Immunogenicity of extracellular products from an inactivated vaccine against Aeromonas veronii TH0426 in koi, Cyprinus carpio

Song, Ming-Fang; Kang, Yuan-Huan; Zhang, Dong-Xing; Chen, Long; Bi, Jian-Fei; Zhang, Haipeng; Zhang, Lei; Ai-dong, Qian

doi:10.1016/j.fsi.2018.07.004

Cited by 39 publications

(14 citation statements)

References 38 publications

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“…A. veronii is a serious pathogen that causes high mortality rates and economic losses in the aquaculture industry [18]. Although antimicrobials can treat bacterial disease, their widespread and excessive use exacerbates the development of drugs that are resistant to bacteria [19].…”

Section: Discussionmentioning

confidence: 99%

Oral Administration of Lactobacillus Casei Expressing Flagellin A Protein Confers Effective Protection against Aeromonas Veronii in Common Carp, Cyprinus Carpio

Tian

Kang

Chu

et al. 2019

IJMS

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Aeromonas veronii is a pathogen capable of infecting humans, livestock and aquatic animals, resulting in serious economic losses. In this study, two recombinant Lactobacillus casei expressing flagellin A (FlaA) of A. veronii, Lc-pPG-1-FlaA (surface-displayed) and Lc-pPG-2-FlaA (secretory) were constructed. The immune responses in fish administered with recombinant L. casei were evaluated. The two recombinant L. casei were orally administered to common carp, which stimulated high serum IgM and induced higher ACP, AKP, SOD and LYZ activity. Using qRT-PCR, the expression of IL-10, IL-8, IL-1β, TNF-α and IFN-γ in the tissue of fish immunized with recombinant L. casei was significantly (p < 0.05) upregulated, which indicated that recombinant L. casei could activate the innate immune system to trigger the cell immune response and inflammatory response. Furthermore, recombinant L. casei was able to survive the intestinal environment and colonize in intestine mucosal. The study showed that after being challenged by A. veronii, fish administered with Lc-pPG-1-FlaA (70%) and Lc-pPG-2-FlaA (50%) had higher survival rates compared to Lc-pPG and PBS, indicating that recombinant L. casei might prevent A. veronii infection by activating the immune system to trigger immune responses. We demonstrated that flagellin as an antigen of vaccine, is acceptable for preventing A. veronii infection in fish. The recombinant L. casei expressing FlaA may be a novel mucosal vaccine for treating and controlling A. veronii.

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Section: Discussionmentioning

confidence: 99%

Oral Administration of Lactobacillus Casei Expressing Flagellin A Protein Confers Effective Protection against Aeromonas Veronii in Common Carp, Cyprinus Carpio

Tian

Kang

Chu

et al. 2019

IJMS

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“…Among fish-associated bacteria, A. veronii is found in fresh water in association with vertebrates and invertebrates with virulence factors such as enterotoxin, flagella and outer membrane proteins that affect fishes and other aquatic animals with high mortality rate and economic losses [41][42][43][44][45][46][47][48]. Pseudomonas entomophila is commonly found in insects and soil and is closely related to P. putida [49].…”

Section: Lrz9mentioning

confidence: 99%

Probiotic Bacteria with High Alpha-Gal Content Protect Zebrafish against Mycobacteriosis

et al. 2021

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Mycobacteriosis affects wild fish and aquaculture worldwide, and alternatives to antibiotics are needed for an effective and environmentally sound control of infectious diseases. Probiotics have shown beneficial effects on fish growth, nutrient metabolism, immune responses, disease prevention and control, and gut microbiota with higher water quality. However, the identification and characterization of the molecules and mechanisms associated with probiotics is a challenge that requires investigation. To address this challenge, herein we used the zebrafish model for the study of the efficacy and mechanisms of probiotic interventions against tuberculosis. First, bacteria from fish gut microbiota were identified with high content of the surface glycotope Galα1-3Galβ1-(3)4GlcNAc-R (α-Gal) that has been shown to induce protective immune responses. The results showed that probiotics of selected bacteria with high α-Gal content, namely Aeromonas veronii and Pseudomonas entomophila, were biosafe and effective for the control of Mycobacterium marinum. Protective mechanisms regulating immunity and metabolism activated in response to α-Gal and probiotics with high α-Gal content included modification of gut microbiota composition, B-cell maturation, anti-α-Gal antibodies-mediated control of mycobacteria, induced innate immune responses, beneficial effects on nutrient metabolism and reduced oxidative stress. These results support the potential of probiotics with high α-Gal content for the control of fish mycobacteriosis and suggested the possibility of exploring the development of combined probiotic treatments alone and in combination with α-Gal for the control of infectious diseases.

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“…veronii infection in fish has already been reported to cause mortality and thereby economic loss to the aquaculture (Song et al, 2018). A. veronii isolated from the diseased fish has previously been reported to cause fish sepsis and ulcerative disease syndrome (Hassan et al, 2017).…”

Section: F I G U R E 2 Percentage Of Mortality Inmentioning

confidence: 99%

Disease inductive potential of Aeromonas veronii XhG1.2 isolated from Xiphophorus hellerii in selected ornamental fish

Das

Aswani

Jose

et al. 2021

Aquaculture Research

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Aeromonas spp. are opportunistic pathogens which cause diseases in ornamental fish.But the degree of infection and the severity of disease caused by various Aeromonas spp. are generally being reported to be strain specific. Therefore, the present study focused on the disease induction ability of previously isolated Aeromonas veronii XhG1.2 in Xiphophorus hellerii in comparison with A. hydrophila (MTCC 1739). Further, the ability of A. veronii XhG1.2 to cause disease in other selected ornamental fish such as Poecilia sphenops (molly), Carassius auratus (goldfish) and Cyprinus carpio (common carp) has also been studied. Here, the intramuscular injection of A. veronii XhG1.2 was observed to cause disease incidence and increased mortality in all the selected ornamental fish. The mortality observed was 100, 100 and 88.3%, respectively, for P. sphenops, C. auratus and C. carpio within the 48-h period. Histopathological analysis of gill, liver and intestine of all the XhG1.2 treated fish further confirmed its role in fish disease. The results of the study are indicative of emergence of Aeromonas spp. with the ability to cause disease in wide range of ornamental fish.

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Immunogenicity of extracellular products from an inactivated vaccine against Aeromonas veronii TH0426 in koi, Cyprinus carpio

Cited by 39 publications

References 38 publications

Oral Administration of Lactobacillus Casei Expressing Flagellin A Protein Confers Effective Protection against Aeromonas Veronii in Common Carp, Cyprinus Carpio

Oral Administration of Lactobacillus Casei Expressing Flagellin A Protein Confers Effective Protection against Aeromonas Veronii in Common Carp, Cyprinus Carpio

Probiotic Bacteria with High Alpha-Gal Content Protect Zebrafish against Mycobacteriosis

Disease inductive potential of Aeromonas veronii XhG1.2 isolated from Xiphophorus hellerii in selected ornamental fish

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