“…MATERIALS AND METHODS Bacterial strains and growth conditions. P. aeruginosa PAO1 strain H103 of 0 serotype 5 (IATS) (17,26) and a complete set of IATS serotype-specific strains were used for the isolation of LPS and outer membrane and for Formalin fixation of the whole-cell immunogen. The complete set of IATS serotype strains used was type 01 (ATCC 33348), type 02 (ATCC 33349), type 03 (ATCC 33350), type 04 (ATCC 33351), type 05 (ATCC 33352), type 06 (ATCC 33354), type 07 (ATCC 33353), type 08 (ATCC 33355), type 09 (ATCC 33356), type 010 (ATCC 33357), type 011 (ATCC 33358), type 012 (ATCC 33359), type 013 (ATCC 33360), type 014 (ATCC 33361), type 015 (ATCC 33362), type 016 (ATCC 33363), and type 017 (ATCC 33364).…”