Immunogenicity of novel nanoparticle-coated MSP-1 C-terminus malaria DNA vaccine using different routes of administration

Chérif, Mahamoud Sama; Shuaibu, Mohammed Nasir; Kurosaki, Tomoaki; Helegbe, Gideon Kofi; Kikuchi, Mihoko; Yanagi, Tetsuo; Tsuboi, Takafumi; Sasaki, Hidetada; Hirayama, Kenji

doi:10.1016/j.vaccine.2011.09.031

Cited by 40 publications

(28 citation statements)

References 65 publications

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“…Therefore, the DGL/γ-PGA complexes examined in the present study might have accumulated in the spleen via the same mechanism. We previously reported that a pDNA/polyethylenimine/γ-PGA complex, which also induced strong gene expression in the spleen, was an effective DNA vaccine against malaria [25].…”

Section: Discussionmentioning

confidence: 99%

Biodegradable nanoparticles composed of dendrigraft poly-l-lysine for gene delivery

Kodama

Nakamura

Kurosaki

et al. 2014

European Journal of Pharmaceutics and Biopharmaceutics

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We developed novel gene vectors composed of dendrigraft poly-L-lysine (DGL).The transgene expression efficiency of the pDNA/DGL complexes (DGL complexes) was markedly higher than that of the control pDNA/poly-L-lysine complex. However, the DGL complexes caused cytotoxicity and erythrocyte agglutination at high doses.Therefore, γ-polyglutamic acid (γ-PGA), which is a biodegradable anionic polymer, was added to the DGL complexes to decrease their toxicity. The resultant ternary complexes (DGL/γ-PGA complexes) were shown to be stable nanoparticles, and those with γ-PGA to pDNA charge ratios of >8 had anionic surface charges. The transgene expression efficiency of the DGL/γ-PGA complexes was similar to that of the DGL complexes; however, they exhibited lower cytotoxicity and did not induce erythrocyte agglutination at high doses. After being intravenously administered to mice, the DGL6 complex demonstrated high transfection efficiency in the liver, lungs, and spleen, whereas the DGL6/γ-PGA8 complex only displayed high transfection efficiency in the spleen.

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Section: Discussionmentioning

confidence: 99%

Biodegradable nanoparticles composed of dendrigraft poly-l-lysine for gene delivery

Kodama

Nakamura

Kurosaki

et al. 2014

European Journal of Pharmaceutics and Biopharmaceutics

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“…An effective delivery system for malaria vaccination has been described for an NP-coated, MSP-1 DNA-based vaccine which confers protection against lethal Plasmodium yoelii infection in mouse models across various routes of administration. 55 Molecular adjuvants for malaria DNA vaccines based on the modulation of host-cell apoptosis have been described. 56 Field literature describes Vaxfectin (Vical, USA) as having the ability to elevate antibody response and T cell response to each component of a 5-gene Plasmodium falciparum plasmid DNA vaccine mixture.…”

Section: Dna Vaccine and Malariamentioning

confidence: 99%

DNA vaccines: roles against diseases

Khan¹

2013

GERMS

170

121

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Vaccination is the most successful application of immunological principles to human health. Vaccine efficacy needs to be reviewed from time to time and its safety is an overriding consideration. DNA vaccines offer simple yet effective means of inducing broad-based immunity. These vaccines work by allowing the expression of the microbial antigen inside host cells that take up the plasmid. These vaccines function by generating the desired antigen inside the cells, with the advantage that this may facilitate presentation through the major histocompatibility complex. This review article is based on a literature survey and it describes the working and designing strategies of DNA vaccines. Advantages and disadvantages for this type of vaccines have also been explained, together with applications of DNA vaccines. DNA vaccines against cancer, tuberculosis, Edwardsiella tarda, HIV, anthrax, influenza, malaria, dengue, typhoid and other diseases were explored.

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“…9,10,13) We also reported that a pDNA/PEI/γ-polyglutamic acid complex, which also induced strong gene expression (more than 1×10 6 RLU/g tissue) in the spleen, was an effective DNA vaccine against malaria and melanoma. 27,28) pDNA/PEI/DOPS complex also showed gene expression at about 1×10 6 RLU/g tissue in the spleen. The pDNA/PEI/DOPS complex might be effective as DNA vaccine, although further study is necessary.…”

Section: 23)mentioning

confidence: 95%

Splenic Gene Delivery System Using Self-assembling Nano-complex with Phosphatidylserine Analog

et al. 2015

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The recognition of phosphatidylserine on the erythrocyte membrane mediates erythrophagocytosis by resident spleen macrophages. The application of phosphatidylserine to a gene vector may be a novel approach for splenic drug delivery. Therefore, we chose 1,2-dioleoyl-sn-glycero-3-phospho-L-serin (DOPS) as an analogue of phosphatidylserine for splenic gene delivery of plasmid DNA (pDNA). In the present study, we successfully prepared a stable pDNA ternary complex using DOPS and polyethyleneimine (PEI) and evaluated its efficacy and safety. The pDNA/PEI complex had a positive charge and showed high transgene efficacy, although it caused cytotoxicity and agglutination. The addition of DOPS changed the ζ-potential of the pDNA/PEI complex to negative. It is known that anionic complexes are not taken up well by cells. Surprisingly, however, the pDNA/PEI/DOPS complex showed relatively high transgene efficacy in vitro. Fluorescence microscope observation revealed that the pDNA/PEI/DOPS complex internalized the cells while maintaining the complex formation. The injection of the pDNA/PEI complex killed most mice within 24 h at high doses, although all mice in the pDNA/PEI/DOPS complex group survived. The ternary complex with DOPS showed markedly better safety compared with the pDNA/PEI complex. The pDNA/PEI/DOPS complex showed high gene expression selectively in the spleen after intravenous injection into mice. Thus the ternary complex with DOPS can be used to deliver pDNA to the spleen, in which immune cells are abundant. It appears to have an excellent safety level, although further study to determine the mechanism of action is necessary.Key words gene delivery; spleen; 1,2-dioleoyl-sn-glycero-3-phospo-L-serin; plasmid DNA; ternary complexIn gene delivery, non-viral vectors, including cationic polymers, have several advantages, e.g., they are non-immunogenic; cause few acute toxicities; and their structural and chemical properties can be tightly controlled, which allows vehicles that are suitable for mass production to be designed.1,2) Among non-viral gene vectors, polyethylenimine (PEI) is a popular cationic polymer and show high gene expression in in vitro and in vivo, because of specific mechanisms such as binding to the cell surface, being taken up by the endocytotic pathway, and release of plasmid DNA (pDNA) from endosomes via the so-called "proton sponge mechanism." On the other hand, PEI caused nonspecific gene expressions, high cytotoxicity, and aggregation with blood components because of their strong cationic charge. Recharging cationic complex with anionic compound was reported to be a promising method for overcoming these toxicities.Phosphatidylserine is a biocompatible phospholipid that has a negative charge at biological pH and is a component of the cellular membrane. In normal cells, phosphatidylserines are restricted in the inner leaflet of the cellular membrane by the function of ATP-dependent transporters. [3][4][5] In contrast, in apoptotic cells or aging erythrocytes, phosphatidylserines exposed on the...

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Immunogenicity of novel nanoparticle-coated MSP-1 C-terminus malaria DNA vaccine using different routes of administration

Abstract: (Vaccine 29 (2011)

Cited by 40 publications

References 65 publications

Biodegradable nanoparticles composed of dendrigraft poly-l-lysine for gene delivery

Biodegradable nanoparticles composed of dendrigraft poly-l-lysine for gene delivery

DNA vaccines: roles against diseases

Splenic Gene Delivery System Using Self-assembling Nano-complex with Phosphatidylserine Analog

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