Immunoglobulin A, G, and M responses to L1 and L2 capsids of human papillomavirus types 6, 11, 16, 18, and 33 L1 after newly acquired infection.

Abstract

Objectives: We performed a study to establish the pattern of serological reactivity for immunoglobulins (Ig), to capsids of human papilloma virus (HPV) after new HPV infection in two groups of subjects. Methods: The pattern of serological reactivity after acquisition of infection with HPV was investigated by measuring IgA, IgM, and IgG antibodies to capsids containing L1 and L2 proteins of HPV types 6, 11, 16, 18, and 33 in longitudinal studies of groups with diVerent patterns of sexual activity. Individuals who tested negative for HPV DNA by the polymerase chain reaction at enrolment, but who became HPV DNA positive during follow up, were examined for antibodies to HPV capsids by enzyme linked immunosorbent assay. One group consisted of 15 young girls (with eight controls who remained HPV DNA negative) who were becoming sexually active and the other comprised 12 male (with five controls) and 35 female (with seven controls) heterosexual attenders of a sexually transmitted disease clinic who had had multiple sexual partners. Results: The sexually inexperienced girls showed IgA and IgG responses, but seldom an IgM response to infection with HPV types 6/11, 16, and 18. No consistent pattern of serological reactivity was apparent for the heterosexuals with multiple partners. The lack of association between current HPV DNA positivity and detectable antibodies in these individuals was possibly related to the duration of infection or to prior exposure to HPV. For the latter group serological reactivity to HPV capsids was significantly greater in women than in men (p =0.001, p = 0.003, and p = 0.024, for IgG to HPV 6, 11, and 16, respectively). Conclusion:The sex diVerence in antibody response detected in previous studies with assays based on peptide antigens was thus corroborated in the present study with capsid based serological assays. This sex diVerence might reflect a diVerence in sexual activity and prior exposure to HPV between men and women in this particular group. (Sex Transm Inf 1998;74:354-360)

Determinants of human papillomavirus 16 serological conversion and persistence in a population-based cohort of 10 000 women in Costa Rica

“…being seropositive) remains unclear (Stone et al, 2002). Reported to be high over a long period of time (van Doornum et al, 1998), serology measures are likely to be confounded by repeated exposure to HPV-16 (Onda et al, 2003). Persistence of HPV-16 antibodies has been found for 7 -13 years (Shah et al, 1997) and for 4 years in a study of 1656 pregnant women who demonstrated stable antibody levels (af Geijersstam et al, 1998).…”

Reactivity pattern of 92 monoclonal antibodies with 15 human papillomavirus types

“…Upon infection with HPV, serum antibodies to L1 protein can develop (Dillner, 1999;van Doornum et al, 1998;Wang et al, 1996;Wideroff et al, 1999). This immune response is highly type-specific (Carter et al, 1996(Carter et al, , 2000Giroglou et al, 2001;Wang et al, 1997) and persists for years (Carter et al, 2000;Dillner, 1999).…”

Human Papillomavirus-Specific Antibody Status in Oral Fluids Modestly Reflects Serum Status in Human Immunodeficiency Virus-Positive Individuals

“…Oropositivity was defined by an adj OD value greater than 2 standard deviations above the mean of the adj OD values of the children's oral samples, with those children's samples falling above this cutpoint being excluded and the cutpoint being recalculated (4,22,34,35,37,54). When no negative control population was available, arbitrarily selected cutpoints were used to determine oropositivity (7,11,14,49,53). Other approaches for determining assay cutpoints for oral specimens were examined, including mathematical modeling, with the assumption that the adj OD values would fall into two groups, defined as oropositive and oronegative, and a modified receiver operator curve approach (23,40,47).…”