Immunogenicity of Inactivated Newcastle Disease Virus Preparations

Ms, Hofstad; Jc, Picken; Ke, Collins; Hw, Yoder

doi:10.2307/1587880

Cited by 8 publications

(3 citation statements)

References 4 publications

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“…The inactivation of NDV was done by 0.1% formalin at 37°C/ 18 h, and two successive passages of the inactivated vaccine in the SPF egg were performed to confirm that vaccine is free from live virus according to [30]. The inactivated fluids were used for preparing WO vaccine according to [31] with a ratio of the aqueous phase to oil phase at 30:70 and WOW vaccine according to [20].…”

Section: Preparation Of the Inactivated Vaccines From Ndv/168 Strainmentioning

confidence: 99%

Molecular and biological characterization of the immunological potency of Newcastle disease virus oil emulsion–inactivated vaccines prepared from field isolate obtained from vaccinated chickens outbreak

Hassan

El-Azeem

Selim³

et al. 2019

Braz J Microbiol

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This study was conducted to characterize the immunological parameters of chickens vaccinated with two formulated inactivated vaccines, water in oil (WO) and water in oil in water (WOW), prepared from velogenic Newcastle disease virus (vNDV) genotype VIIj isolated from outbreak among vaccinated chickens. Six groups (G1-G6) of commercial broiler chickens were established (n = 20). The G1-G3 were received homologous (WO and WOW) and heterologous (LaSota) inactivated vaccines, respectively. The G4 was vaccinated with live heterologous (LaSota) vaccine, while G5 and G6 were kept as control positive and control negative non-vaccinated groups. The antibody titers were measured against vNDV and LaSota antigens using hemagglutination inhibition (HI) test, the cytokine gene expressions of IFNγ, IL1β, IL4, IL6, IL8, and IL18 were quantified using realtime RT-PCR, and the virus shedding was titrated on chicken embryo fibroblast cells after challenging by vNDV. The classical clinical signs and 100% mortality were observed only in G5 after vNDV challenging. The highest HI titers were detected in G1, G2, and G3 using NDV/168 antigen with no significant differences among them. These groups showed higher HI titer than G4 (2-4log2). Cytokine gene expression of IFNγ, IL1, IL6, IL8, and IL18 were significantly downregulated in vaccinated chickens with upregulation of IL4 than non-vaccinated challenge group. Viral shedding titers were significantly (0.0001, p ≤ 0.001) reduced in all samples form vaccinated chickens. In conclusion, the prepared vaccines produced highly efficient immunological responses and could be used for controlling the NDV infection.

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Section: Preparation Of the Inactivated Vaccines From Ndv/168 Strainmentioning

confidence: 99%

Molecular and biological characterization of the immunological potency of Newcastle disease virus oil emulsion–inactivated vaccines prepared from field isolate obtained from vaccinated chickens outbreak

Hassan

El-Azeem

Selim³

et al. 2019

Braz J Microbiol

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“…La Sota strain: It was done according to Hofstad et al, (1963) using formalin (37%) Analar, BDH that was added to the harvested allantoic fluid in a final concentration of 0.1%, left for 18 hours at room. Safety of the inactivated virus was tested by injecting 0.2 ml of the inactivated fluid into 9-11 days ECE through allantoic sac for 3 blind passages.…”

Section: Inactivation Of the Propagated Ndvmentioning

confidence: 99%

A trial to improve stability and immunogenicity of inactivated NDV vaccine with paraffin oil adjuvant using aluminum stearate

El-Bagoury

El-Habbaa

M.H.M.³

et al. 2015

Benha Veterinary Medical Journal

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A B S T R A C TInactivated Newcastle disease virus (NDV) vaccines were prepared using paraffin oil, paraffin oil and aluminum stearate (Al.St.) and Montanide oil ISA 71VG as adjuvants. All experiments gave the priority to the inactivated NDV vaccines with paraffin oil adjuvant and Al.St. 4-5 mg/dose including stability (drop test, centrifugation and real time test) and viscosity (dynamic and relative) over NDV vaccine adjuvant with paraffin oil alone that showed reduced stability, however NDV vaccine with paraffin oil and Al.St. 6mg/dose and 7mg/dose showed high values of viscosity indicating hard injectability. Chicks vaccinated with inactivated NDV vaccines with paraffin oil adjuvant and Al.St. (4, 5, 6 and 7 mg/dose) gave higher cell mediated immune response (lymphocyte blastogenesis and macrophage activity), humoral immune responses (using haemagglutination inhibition test) and 100% protection upon challenge experiment in comparison to that vaccinated with NDV vaccine with paraffin oil adjuvant alone that showed 93.3% and 86.66% protection for vaccines used immediately after preparation and the others used after one year of storage at 4°C, respectively. Chicks vaccinated with NDV vaccines with paraffin oil adjuvant and Al.St showed slightly lower values of both cellular and humoral immune responses than that of NDV vaccine adjuvant with Montanide ™ ISA 71VG. In conclusion, inactivated NDV vaccine prepared with paraffin oil adjuvant and aluminum stearate (4 mg/dose and 5 mg/dose) were superior for that adjuvant on paraffin oil alone, as they improved stability and potency of the vaccine, and were with the same quality to that of NDV vaccine adjuvant on the imported Montanide ISA 71VG.

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“…recommended that more efficient immunizing strains be used, such as LaSota. Since the immunogenicity of a strain is directly related to its virulence (66), the more efficient immunizing strains are also those which induce the most severe respiratory reactions (6).…”

mentioning

confidence: 99%

Pathology of trachea in turkeys exposed by aerosol to Newcastle disease virus

Abdul-Aziz

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Immunogenicity of Inactivated Newcastle Disease Virus Preparations

Cited by 8 publications

References 4 publications

Molecular and biological characterization of the immunological potency of Newcastle disease virus oil emulsion–inactivated vaccines prepared from field isolate obtained from vaccinated chickens outbreak

Molecular and biological characterization of the immunological potency of Newcastle disease virus oil emulsion–inactivated vaccines prepared from field isolate obtained from vaccinated chickens outbreak

A trial to improve stability and immunogenicity of inactivated NDV vaccine with paraffin oil adjuvant using aluminum stearate

Pathology of trachea in turkeys exposed by aerosol to Newcastle disease virus

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