Immunogens for the preparation of retinoic acid antibodies

Zhou, Hui Ren; Cullum, Malford E.; Gerlach, Thomas; Gage, Douglas A.; Zile, Maija H.

doi:10.1016/0955-2863(91)90002-m

Cited by 6 publications

(3 citation statements)

References 23 publications

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“…Synthesis of the Retinoic Acid − HRP Conjugate. The method of synthesis (Figure ) was modified starting with a procedure reported earlier . Retinoic acid, 0.1 mmol, was dissolved in 2.4 mL of dimethyl formamide (DMF).…”

Section: Methodsmentioning

confidence: 99%

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Thermometric Sensing of Peroxide in Organic Media. Application To Monitor the Stability of RBP−Retinol−HRP Complex

2000

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The stability of horseradish peroxidase (HRP) in aqueous and organic solvents is applied to develop a simple thermometric procedure to detect the binding of retinoic acid-HRP conjugate to retinol binding protein (RBP). Butanone peroxide (BP) in organic phase and hydrogen peroxide in aqueous phase is detected thermometrically on a HRP column, immobilized by cross-linking with glutaraldehyde on controlled pore glass (CPG). Acetone, acetonitrile, methanol, and 2-butanol are used for detection of BP, in the flow injection analysis (FIA) mode. A linear range between 1 and 50 mM BP is obtained in all the organic solvents with a precision of 5-7% (CV%). The magnitude and nature of the thermometric response is significantly different in each organic solvent. The stability of HRP in the organic phase is used to study the stability of a retinoic acid-HRP conjugate bound to immobilized RBP. The response of HRP (to 20 mM BP) in the retinoic acid-HRP conjugate is used as an indicator of the stability of the RBP-retinoic acid-HRP complex, after challenges with various organic/aqueous solvents. Both immobilized HRP and RBP are stable at least for 6 months. The effect of o-phenylene diamine on the thermometric response of HRP is also investigated. A scheme for the design of a thermometric retinol (vitamin A) biosensor is proposed.

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Section: Methodsmentioning

confidence: 99%

“…The method of synthesis (Figure 1) was modified starting with a procedure reported earlier. 23 Retinoic acid, 0.1 mmol, was dissolved in 2.4 mL of dimethyl formamide (DMF). Similarly, 0.1 mmol of NHS and 0.1 mmol of dicyclohexyl carbodiimide (DCC) were dissolved in 0.1 mL of DMF separately.…”

Section: Methodsmentioning

confidence: 99%

Thermometric Sensing of Peroxide in Organic Media. Application To Monitor the Stability of RBP−Retinol−HRP Complex

2000

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“…Some authors demonstrated that the binding BSA–flavonoid did not affect either stability or the antioxidant activity of the conjugate . Using the BSA–conjugate technique, many reports have shown the preparation of antibodies against nutrients or their metabolites . In terms of the flavonoids, few authors have developed antibodies against tri‐ and tetramethoxy‐flavone glycosides and then used them for pharmacokinetic studies …”

Section: Introductionmentioning

confidence: 99%