Immunogenicity of Vibrio cholerae outer membrane vesicles secreted at various environmental conditions

Adriani, Rezaei; Gargari, Seyed Latif Mousavi; Nazarian, Javad; Sarvary, Samaneh; Noroozi, Nafiseh

doi:10.1016/j.vaccine.2017.09.004

Cited by 26 publications

(17 citation statements)

References 46 publications

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“…However, the finding that OMVs play a crucial role in the pathogenesis of enteric infections has brought about a renewed interest and directed a new wave of research in this area. OMVs have been tested as antigens for vaccines that target pathogenic E. coli (119), Salmonella (120), Vibrio cholerae (121, 122), and combinations of pathogens (123). OMVs carry a complex mixture of biologically active molecules, including lipopolysaccharide, proteins, and phospholipids.…”

Section: Omv-host Cell Interactions In the Gastrointestinal Tractmentioning

confidence: 99%

Outer Membrane Vesicle-Host Cell Interactions

et al. 2019

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Outer membrane vesicles (OMVs) are nanosized proteoliposomes derived from the outer membrane of Gram-negative bacteria. They are ubiquitously produced both in culture and during infection, and are now recognized to play crucial roles during host-microbe interactions. OMVs can transport a broad range of chemically diverse cargoes, including lipids and lipopolysaccharides, membrane embedded and associated proteins and small molecules, peptidoglycan and nucleic acids. Particularly virulence factors such as adhesins and toxins are often enriched in OMVs. Here, we discuss a variety of ways in which OMVs facilitate host-microbe interactions, including their contributions to biofilm formation, nutrient scavenging, and modulation of host cell function. We particularly examine recent findings regarding outer membrane vesicle – host cell interactions in the oral cavity and the gastrointestinal tract.

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Section: Omv-host Cell Interactions In the Gastrointestinal Tractmentioning

confidence: 99%

Outer Membrane Vesicle-Host Cell Interactions

et al. 2019

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“…While this approach has yielded important basic information concerning their biochemical potential, it has recently become clear that the composition and components of OMVs can vary substantially based on the conditions in which they are produced (77)(78)(79)(80). In some cases, researchers have exploited this variation to isolate OMVs with enriched compositions more suited to a desired goal (81).…”

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confidence: 99%

Ambient pH Alters the Protein Content of Outer Membrane Vesicles, Driving Host Development in a Beneficial Symbiosis

et al. 2019

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Outer membrane vesicles (OMVs) are continuously produced by Gram-negative bacteria and are increasingly recognized as ubiquitous mediators of bacterial physiology. In particular, OMVs are powerful effectors in interorganismal interactions, driven largely by their molecular contents. These impacts have been studied extensively in bacterial pathogenesis but have not been well documented within the context of mutualism. Here, we examined the proteomic composition of OMVs from the marine bacterium Vibrio fischeri, which forms a specific mutualism with the Hawaiian bobtail squid, Euprymna scolopes. We found that V. fischeri upregulates transcription of its major outer membrane protein, OmpU, during growth at an acidic pH, which V. fischeri experiences when it transitions from its environmental reservoir to host tissues. We used comparative genomics and DNA pulldown analyses to search for regulators of ompU and found that differential expression of ompU is governed by the OmpR, H-NS, and ToxR proteins. This transcriptional control combines with nutritional conditions to govern OmpU levels in OMVs. Under a host-encountered acidic pH, V. fischeri OMVs become more potent stimulators of symbiotic host development in an OmpU-dependent manner. Finally, we found that symbiotic development could be stimulated by OMVs containing a homolog of OmpU from the pathogenic species Vibrio cholerae, connecting the role of a well-described virulence factor with a mutualistic element. This work explores the symbiotic effects of OMV variation, identifies regulatory machinery shared between pathogenic and mutualistic bacteria, and provides evidence of the role that OMVs play in animal-bacterium mutualism. IMPORTANCE Beneficial bacteria communicate with their hosts through a variety of means. These communications are often carried out by a combination of molecules that stimulate responses from the host and are necessary for development of the relationship between these organisms. Naturally produced bacterial outer membrane vesicles (OMVs) contain many of those molecules and can stimulate a wide range of responses from recipient organisms. Here, we describe how a marine bacterium, Vibrio fischeri, changes the makeup of its OMVs under conditions that it experiences as it goes from its free-living lifestyle to associating with its natural host, the Hawaiian bobtail squid. This work improves our understanding of how bacteria change their signaling profile as they begin to associate with their beneficial partner animals.

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“…Therefore, tailoring of the RPT tests, as for the case of the modified model used to test 1790GAHB, may be the appropriate way to predict reactogenicity of vaccine administered by a route different from the intramuscular; for example, it might be appropriate the use of pulmonary or oral routes of pyrogenicity testing in RPT for OMV-based vaccines that are envisaged to be administered via these routes in humans to induce optimal immunogenicity. 73,77…”

Section: Rabbit Pyrogenicity Testmentioning

confidence: 99%

Outer membrane vesicles: moving within the intricate labyrinth of assays that can predict risks of reactogenicity in humans

Rossi

Citiulo

Mancini

2020

Human Vaccines & Immunotherapeutics

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Outer membrane vesicles (OMV) are exosomes naturally released from the surface of Gram-negative bacteria. Since the '80s, OMVs have been proposed as powerful vaccine platforms due to their intrinsic self-adjuvanticity and ability to present multiple antigens in natural conformation. However, the presence of several pathogen-associated molecular patterns (PAMPs), especially lipid A, has raised concerns about potential systemic reactogenicity in humans. Recently, chemical and genetic approaches allowed to efficiently modulate the balance between reactogenicity and immunogenicity for the use of OMV in humans. Several assays (monocyte activation test, rabbit pyrogenicity test, limulus amebocyte lysate, human transfectant cells, and toxicology studies) were developed to test, with highly predictive potential, the risk of reactogenicity in humans before moving to clinical use. In this review, we provide a historical perspective on how different assays were and can be used to successfully evaluate systemic reactogenicity during clinical development and after licensure.

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Immunogenicity of Vibrio cholerae outer membrane vesicles secreted at various environmental conditions

Cited by 26 publications

References 46 publications

Outer Membrane Vesicle-Host Cell Interactions

Outer Membrane Vesicle-Host Cell Interactions

Ambient pH Alters the Protein Content of Outer Membrane Vesicles, Driving Host Development in a Beneficial Symbiosis

Outer membrane vesicles: moving within the intricate labyrinth of assays that can predict risks of reactogenicity in humans

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