Immunoglobulin classes in plasma cells of the synovial membrane in chronic inflammatory joint disease.

Revell, Peter A.; Mayston, V

doi:10.1136/ard.45.5.405

Cited by 9 publications

(8 citation statements)

References 8 publications

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“…We confirmed the presence of B lymphocytes in psoriatic synovium, as has been shown previously [39]. Plasma cells were detected in few cases in the current study, in contrast to the findings of others [33]. Most cells were of the IgG isotype subset, suggesting that antigen-driven B-cell maturation with class switching to IgG had taken place.…”

Section: Immunophenotype Of the Inflammatory Cellssupporting

confidence: 93%

Inflammatory infiltrate and interleukin-8 expression in the synovium of psoriatic arthritis - an immunohistochemical and mRNA analysis

König¹,

Krenn

Gillitzer

et al. 1997

Rheumatology International

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Psoriatic arthritis is an inflammatory arthropathy that ultimately can lead to joint destruction. In this study, we investigated the immunophenotypes of the inflammatory cells and the expression of interleukin-8 (IL-8), which is the hallmark chemoattractant cytokine of psoriasis in synovial membranes from patients exhibiting active psoriatic synovitis (n = 9). The tissue samples were examined by immunohistochemistry, Western blot analysis and in situ hybridisation. The inflammatory infiltrate consisted predominantly of CD3+ T lymphocytes, with a higher proportion of CD4+ than CD8+ T lymphocytes in six cases. CD3+ T lymphocytes were focally distributed near small blood vessels and the enlarged synovial intima. CD1+ interdigitating reticulum cells were not detected. CD22+ B lymphocytes and plasma cells were found in small aggregates without KiM4+ follicular dendritic cells. KiM8+ macrophages were located in the synovial intima and were distributed in a diffuse pattern near the synovial lining cells. CD15+ neutrophil granulocytes were detected in four cases. They were preferentially located in the vicinity of blood vessels and the synovial intima. IL-8 was found at a high level in the synovial lining cells and to a lesser extent in cells located in the perivascular areas. Immunofluorescence double staining showed IL-8 to be expressed in KiM8+ multinucleated giant cells, KiM8+ macrophages and CD3+ T lymphocytes. IL-8 receptor A was demonstrated in the synovial lining and in macrophages and lymphocytes. IL-8 was detected by immunoblot analysis of the synovial tissue at 8.4 kD. Employing in situ hybridisation, IL-8 mRNA was strongly and preferentially expressed in the synovial intima, as well as in macrophages and lymphocytes. The immunophenotype of the psoriatic arthritis inflammatory cells shows great similarity to the inflammatory infiltrate found in the synovial tissue of patients with rheumatoid arthritis. The preferential expression of IL-8 and IL-8 mRNA in the enlarged synovial intima and in lymphocytes and macrophages suggests that IL-8 exerts its action through activated mononuclear cells and T lymphocytes. It seems to play a role in regulating leucocyte traffic into the enlarged synovial intima and may contribute to the aggressive synovitis of patients with psoriatic arthritis.

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Section: Immunophenotype Of the Inflammatory Cellssupporting

confidence: 93%

Inflammatory infiltrate and interleukin-8 expression in the synovium of psoriatic arthritis - an immunohistochemical and mRNA analysis

König¹,

Krenn

Gillitzer

et al. 1997

Rheumatology International

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“…All eight IgM hybridomas were derived from the membrane of the seropositive RA patient. This is consistent with findings that IgM plasma cells are rarely seen in synovial membranes of seronegative patients (Munthe & Natvig, 1972;Revell & Mayston, 1986), We believe that this report is the first report of both IgG-and IgM-secreting hybridomas derived from synovium, and represents an advance in the investigation of B cell function in RA, IgGl was the predominant IgG subclass secreted by 100% (17/17) and 89% (8/9) of the hybridomas from two separate fusions. This confirms the immunohistochemical findings of Mellbye et al (1984) who reported that the majority of plasma cells in 15 synovia from seropositive and eight synovia from seronegative RA patients were secreting IgGI.…”

Section: Discussionsupporting

confidence: 77%

Analysis of immunoglobulins secreted by hybridomas derived from rheumatoid synovia

Brown

Plater-Zyberk

Mageed

et al. 1990

Clinical and Experimental Immunology

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SUMMARYTwenty-six IgG-secreting and eight IgM-secreting hybridomas were derived from the synovia of two patients with rheumatoid arthritis (RA), Hybridomas were obtained by fusing a heteromyeloma cell line, SPAZ-4 with synovial mononuclear cells that were not deliberately stimulated in vitro. Over 96% of the IgG-seereting hybridomas produced antibodies which belonged to the IgGl subclass and showed lambda light chain predominance; the latter was not seen in IgM antibodies, where kappa light chains dominated by 3; 1, All IgG antibodies were eationie, Synovial B cells were not exposed to extrinsic stimuli prior to fusion, therefore these results refieet the state of B cell activation and differentiation in vivo. Our results indicate that IgG-secreting B eells in the RA joint are under a selective influence which is, as yet, unidentified. One out of eight IgM-seereting and two out of 26 IgG-secreting hybridomas produeed rheumatoid factors (RF), The IgM-RF specificity for IgG heavy chain subclasses was determined and showed that the monoclonal bound to IgG 1, IgG2 and IgG4 but not IgG3 with exception of IgG3 Goe ofthe G3m (st) allotype, a profile typical of specificity for the Ga epitope. This monoclonal also distinguished a determinant in the Fc region of human IgG which was not present in rabbit IgG, The overall frequency of RF-secreting hybridomas we observed indicates that B cells committed to RF production in the synovium of a seropositive and a seronegative RA patient is below 10%, Keywords human hybridomas synovial tissue rheumatoid arthritis immunoglobulin isotypes

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“…As expected for the normal adult Ig repertoire (30), JH4 turned out to be the most widely used J-gene segment, even though all other five known human JH gene segments were found, too.…”

Section: Results N Histopathology and Immunohistochemistry Of Pa Synomentioning

confidence: 93%

“…Psoriatic arthritis occurs in about 5-7% of patients showing the characteristic psoriatic skin lesions (26). The lymphocytic infiltrate of psoriatic synovitis consists predominantly of CD3 + T-lymphocytes (7,39) and of a variable but noticeable number of CD20 + B-lymphocytes and plasma cells, predominantly of the IgG and IgM isotypes (17,25,30).…”

Section: Introductionmentioning

confidence: 99%

IgVH-genes analysis from psoriatic arthritis shows involvement of antigen-activated synovial B-lymphocytes

Gerhard

Krenn

Magalhães

et al. 2002

Zeitschrift f�r Rheumatologie

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The pathogenesis of psoriatic arthritis (PA), which occurs in 5-7% of patients with psoriasis vulgaris, is enigmatic. There are no molecular data about synovial B-cells of chronic synovitis of PA. In order to understand the B-cell response in PA, we analysed IgVH genes and specified replacement to silent mutation (R/S) ratios of the complementarity determining regions (CDR) and framework regions (FR) as well as VH families. To prove the existence of a common pattern we additionally analysed the IgVH genes at the amino-acid level. From 5 PA patients we took cryo-tissue sections with somatically mutated IgVH genes (sum R/sum S in the CDRs: 2.5-6.8), 62 of which were analysed. In one patient two cases of clonally related IgVH genes were observed. Identical amino-acid replacements in IgVH1 and IgVH4 were found at the same mutational "cold spot". These data indicate that antigen-activated B-cells participate in the formation of chronic synovitis of PA. Since, neither histopathologically nor immunohistochemically, no germinal centres could be detected, the clonally related IgVH genes may be interpreted as residues of a germinal centre reaction that occurred before synovectomy. The existence of identical amino-acid replacement mutations in IgVH1 and IgVH4 genes suggests that a limited number of antigens are common to all PA patients analysed. The recombinant expression of the IgVH could help to define B-cell specificities underlying the pathogenesis of chronic synovitis and dermatitis of PA.

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Immunoglobulin classes in plasma cells of the synovial membrane in chronic inflammatory joint disease.

Cited by 9 publications

References 8 publications

Inflammatory infiltrate and interleukin-8 expression in the synovium of psoriatic arthritis - an immunohistochemical and mRNA analysis

Inflammatory infiltrate and interleukin-8 expression in the synovium of psoriatic arthritis - an immunohistochemical and mRNA analysis

Analysis of immunoglobulins secreted by hybridomas derived from rheumatoid synovia

IgVH-genes analysis from psoriatic arthritis shows involvement of antigen-activated synovial B-lymphocytes

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