Immunogenicity Studies in Carnivores Using a Rabies Virus Construct with a Site-Directed Deletion in the Phosphoprotein

Vos, Ad; Conzelmann, Karl‐Klaus; Finke, Stefan; Müller, Thomas; Teifke, Jens Peter; Fooks, Anthony R.; Neubert, Andreas

doi:10.4061/2011/898171

Cited by 7 publications

(3 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These were obtained through a search on PubMed with the terms “rabies oral vaccination”, “rabies challenge studies in wildlife”. Of the 42 articles initially identified by description in the abstracts, 6 were removed for lack of serological data leaving 35 articles [ 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 ]. Six of the articles included studies on more than one species.…”

Section: Methodsmentioning

confidence: 99%

Rabies Virus Antibodies from Oral Vaccination as a Correlate of Protection against Lethal Infection in Wildlife

et al. 2017

Self Cite

View full text Add to dashboard Cite

Both cell-mediated and humoral immune effectors are important in combating rabies infection, although the humoral response receives greater attention regarding rabies prevention. The principle of preventive vaccination has been adopted for strategies of oral rabies vaccination (ORV) of wildlife reservoir populations for decades to control circulation of rabies virus in free-ranging hosts. There remains much debate about the levels of rabies antibodies (and the assays to measure them) that confer resistance to rabies virus. In this paper, data from published literature and our own unpublished animal studies on the induction of rabies binding and neutralizing antibodies following oral immunization of animals with live attenuated or recombinant rabies vaccines, are examined as correlates of protection against lethal rabies infection in captive challenge settings. Analysis of our studies suggests that, though serum neutralization test results are expected to reflect in vivo protection, the blocking enzyme linked immunosorbent assay (ELISA) result at Day 28 was a better predictor of survival. ELISA kits may have an advantage of greater precision and ability to compare results among different studies and laboratories based on the inherent standardization of the kit format. This paper examines current knowledge and study findings to guide meaningful interpretation of serology results in oral baiting monitoring.

show abstract

Section: Methodsmentioning

confidence: 99%

Rabies Virus Antibodies from Oral Vaccination as a Correlate of Protection against Lethal Infection in Wildlife

et al. 2017

Self Cite

View full text Add to dashboard Cite

show abstract

“…Importantly, such vaccines should not simply be “weakened”, but should have the potential to induce stronger immune responses that could generate protection more efficiently. This promise is yet to be realized, however, as a recent study using a P protein-mutated RABV strain unable to prevent IFN induction could induce protection in orally-vaccinated foxes, but gave no apparent improvement in protection compared with the wild-type strain [ 73 ]. The effectiveness of such mutations in attenuating strains optimized for immunogenicity, or of alternative strategies targeting STAT antagonism by P protein, or RIG-I-antagonism by N protein, awaits further research.…”

Section: Perspectivesmentioning

confidence: 99%

The importance of immune evasion in the pathogenesis of rabies virus

Ito

Moseley

Sugiyama

2016

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

Rabies is a zoonotic disease caused by the Lyssavirus rabies virus (RABV) that can infect most mammals, including humans, where it has a case-fatality rate of almost 100%. Although preventable by vaccination, rabies causes c. 59,000 human fatalities every year worldwide. Thus, there exists an urgent need to establish an effective therapy and/or improve dissemination of vaccines for humans and animals. These outcomes require greater understanding of the mechanisms of RABV pathogenesis to identify new molecular targets for the development of therapeutics and/or live vaccines with high levels of safety. Importantly, a number of studies in recent years have indicated that RABV specifically suppresses host immunity through diverse mechanisms and that this is a key process in pathogenicity. Here, we review current understanding of immune modulation by RABV, with an emphasis on its significance to pathogenicity and the potential exploitation of this knowledge to develop new vaccines and antivirals.

show abstract

“…RABVs expressing P that is unable to inhibit IRF3 activation are attenuated in wt mice, but not in IFNAR ko mice (Rieder et al, 2011). One of these viruses tested as a vaccine candidate successfully protected foxes from RABV challenge, although neutralizing antibody titers were lower than with standard vaccine; the same virus failed to induce sufficient levels of neutralizing antibodies and protection in skunks (Vos et al, 2011). RABV expressing a mutant form of P (W265G/M287V) unable to interact with STAT1 was completely attenuated in ddY mice (Wiltzer et al, 2014).…”

Section: Developing New Vaccines Against Ns-nsv: Rational Attenuationmentioning

confidence: 99%

Reverse genetics of Mononegavirales: How they work, new vaccines, and new cancer therapeutics

2015

View full text Add to dashboard Cite

The order Mononegavirales includes five families: Bornaviridae, Filoviridae, Nyamaviridae, Paramyxoviridae, and Rhabdoviridae. The genome of these viruses is one molecule of negative-sense single strand RNA coding for five to ten genes in a conserved order. The RNA is not infectious until packaged by the nucleocapsid protein and transcribed by the polymerase and co-factors. Reverse genetics approaches have answered fundamental questions about the biology of Mononegavirales. The lack of icosahedral symmetry and modular organization in the genome of these viruses has facilitated engineering of viruses expressing fluorescent proteins, and these fluorescent proteins have provided important insights about the molecular and cellular basis of tissue tropism and pathogenesis. Studies have assessed the relevance for virulence of different receptors and the interactions with cellular proteins governing the innate immune responses. Research has also analyzed the mechanisms of attenuation. Based on these findings, ongoing clinical trials are exploring new live attenuated vaccines and the use of viruses re-engineered as cancer therapeutics.

show abstract

Immunogenicity Studies in Carnivores Using a Rabies Virus Construct with a Site-Directed Deletion in the Phosphoprotein

Cited by 7 publications

References 32 publications

Rabies Virus Antibodies from Oral Vaccination as a Correlate of Protection against Lethal Infection in Wildlife

Rabies Virus Antibodies from Oral Vaccination as a Correlate of Protection against Lethal Infection in Wildlife

The importance of immune evasion in the pathogenesis of rabies virus

Reverse genetics of Mononegavirales: How they work, new vaccines, and new cancer therapeutics

Contact Info

Product

Resources

About