Immunogenicity and protective efficacy of mucosal delivery of recombinant hcp of Campylobacter jejuni Type VI secretion system (T6SS) in chickens

Singh, Ankita; Nisaa, Khairun; Bhattacharyya, S.; Mallick, Amirul Islam

doi:10.1016/j.molimm.2019.04.016

Cited by 30 publications

(25 citation statements)

References 96 publications

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“…C. jejuni (18aM) was isolated from the cecal content of commercial broiler chickens and processed as per the procedure described elsewhere [54]. Briefly, samples were serially diluted in autoclaved distilled water and 0.1 mL from 10 −3 dilution was plated onto Blood Free Campylobacter Selectivity Agar Base media (HiMedia, India) having CAT Selective Supplement (cefoperazone 8 mg/L, amphotericin 10 mg/L, and teicoplanin 4 mg/L) (HiMedia) followed by incubation for 48 h. Colonies found positive for C. jejuni were next grown in Mueller Hinton broth (HiMedia) supplemented with CAT supplement for 48 h under microaerophilic condition (10 % CO 2 , 5 % O 2, and 85 % N 2 ) at 42 °C using microaerophilic generating gas pack (Anaerogas pack 3.5 L, HiMedia)…”

Section: Methodsmentioning

confidence: 99%

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Naturally secreted bacterial outer membrane vesicles: potential platform for a vaccine againstCampylobacter jejuni

Singh

Khan

Ghosh

et al. 2020

Preprint

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Acute diarrheal illness and gastroenteritis caused by Campylobacter jejuni ( C. jejuni ) infection remain significant public health risks in developing countries with substantial mortality and morbidity in humans, particularly in children under the age of five. Despite improved global awareness in sanitation and hygiene practices, including food safety measures, C. jejuni infections continue to rise even across the developed nations and no vaccine is currently available for humans. Genetic diversities among C. jejuni strains as well as limited understanding of immunological correlates of host protection remain primary impediments for developing an effective vaccine against C. jejuni . Given the role of bacterial outer membrane-associated proteins in intestinal adherence and invasion as well as modulating dynamic interplay between host and pathogens, bacterial outer membrane vesicles (OMVs) have emerged as potential vaccine platforms against a number of enteric pathogens, including C. jejuni . In the present study, we describe a mucosal vaccine strategy using chitosan (CS) coated OMVs (CS-OMVs) to induce specific immune responses against C. jejuni in mice. However, considering the challenges of mucosal delivery of OMVs in terms of exposure to variable pH, risk of enzymatic degradation, rapid gut transit, and low permeability across the intestinal epithelium, we preferentially used CS as a non-toxic, mucoadhesive polymer to coat OMVs. Mucosal administration of CS-OMVs induced high titre of systemic (IgG) and local (secretory IgA) antibodies in mice. The neutralizing ability of secretory IgA (sIgA) produced in the intestine was confirmed by in vitro inhibition of cell adherence and invasion of C. jejuni while in vivo challenge study in OMVs immunized mice showed a significant reduction in cecal colonization of C. jejuni . Moreover, to investigate the immunological correlates of the observed protection, present data suggest OMVs driven T cell proliferation with an increased population of CD4 + and CD8 + T cells. In addition to antibody isotype profile, significant upregulation of IFN-γ and IL-6 gene expression in mesenteric lymph nodes collected from OMVs immunized mice further suggests that mucosal delivery of OMVs promotes a Th1/Th2 mixed type immune responses. Together, we provide strong experimental evidence that as an acellular and non-replicating canonical end product of bacterial secretion, mucosal delivery of OMVs may represent a promising platform for developing an effective vaccine against C. jejuni .

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Section: Methodsmentioning

confidence: 99%

“…For mucosal delivery, chitosan was cross-linked with sodium tripolyphosphate (CS-TPP) following the protocol described previously in our lab with some modifications [54]. Briefly, 700 μL of freshly isolated OMVs (~70μg) was mixed with 3.3 mL of CS solution (0.05 % w/v) followed by drop-wise addition of 1 mL of TPP solution (0.1% w/v).…”

Section: Methodsmentioning

confidence: 99%

Naturally secreted bacterial outer membrane vesicles: potential platform for a vaccine againstCampylobacter jejuni

Singh

Khan

Ghosh

et al. 2020

Preprint

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“…Campylobacter jejuni is a major source of food poisoning in the poultry industry. The hemolysin co-regulated protein (hcp) is believed to play a vital role related to the virulence of this bacteria as a component of the bacterial type VI secretion system, which is a phage like puncturing device [ 105 ].…”

Section: Biomedical Applications Of Nanochitosanmentioning

confidence: 99%

“…A potential vaccine candidate against C. jejuni colonization in chicken was developed by entrapping recombinant hcp in chitosan-sodium tripolyphosphate nanoparticles, and the mucosal administration was found to have a significant effect [ 105 ]. Glucose oxidase (GOx) immobilized on CSNPs via covalent attachment (CA), enzyme coating (EC), enzyme precipitation coating (EPC), and magnetic nanoparticle-incorporated EPC methods were tested for the inhibition of S. aureus by enzymatically produced H 2 O 2 in the presence of glucose.…”

Section: Biomedical Applications Of Nanochitosanmentioning

confidence: 99%

Agricultural and Biomedical Applications of Chitosan-Based Nanomaterials

Bandara

Carson

et al. 2020

Nanomaterials

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Chitosan has emerged as a biodegradable, nontoxic polymer with multiple beneficial applications in the agricultural and biomedical sectors. As nanotechnology has evolved as a promising field, researchers have incorporated chitosan-based nanomaterials in a variety of products to enhance their efficacy and biocompatibility. Moreover, due to its inherent antimicrobial and chelating properties, and the availability of modifiable functional groups, chitosan nanoparticles were also directly used in a variety of applications. In this review, the use of chitosan-based nanomaterials in agricultural and biomedical fields related to the management of abiotic stress in plants, water availability for crops, controlling foodborne pathogens, and cancer photothermal therapy is discussed, with some insights into the possible mechanisms of action. Additionally, the toxicity arising from the accumulation of these nanomaterials in biological systems and future research avenues that had gained limited attention from the scientific community are discussed here. Overall, chitosan-based nanomaterials show promising characteristics for sustainable agricultural practices and effective healthcare in an eco-friendly manner.

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“…In the United States, each year around 2.4 million Campylobacter related human illness cases are reported (Sean et al, 1999). Recently, CS NPs was used to deliver recombinant hemolysin co-regulated protein of C. jejuni in chickens (Singh et al, 2019), and used molecular techniques to confirm the loaded antigen and found it was intact in the CS NPs, and oral vaccination increased the pre-challenge secretory IgA and systemic IgY antibodies. Vaccination also increased NFkB, IL-1β, IL-8, IL-6, IFN-γ, and IL-17A gene expression, and reduced the challenge C. jejuni load in cecum.…”

Section: Cs Nps Based Vaccines For Bacterial Infectious Diseasementioning

confidence: 99%

Chitosan Nanoparticle Based Mucosal Vaccines Delivered Against Infectious Diseases of Poultry and Pigs

Renu

Renukaradhya

2020

Front. Bioeng. Biotechnol.

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Immunogenicity and protective efficacy of mucosal delivery of recombinant hcp of Campylobacter jejuni Type VI secretion system (T6SS) in chickens

Cited by 30 publications

References 96 publications

Naturally secreted bacterial outer membrane vesicles: potential platform for a vaccine againstCampylobacter jejuni

Naturally secreted bacterial outer membrane vesicles: potential platform for a vaccine againstCampylobacter jejuni

Agricultural and Biomedical Applications of Chitosan-Based Nanomaterials

Chitosan Nanoparticle Based Mucosal Vaccines Delivered Against Infectious Diseases of Poultry and Pigs

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