Immunoglobulin Class Distribution of Measles Virus Antibodies in Serum and Spinal Fluids of Patients with Subacute Sclerosing Panencephalitis

Luster, Michael I.; Armen, Robert C.; Hallum, Jules V.; Ahmed, Akhtar; Leslie, Gerrie A.

doi:10.1159/000232063

Cited by 12 publications

(4 citation statements)

References 7 publications

(11 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We presume that the increase in that complement fraction is a response to a prolonged inflammatory process (19). A higher level of serum IgG has already been observed in patients with SSPE (5,20). Increased synthesis of that immunoglobulin indicates its active involvement in the immunological response to measles infection.…”

Section: Discussionmentioning

confidence: 80%

Subacute sclerosing panencephalitis: influence of the clinical course and treatment with isoprinosine on non-specific cell-mediated and humoral immunity

Vainiene

Członkowska

Korlak

1983

Acta Neurologica Scandinavica

View full text Add to dashboard Cite

Cell-mediated and humoral immunity were studied in 30 patients with subacute sclerosing panencephalitis (SSPE). Marked changes in cell-mediated immunity were observed, manifested as a decrease in total lymphocyte count, decrease of proportion of T cells forming late E rosettes, depression in lymphocyte blastogenesis, production of migration inhibition factor and delayed-type skin reactivity. The humoral immune responses was not so markedly changed and only increased levels of serum IgG, IgM and the C3c fragment of complement were noticed. The changes in immunity were more pronounced in patients with a more advanced stage of the disease. 19 patients were investigated 3 times at 7-week intervals during treatment with isoprinosine. In the group of patients (10 cases) whose clinical status was rapidly deteriorating, a marked decline of cell-mediated immunity was observed. In the group of patients showing a stationary course of the disease, cellular immunity was improving. The mechanism of the disturbances of non-specific immunity in SSPE and the influence of isoprinosine on the immune answer are discussed.

show abstract

Section: Discussionmentioning

confidence: 80%

Subacute sclerosing panencephalitis: influence of the clinical course and treatment with isoprinosine on non-specific cell-mediated and humoral immunity

Vainiene

Członkowska

Korlak

1983

Acta Neurologica Scandinavica

View full text Add to dashboard Cite

show abstract

“…Whether patients with subacute sclerosing panencephalitis (SSPE) have prolonged measles virus (MV) IgM antibody synthesis is a question that has been Accepted for publication May 21, 1985. investigated by several laboratories. However, a unanimous answer to this question has not been reached, because MV IgM synthesis has been reported in none [Najera et al, 1972;Massaro et al, 19781, in some [Thomson et al, 1975;Mehta et al, 1977;Luster et al, 1978;Ziola et al, 19791, and in all of the SSPE patients studied [Connolly et al, 1971;Chin-Mei and Szu-Chi, 1977;Kiessling et al, 19771. These disparate results could be due to one or more of several factors, including small numbers of patients, low sensitivity of the MV IgM antibody assay used, and not having taken into account possible interference by IgM-class rheumatoid factor (RF) when a MV IgM immunoassay was used.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of measles virus‐specific IgM antibodies and IgM‐class rheumatoid factor in relation to clinical onset of subacute sclerosing panencephalitis

Ziola

Halonen

Enders³

1986

Journal of Medical Virology

View full text Add to dashboard Cite

Thirty-seven serum and 37 cerebrospinal fluid (CSF) specimens from 27 patients with subacute sclerosing panencephalitis (SSPE) were tested for measles virus (MV) IgM antibodies and IgM-class rheumatoid factor (RF) to determine if a temporal relationship exists between SSPE onset and these immunoglobulins. Sensitive solid-phase radioimmunoassays were used for immunoglobulin detection. Five of six MV IgM-positive CSF, both MV IgM-positive sera and six out of eight sera with elevated RF levels were collected within 1 yr of SSPE onset. Also collected during this time were six other sera having high binding in the MV IgM assay, which was not due to MV-specific IgM antibodies. Two of these sera had as high or higher binding of IgM to a Vero cell control antigen, suggesting involvement of an IgM-class autoantibody. The other four sera had false-positive MV IgM assay results due to RF interference. RF interference was dependent on both the titer and avidity of the MV IgG antibodies involved. Three conclusions can be drawn from these results. First, MV IgM antibodies and elevated RF levels are not markers for acute SSPE, despite the tendency for their synthesis at this stage of the disease. Second, immunoassay analysis of viral IgM antibodies must employ an appropriate control antigen to account for background IgM binding. Finally, even if RF levels are normal or near normal, a false-positive IgM immunoassay result can still occur if antigen-specific IgG antibodies in the same sample have the right combination of titer and avidity.

show abstract

Section: Introductionmentioning

confidence: 99%

“…Measles-virus-specific IgM antibodies have been repeatedly detected in sera and CSF from patients with SSPE [Arstila et al, 1977;Connolly et al, 1971;Kiessling et al, 1977;Luster et al, 19781. In patients with MS the documentation of the presence of IgM antibodies against measles virus antigens is more diffuse [Haire et al, 1973;Millar et al, 19711. Specific IgM antibodies have been demonstrated in sera from patients with an acute measles virus infection at the time of appearance of the rash and up to 1-3 months later [Arstila et al, 1977;Tuokko and Salmi, 1983;Vuorimaa et al, 19781.…”

Section: Introductionmentioning

confidence: 99%

IgM antibodies in sera from patients with chronic active hepatitis reacting with the measles virus matrix protein and nucleoprotein

Christie

Endresen

Haukenes

1984

Journal of Medical Virology

View full text Add to dashboard Cite

The antigenic specificity of measles virus IgM antibodies in sera from patients with chronic active hepatitis not caused by hepatitis B virus has been examined. An immunosorbent column containing antihuman IgM covalently bound to Sepharose was used to pick up IgM from the sera. Radiolabelled measles virus antigens were then allowed to react with the IgM antibodies. The immune complexes were eluted and analysed by sodium dodecyl sulfate [SDS]-polyacrylamide gel electrophoresis. Four sera from patients with hepatitis B surface antigen [HBsAg]-negative chronic active hepatitis with high measles virus haemagglutination inhibition [HI] and complement fixation [CF] antibody titres and positive enzyme-linked immunosorbent assay [ELISA] for measles-virus-specific IgM were examined. The results were compared with those obtained using sera from patients with an acute measles virus infection and from healthy controls. In both patient groups, IgM antibodies with specificity against the matrix protein represented the major portion of the measles virus IgM. IgM antibodies against the measles virus nucleoprotein and probably against host-cell-derived actin were also present. The patient sera contained only traces of IgM antibodies with specificity against the measles virus haemagglutinin or fusion protein. No specific IgM antibodies were found in sera from healthy controls.

show abstract

Immunoglobulin Class Distribution of Measles Virus Antibodies in Serum and Spinal Fluids of Patients with Subacute Sclerosing Panencephalitis

Cited by 12 publications

References 7 publications

Subacute sclerosing panencephalitis: influence of the clinical course and treatment with isoprinosine on non-specific cell-mediated and humoral immunity

Subacute sclerosing panencephalitis: influence of the clinical course and treatment with isoprinosine on non-specific cell-mediated and humoral immunity

Synthesis of measles virus‐specific IgM antibodies and IgM‐class rheumatoid factor in relation to clinical onset of subacute sclerosing panencephalitis

IgM antibodies in sera from patients with chronic active hepatitis reacting with the measles virus matrix protein and nucleoprotein

Contact Info

Product

Resources

About