Immunogenicity of a bivalent protein as a vaccine against Edwardsiella anguillarum and Vibrio vulnificus in Japanese eel (Anguilla japonica)

Guo, Songlin; Hu, Linlin; Feng, Jia; Lin, Peng; He, Le; Yan, Qingpi

doi:10.1002/mbo3.766

Cited by 19 publications

(10 citation statements)

References 42 publications

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“…At present, it is believed that the pathogenesis of V. vulnificus infection mainly depends on the synergistic effect of multiple virulence factor genes. In addition to the well-defined main virulence genes vvhA and rtxA , other virulence genes, such as Wza (capsular polysaccharide outer membrane protein) and OmpU (conserved outer membrane porin), are thought to play a role in the pathogenic process of V. vulnificus [ 28 ]. Wza plays an important role in protecting bacteria from the host’s innate immune response and is widespread among pathogenic Vibrio spp.…”

Section: Methodsmentioning

confidence: 99%

Identification and whole-genome sequencing analysis of Vibrio vulnificus strains causing pearl gentian grouper disease in China

Gao

et al. 2022

BMC Microbiol

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Vibrio vulnificus is a pathogenic bacterium that causes disease in marine fish, affecting fish farming and human health worldwide. In May 2021, in the Bohai Bay region, a disease broke out in commercially farmed pearl gentian grouper (♀Epinephelus fuscoguttatus × ♂Epinephelus lanceolatus), causing huge economic losses. The diseased fish had skin lesions, water accumulation in their abdomens, and showed tissue and organ damage. V. vulnificus biotype 2 has been reported in eels and other marine fish, but it is less reported in pearl gentian grouper. In this study, the pathogenic strain isolated from diseased fish was identified as V. vulnificus EPL 0201 biotype 2 on the basis of physiological and biochemical characteristics and the results of 16S rRNA gene and gyrB sequencing, virulence gene detection, and recursive infection experiments. To gain a comprehensive understanding of the pathogenicity and drug resistance of this strain, whole-genome sequencing was performed. Whole-genome analysis showed that the gene map of this strain was complete. The Virulence Factor Database annotation results showed that this strain had the key virulence factor genes vvhA and rtxA, which cause host disease. In addition, this strain had genes conferring resistance against cephalosporins, aminoglycosides, tetracyclines, and sulfonamides. Antimicrobial susceptibility testing confirmed the presence of these resistance genes identified in the genome. The results of this study show that V. vulnificus EPL 0201 biotype 2 is a multi-drug resistant strain with high pathogenicity.

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Section: Methodsmentioning

confidence: 99%

Identification and whole-genome sequencing analysis of Vibrio vulnificus strains causing pearl gentian grouper disease in China

Gao

et al. 2022

BMC Microbiol

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“…Other outer membrane proteins (Omp)‐based vaccines have been developed, the most used being OmpU, OmpA and OmpII from V. vulnificus , E. anguillarum and A. hydrophila , respectively (Table 5). After vaccination and challenge with a given pathogen, eels vaccinated with Omp generally had significantly higher relative survival than those vaccinated with control solution (Guo et al., 2019, 2020; He et al., 2020; Le et al., 2018; LiHua et al., 2019).…”

Section: Potential Treatmentmentioning

confidence: 99%

“…However, the treatment did not appear to be effective for A. anguilla (Knopf & Lucius, 2008). While most vaccines are developed to protect against a single pathogen, promising bivalent (Guan et al., 2011; Guo et al., 2019) and trivalent (Zhao et al., 2020) vaccines are also being developed and could protect against different pathogens.…”

Section: Potential Treatmentmentioning

confidence: 99%

Anguilla sp. diseases diagnoses and treatments: The ideal methods at the crossroads of conservation and aquaculture purposes

Parchemin

Tapissier‐Bontemps

Sasal

et al. 2022

Journal of Fish Diseases

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Anguilla anguilla, A. japonica and A. rostrata are the most fished and consumed eel species. However, these species are Critically Endangered, Endangered and Endangered, respectively. A combination of factors is thought to be responsible for their decline including fisheries, climate change, habitat destruction, barriers to migration, pollution and pathogens. Among them, viruses, bacteria and parasites are causing weakening of wild eels and serious economic losses for fishermen and eel farmers. Early detection of pathogens is essential to provide appropriate responses both for conservation reasons and to limit economic losses. Classic diagnosis approaches are time consuming and invasive and usual treatments, for example, antipathogenic substances are becoming obsolete because of pathogen resistance and environmental impact problems. The need for early and non‐invasive diagnostic methods as well as effective and environmentally friendly treatments has increased. Vaccine development and diet supplementation have known a growing interest since their use could allow prevention of diseases. In this review, we summarize the main pathogens—viruses, bacteria and parasites—of the three northern temperate eel species, the methods used to detect these pathogens and the different treatments used. We discussed and highlighted the need for non‐invasive, rapid and efficient detection methods, as well as effective and environmentally friendly treatments for both conservation and aquaculture purposes.

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“…Due to the good immunogenicity and immune protective effect, outer membrane proteins (Omps) on the surface of Gram-negative pathogenic bacteria have gradually become a research hot spot in the development of fish vaccines (Guo, Feng, Lin, Cao, & Zhao, 2015a;Guo et al, 2019;Guo, Lu, et al, 2015;Guo, Wang, Guan, Feng, & Lu, 2013;. It was reported that a single Omp from V. vulnificus, E. anguillarum and A. hydrophila had protective effects in mice, Labeo rohita, Japanese eel and American eel (Duan et al, 2019;He et al, 2018;Yadav, Sahoo, & Dixit, 2014).…”

Section: Introductionmentioning

confidence: 99%

“…It was reported that a single Omp from V. vulnificus, E. anguillarum and A. hydrophila had protective effects in mice, Labeo rohita, Japanese eel and American eel (Duan et al, 2019;He et al, 2018;Yadav, Sahoo, & Dixit, 2014). In recent years, researchers have used the fusion PCR technology to construct a double expression vector of Omp genes and studied the immunogenicity of bivalent expressed Omp, and the results of their studies indicated good immunogenicity and effective protection after the immunization with different bivalent Omps in eels (Guo, Feng, et al, 2015;Guo et al, 2013Guo et al, , 2019Guo, Lu, et al, 2015). However, these bivalent Omps could not effectively protect against infection by these three pathogens simultaneously.…”

Section: Introductionmentioning

confidence: 99%

Construction expression and immunogenicity of a novel trivalent outer membrane protein (OmpU‐A‐II) from three bacterial pathogens in Japanese eels (Anguilla japonica)

Zhao

Zhai

et al. 2020

Journal of Fish Diseases

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Vibrio vulnificus, Edwardsiella anguillarum and Aeromonas hydrophila are three common bacterial pathogens in cultivated eels. To protect farming eels from infection by these pathogens, a trivalent outer membrane protein (OMP) containing partial sequences of OmpU from V. vulnificus, OmpA from E. anguillarum and OmpII from A. hydrophila was expressed and purified; then, the OMP was used as a vaccine to immunize Japanese eels (Anguilla japonica). Whole-blood cell proliferation, antibody titres and complement and lysozyme activities were detected at different days postimmunization (dpi), and the relative per cent survival (RPS) was determined after eels were infected with V. vulnificus, E. anguillarum or A. hydrophila at 28 dpi. The results showed that the OMP significantly stimulates the antibody titres. At 14 days after the challenge (i.e. at 28 dpi), the RPS of OMP against V. vulnificus, E. anguillarum and A. hydrophila was 20%, 70% and 11.1%, respectively. The construction, expression and immunogenicity of a trivalent Omp were reported for the first time, and this study will provide a valuable reference for the development of fish multiplex vaccines. K E Y W O R D SAeromonas hydrophila, Edwardsiella anguillarum, immunogenicity, trivalent Omp, Vibrio vulnificus How to cite this article: Zhao J, Wu L, Zhai S, Lin P, Guo S.Construction, expression and immunogenicity of a novel trivalent outer membrane protein (OmpU-A-II) from three bacterial pathogens in Japanese eels (Anguilla japonica). J Fish

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Immunogenicity of a bivalent protein as a vaccine against Edwardsiella anguillarum and Vibrio vulnificus in Japanese eel (Anguilla japonica)

Cited by 19 publications

References 42 publications

Identification and whole-genome sequencing analysis of Vibrio vulnificus strains causing pearl gentian grouper disease in China

Identification and whole-genome sequencing analysis of Vibrio vulnificus strains causing pearl gentian grouper disease in China

Anguilla sp. diseases diagnoses and treatments: The ideal methods at the crossroads of conservation and aquaculture purposes

Construction expression and immunogenicity of a novel trivalent outer membrane protein (OmpU‐A‐II) from three bacterial pathogens in Japanese eels (Anguilla japonica)

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