Immunogenomic analysis of insect bite hypersensitivity in a model horse population

Vychodilova, Leona; Matiašovic, Ján; Bobrova, Olga; Futas, Ján; Klumplerová, Marie; Stejskalová, Karla; Cvanová, Michaela; Jánová, Eva; Osičková, Jitka; Vyskočil, M.; Sedlinská, Markéta; Dušek, Ladislav; Marti, Eliane; Hořín, Petr

doi:10.1016/j.vetimm.2012.12.013

Cited by 21 publications

(14 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The observation that environmental factors can reduce the incidence of allergic disease is similar to that posited by parts of the hygiene hypothesis in human allergic disease (72). Notwithstanding these observations which strongly support environmental factors as important in the aetiology of IBH, several studies have also demonstrated genetic factors related to the development of IBH (73)(74)(75)(76)(77). The extent to which genetic predisposition can be overridden by immunological or environmental factors is important; as this will have a bearing on the efficacy of future therapeutic measures.…”

Section: Insect Bite Hypersensitivitysupporting

confidence: 56%

Immune responses to ectoparasites of horses, with a focus on insect bite hypersensitivity

Wilson

2014

Parasite Immunology

View full text Add to dashboard Cite

Horses are affected by a wide variety of arthropod ectoparasites, ranging from lice which spend their entire life on the host, through ticks which feed over a period of days, to numerous biting insects that only transiently visit the host to feed. The presence of ectoparasites elicits a number of host responses including innate inflammatory responses, adaptive immune reactions and altered behaviour; all of which can reduce the severity of the parasite burden. All of these different responses are linked through immune mechanisms mediated by mast cells and IgE antibodies which have an important role in host resistance to ectoparasites, yet immune responses also cause severe pathological reactions. One of the best described examples of such pathological sequelae is insect bite hypersensitivity (IBH) of horses; an IgE-mediated type 1 hypersensitivity to the salivary proteins of Culicoides spp. associated with T-helper-2 production of IL4 and IL13. Importantly, all horses exposed to Culicoides have an expanded population of Culicoides antigen-specific T cells with this pattern of cytokine production, but in those which remain healthy, the inflammatory reaction is tempered by the presence of FoxP3+ CD4+ regulatory T cells that express IL10 and TGF-beta, which suppresses the IL4 production by Culicoides antigen-activated T cells.

show abstract

Section: Insect Bite Hypersensitivitysupporting

confidence: 56%

Immune responses to ectoparasites of horses, with a focus on insect bite hypersensitivity

Wilson

2014

Parasite Immunology

View full text Add to dashboard Cite

show abstract

“…Moreover, we found a trend toward higher levels of TSLP and MCP-1 in IBH lesions compared to healthy skin, confirming earlier suggested data. 18,22,23 Insect bite hypersensitivity is defined as type I allergy with type IVb characteristics. [18][19][20][21] Type I pathology includes IgE-mediated mast cell and basophil activation when allergen-specific IgE binds to its Fc ε receptor type I (FcεRI), leading to histamine release from cellular granules.…”

Section: Discussionmentioning

confidence: 99%

“…were associated with IBH disease pathology. 18,22,23 The role of IL-31 in pruritic IBH lesions of the horse remains elusive. Similar to our earlier described therapeutic vaccine targeting equine IL-5 (eIL-5) in order to reduce eosinophil production and infiltration into the skin, we developed a therapeutic vaccine targeting equine IL-31 (eIL-31).…”

mentioning

confidence: 99%

Interleukin 31 in insect bite hypersensitivity—Alleviating clinical symptoms by active vaccination against itch

Olomski

Fettelschoss

Jónsdóttir

et al. 2020

Allergy

Self Cite

View full text Add to dashboard Cite

Background Insect bite hypersensitivity (IBH) is the most common seasonal pruritic allergic dermatitis of horses occurring upon insect bites. In recent years, a major role for IL‐31 in allergic pruritus of humans, monkeys, dogs, and mice was acknowledged. Here, we investigate the role of IL‐31 in IBH of horses and developed a therapeutic vaccine against equine IL‐31 (eIL‐31). Methods IL‐31 levels were quantified in allergen‐stimulated peripheral blood mononuclear cells (PBMCs) and skin punch biopsies of IBH lesions and healthy skin from IBH‐affected and healthy horses. The vaccine consisted of eIL‐31 covalently coupled to a virus‐like particle (VLP) derived from cucumber mosaic virus containing a tetanus toxoid universal T‐cell epitope (CuMVTT). Eighteen IBH‐affected horses were recruited and immunized with 300 μg of eIL‐31‐CuMVTT vaccine or placebo and IBH severity score was recorded. Results IL‐31 was increased in PBMCs and exclusively detectable in skin lesions of IBH‐affected horses. Vaccination against eIL‐31 reduced delta clinical scores when compared to previous untreated IBH season of the same horses and to placebo‐treated horses in the same year. The vaccine was well tolerated without safety concerns throughout the study. Conclusion TH2‐derived IL‐31 is involved in IBH pathology and accordingly the immunotherapeutic vaccination approach targeting IL‐31 alleviated clinical scores in affected horses.

show abstract

“…A few candidate gene studies or genome‐wide association (GWA) studies of IBH susceptibility in horses have previously been published. The equine leucocyte antigen (ELA) class II region (Andersson et al, ; Klumplerova et al, ), as well as some non‐ELA genes regulating immunity and allergy (Klumplerova et al, ; Vychodilova et al, ), was suggested in candidate gene studies to influence IBH susceptibility. In a study on Icelandic horses and Exmoor ponies (Andersson et al, ), the same microsatellite marker in the ELA class II region was found to be associated with IBH susceptibility in both breeds, indicating that the genetic background of IBH susceptibility may be at least partly similar in these breeds.…”

Section: Introductionmentioning

confidence: 99%

Genome‐wide association study for insect bite hypersensitivity susceptibility in horses revealed novel associated loci on chromosome 1

Shrestha

Solé

Ducro

et al. 2019

J Animal Breeding Genetics

View full text Add to dashboard Cite

Equine insect bite hypersensitivity (IBH) is a pruritic skin allergy caused primarily by biting midges, Culicoides spp. IBH susceptibility has polygenic inheritance and occurs at high frequencies in several horse breeds worldwide, causing increased costs and reduced welfare of affected horses. The aim of this study was to identify and validate single nucleotide polymorphisms (SNPs) associated with equine IBH susceptibility. After quality control, 33,523 SNPs were included in a Bayesian genome‐wide association study on 177 affected and 178 unaffected Icelandic horses. We report associated regions in E. caballus (ECA) 1, 3, 15 and 18, overlapping with known IBH QTLs in horses, and novel regions containing several genes, together explaining 11.46% of the total genetic variance. For validation, three SNPs on ECA 1 and ECA X (explaining the largest percentage of genetic variance) within 1‐mb genomic windows for IBH were genotyped in an independent population of 280 Exmoor ponies. The associated genomic region (152–153 mb) on ECA 1 was confirmed in Exmoor ponies and contains the AQR gene involved in splicing processes and a long non‐coding RNA. This study confirms the polygenic nature of IBH susceptibility and suggests a role of transcriptional regulatory mechanisms (e.g., alternative splicing) for IBH predisposition in these horse breeds.

show abstract

Immunogenomic analysis of insect bite hypersensitivity in a model horse population

Cited by 21 publications

References 42 publications

Immune responses to ectoparasites of horses, with a focus on insect bite hypersensitivity

Immune responses to ectoparasites of horses, with a focus on insect bite hypersensitivity

Interleukin 31 in insect bite hypersensitivity—Alleviating clinical symptoms by active vaccination against itch

Genome‐wide association study for insect bite hypersensitivity susceptibility in horses revealed novel associated loci on chromosome 1

Contact Info

Product

Resources

About