dietary antigens such as its relation to food allergy, the specificity and avidity of the antibodies, and their isotype and subclass distribution require further studies [ 11],…”

Section: Introductionmentioning

confidence: 99%

Humoral Immunity to Dietary Antigens in Healthy Adults

Husby

Oxelius

Teisner

et al. 1985

Int Arch Allergy Immunol

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The occurrence of antibodies to five dietary protein antigens in the sera from 21 healthy adults was investigated by a modified Farr assay. Antibody to ovalbumin (OA) occurred most frequently (90%) whereas only 24% had antibodies to α-lactalbumin (ALA). No correlation was noted between the titer of antibodies against bovine serum albumin (BSA) and OA in the single individual. The avidity constants (10⁸––10⁹ l/mol) and cross-reactivities against other albumins of anti-BSA antibodies in two human sera were comparable to that of the antibodies in pooled hyperimmune rabbit antiserum. Crossed radioimmunoelectrophoresis showed serum anti-BSA and anti-OA antibodies to be predominantly of the IgG class (13/13, 10/10), occasionally of the IgA- (6/13, 1/10) and rarely of the IgM class (1/13, 0/10). Analysis by radioelectroimmunoassay (rocket immunoelectrophoresis) of the IgG subclass distribution of anti-BSA and anti-OA antibodies showed total absence of IgG3. In contrast, antibodies of the IgG4 subclass were frequently present even in sera with very low levels of total IgG4.

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Immunoglobulin E antibodies to milk proteins

Cited by 35 publications

References 26 publications

Specific Antibodies in Infants with Gastrointestinal Intolerance to Cow’s Milk Protein

Specific Antibodies in Infants with Gastrointestinal Intolerance to Cow’s Milk Protein

Serum antibodies against native, processed and digested cow's milk proteins in children with cow's milk protein intolerance

Humoral Immunity to Dietary Antigens in Healthy Adults

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