Immunoglobulin Analysis of C57BL Mice Infected With a Radiation-Induced Leukemia Virus 2

Assay of tetramisole in immune-depressed mice

“…As described previously (Sassen et al, 1974), the RadLV(D)-inoculated mice show an increasing serum level of immunoglobulin followed by a plateau and then a decrease which occurs shortly before death. Consequently, a more pronounced increase of immunoglobulin during Tetramisole therapy cannot be regarded as protection.…”

“…Since immune depression is supposedly needed to see an effect of the drug, there could, perhaps, be a slight doubt as to the existence of such a deficiency during the leukemia experiments and, especially, during the rather long latent period of the induced radiation leukemia. In fact, humoral immunodepression has been found during the RadLV(D) disease (Sassen et al, 1974), and administration of tile Rauscher virus produced a decrease of humoral antibodies as well as a depressed cell-mediated immunity (Mortensen et al, 1973). Moreover, shortly after whole-body irradiation, immunological defects of both thymus and bone-marrow-derived lymphocytes have been sufficiently demonstrated (Gottlieb and Makinodan, 1974).…”

“…The first is a reticulum cell sarcoma produced by a virus called RadLV(D), which originated from irradiated C57B1 mice (Mistry and Duplan, 1973). The RadLV(D) disease is accompanied by progressive spleen and lymph node hypertrophy, increased immunoglobulin level, decreased humoral immune response and death occurring 3-6 months after injection of the viral extract (Sassen et al, 1974).…”

“…The antiserum, control values and qualitative analyses have been previously described (Sassen et al, 1974). Each serum was tested in duplicate.…”

Mice transgenic for a soluble form of murine cytotoxic T lymphocyte antigen 4 are refractory to murine acquired immune deficiency sydrome development

“…B cell activation occurring in MAIDS is associated with hypergammaglobulinaemia 2 , 21 . Accordingly, the serum levels of IgG2a, IgG3 and IgM quantified by ELISA, were found to be several fold increased in Du5H(G6T2)‐inoculated non‐transgenic mice (Table 2).…”