Immunogenicity of meningococcal PorA formulations encapsulated in biodegradable microspheres

Arigita, Carmen; Berg, Joost van den; Wensink, Karin; Steenbergen, Mies van; Hennink, Wim E.; Crommelin, Daan J.A.; Kersten, Gideon; Jiskoot, Wim

doi:10.1016/j.ejps.2003.09.006

Cited by 8 publications

(4 citation statements)

References 32 publications

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“…According to the literature and similarly to other reported methacrylates, VMA mainly couples to the 6 C of the mannose residues of the mannan side chains [18,25]. Then by the Michael addition mechanism, the thiol acting as a nucleophile reacts with grafted methacrylate (Scheme 1).…”

Section: Synthesis Of Amphiphilic Mannan-c 16mentioning

confidence: 69%

Supramolecular assembled nanogel made of mannan

Ferreira

Pereira

Sampaio

et al. 2011

Journal of Colloid and Interface Science

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The supramolecular assembly of amphiphilic mannan, synthesized by the Michael addition of hydrophobic 1-hexadecanethiol to vinyl methacrylated mannan, originates in aqueous medium the formation of a nanogel, stabilized by hydrophobic interactions among alkyl chains. The critical aggregation concentration, calculated by fluorescence spectroscopy ranged between 0.002 and 0.01 mg/mL, depending on the polymer degree of substitution. The cryo-field emission scanning electron microscopy showed spherical macromolecular micelles with diameters between 100 and 500 nm. The dynamic light scattering analysis revealed a polydisperse colloidal system, with mean hydrodynamic diameter between 50 and 140 nm, depending on the polymer degree of substitution. The nanogel is negatively charged, stable over a 6 months storage period, and stable at pH 3-8, salt or urea solutions. Bovine serum albumin and curcumin were spontaneously incorporated in the nanogel, being stabilized by the hydrophobic domains, opening the possibility for future applications as potential delivery systems for therapeutic molecules. In vitro assays were carried out to characterize the biocompatibility of the nanogel. A toxic effect of mannan-C(16) was observed, specific to mouse macrophage-like cell line J774, not affecting mouse embryo fibroblast cell line 3T3 viability.

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Section: Synthesis Of Amphiphilic Mannan-c 16mentioning

confidence: 69%

Supramolecular assembled nanogel made of mannan

Ferreira

Pereira

Sampaio

et al. 2011

Journal of Colloid and Interface Science

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“…In the first step, methacrylated mannan was obtained by coupling HEMA-CI to mannan. A majority of HEMA groups, according to several authors, are likely coupled to the 6 C of the mannose residues of the grafts. , In the second step, the thiol acting as a nucleophile reacted with grafted methacrylate by a Michael addition mechanism, as shown in Scheme .…”

Section: Resultsmentioning

confidence: 99%

Self-Assembled Nanogel Made of Mannan: Synthesis and Characterization

2010

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Amphiphilic mannan (mannan-C(16)) was synthesized by the Michael addition of hydrophobic 1-hexadecanethiol (C(16)) to hydroxyethyl methacrylated mannan (mannan-HEMA). Mannan-C(16) formed nanosized aggregates in water by self-assembly via the hydrophobic interaction among C(16) molecules as confirmed by hydrogen nuclear magnetic resonance ((1)H NMR), fluorescence spectroscopy, cryo-field emission scanning electron microscopy (cryo-FESEM), and dynamic light scattering (DLS). The mannan-C(16) critical aggregation concentration (cac), calculated by fluorescence spectroscopy with Nile red and pyrene, ranged between 0.04 and 0.02 mg/mL depending on the polymer degree of substitution of C(16) relative to methacrylated groups. Cryo-FESEM micrographs revealed that mannan-C(16) formed irregular spherical macromolecular micelles, in this work designated as nanogels, with diameters ranging between 100 and 500 nm. The influence of the polymer degree of substitution, DS(HEMA) and DS(C(16)), on the nanogel size and zeta potential was studied by DLS at different pH values and ionic strength and as a function of mannan-C(16) and urea concentrations. Under all tested conditions, the nanogel was negatively charged with a zeta potential close to zero. Mannan-C(16) with higher DS(HEMA) and DS(C(16)) values formed larger nanogels and were also less stable over a 6 month storage period and at concentrations close to the cac. When exposed to solutions of different pH and aggressive conditions of ionic strength and urea concentration, the size of mannan-C(16) varied to some extent but was always in the nanoscale range.

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“…Controlled release devices have been used to encapsulate a large number of antigens against diseases as diverse as the plague [88,89], bacterial pneumonia [90], meningitis [91], tuberculosis [92], brucellosis [93], and anthrax [70]. On the whole, these studies demonstrate a similar adjuvant effect of controlled release devices that results in a stronger immune response, but with additional stability issues.…”

Section: Other Antigensmentioning

confidence: 96%