Immunogenicity of Rabies Virus Inactivated by β-Propiolactone, Acetylethyleneimine, and Ionizing Irradiation

Wiktor, T. J.; Aaslestad, H. G.; Kaplan, M. M.

doi:10.1128/aem.23.5.914-918.1972

Cited by 18 publications

(11 citation statements)

References 7 publications

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“…Neither study identified the minimum length of time required for complete inactivation of IHNV infectivity. For comparison, rabies virus treated with 4 to 8 m m BPL or 10 m m BEI is inactivated within 0.5–6 h (Wiktor, Aaslestad & Kaplan 1972; Larghi, Savy, Negel & Rodriguez 1976; Larghi & Nebel 1980). However, this incubation time does not ensure the absence of live residual rabies virus when the innocuity of the vaccine is measured using live animals (Mitchell, Everest & Anderson 1971).…”

Section: Discussionmentioning

confidence: 99%

Inactivated infectious haematopoietic necrosis virus (IHNV) vaccines

Anderson

Clouthier

Shewmaker

et al. 2008

Journal of Fish Diseases

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The inactivation dynamics of infectious haematopoietic necrosis virus (IHNV) by b-propiolactone (BPL), binary ethylenimine (BEI), formaldehyde or heat and the antigenic and immunogenic properties of the inactivated vaccines were evaluated. Chemical treatment of IHNV with 2.7 mm BPL, 1.5 mm BEI or 50 mm formaldehyde abolished virus infectivity within 48 h whereas heat treatment at 50 or 100 degrees C rendered the virus innocuous within 30 min. The inactivated IHNV vaccines were recognized by rainbow trout, Oncorhynchus mykiss, IHNV-specific antibodies and were differentially recognized by antigenic site I or antigenic site II IHNV glycoprotein-specific neutralizing monoclonal antibodies. The BPL inactivated whole virus vaccine was highly efficacious in vaccinated rainbow trout challenged by waterborne exposure to IHNV 7, 28, 42 or 56 days (15 degrees C) after immunization. The formaldehyde inactivated whole virus vaccine was efficacious 7 or 11 days after vaccination of rainbow trout but performed inconsistently when tested at later time points. The other vaccines tested were not efficacious.

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Section: Discussionmentioning

confidence: 99%

Inactivated infectious haematopoietic necrosis virus (IHNV) vaccines

Anderson

Clouthier

Shewmaker

et al. 2008

Journal of Fish Diseases

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“…Numerous reports (9,13,14) on the preparation of experimental vaccines with high levels of immunogenicity have described the inactivation of rabies virus. The effects of various physical and chemical methods of inactivation have been compared by complement fixation, hemagglutination, and infectivity assays.…”

Section: Resultsmentioning

confidence: 99%

Inactivation of rabies virus in reagents used for the fluorescent rabies antibody test

White¹,

Chappell²

1982

J Clin Microbiol

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Procedures for inactivating rabies virus in reagents used for the fluorescent rabies antibody test are described. Mouse brain adsorbing suspensions containing 2109 50% lethal doses of virus per ml were rendered noninfectious by treatment with 0.1% P-propiolactone or by heating at 56°C for .30 min. Viable virus in tissue impression smears was inactivated by acetone fixation at 50°C for .30 min or by immersion in 0.1% ,3-propiolactone at 37°C for 2 h. Inactivated reagents gave specific and sensitive reactions in the fluorescent rabies antibody test. 109 1/ml for CVS and 106.0/ml for street virus.

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“…BPL inactivates viral RNA by alkylation of guanine to form 7- (2-carboxyethyl)guanine (25). However, BPL has also been found to alter viral surface proteins associated with hemagglutination and target cell attachment (31). The rotavirus outer capsid is composed of two major proteins, vp4 and vp7 (3,16).…”

Section: Discussionmentioning

confidence: 99%

Rotavirus-specific protein synthesis is not necessary for recognition of infected cells by virus-specific cytotoxic T lymphocytes

Offit

Greenberg

Dudzik

1989

J Virol

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We found that rotavirus-specific protein synthesis was not necessary for recognition by virus-specific cytotoxic T lymphocytes (CTLs). In addition, CTLs lysed rotavirus-infected target cells prior to production of infectious virus. Target cell processing of rotavirus antigens for presentation to CTLs was enhanced by treatment of rotavirus with trypsin prior to infection; trypsin-induced cleavage of the viral hemagglutinin (vp4) has previously been found to facilitate rotavirus entry into target cells by direct penetration of virions through the plasma membrane. We conclude that sufficient quantities of exogenous viral proteins may be introduced into the cytoplasm for processing by target cells. The mechanism by which rotavirus proteins are processed for presentation to the target cell surface remains to be determined.

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Immunogenicity of Rabies Virus Inactivated by β-Propiolactone, Acetylethyleneimine, and Ionizing Irradiation

Cited by 18 publications

References 7 publications

Inactivated infectious haematopoietic necrosis virus (IHNV) vaccines

Inactivated infectious haematopoietic necrosis virus (IHNV) vaccines

Inactivation of rabies virus in reagents used for the fluorescent rabies antibody test

Rotavirus-specific protein synthesis is not necessary for recognition of infected cells by virus-specific cytotoxic T lymphocytes

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