Immunogenicity and protection-inducing ability of recombinant Plasmodium vivax rhoptry-associated protein 2 in Aotus monkeys: A potential vaccine candidate

Rojas-Caraballo, José; Mongui, Alvaro; Giraldo, Manuel A.; Delgado, Gabriela; Granados, Diana; Millan-Cortes, Diana; Martinez, Paola; Rodríguez, Ricaurte; Patarroyo, Manuel A.

doi:10.1016/j.vaccine.2009.02.083

Cited by 13 publications

(14 citation statements)

References 41 publications

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“…The latter behaviour seems to have been directing pv12 and pv38 evolution, highlighting high conservation at both intra- and inter-species level due to the influence of negative selection exerted on s48/45 domains which are important for red blood cell recognition [30]. Although antigens having low genetic diversity are usually not immunogenic [83] nor do they induce protection-inducing responses [84], some limited polymorphism antigens have been shown to be able to induce immunogenicity and protection [85]. Therefore, pv12 and pv38 (or their s48/45 domains) should be evaluated regarding vaccine development because immune responses against 6-Cys family antigens appear to be directed against structural epitopes in s48/45 domains [86-88], blocking such domains should prevent invasion [30,88] and being highly conserved and having a functional constraint, allele-specific immune responses are thus avoided.…”

Section: Resultsmentioning

confidence: 99%

Low genetic diversity and functional constraint in loci encoding Plasmodium vivax P12 and P38 proteins in the Colombian population

Forero-Rodríguez

Garzón‐Ospina

Patarroyo

2014

Malar J

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BackgroundPlasmodium vivax is one of the five species causing malaria in human beings, affecting around 391 million people annually. The development of an anti-malarial vaccine has been proposed as an alternative for controlling this disease. However, its development has been hampered by allele-specific responses produced by the high genetic diversity shown by some parasite antigens. Evaluating these antigens’ genetic diversity is thus essential when designing a completely effective vaccine.MethodsThe gene sequences of Plasmodium vivax p12 (pv12) and p38 (pv38), obtained from field isolates in Colombia, were used for evaluating haplotype polymorphism and distribution by population genetics analysis. The evolutionary forces generating the variation pattern so observed were also determined.ResultsBoth pv12 and pv38 were shown to have low genetic diversity. The neutral model for pv12 could not be discarded, whilst polymorphism in pv38 was maintained by balanced selection restricted to the gene’s 5′ region. Both encoded proteins seemed to have functional/structural constraints due to the presence of s48/45 domains, which were seen to be highly conserved.ConclusionsDue to the role that malaria parasite P12 and P38 proteins seem to play during invasion in Plasmodium species, added to the Pv12 and Pv38 antigenic characteristics and the low genetic diversity observed, these proteins might be good candidates to be evaluated in the design of a multistage/multi-antigen vaccine.

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Section: Resultsmentioning

confidence: 99%

Low genetic diversity and functional constraint in loci encoding Plasmodium vivax P12 and P38 proteins in the Colombian population

Forero-Rodríguez

Garzón‐Ospina

Patarroyo

2014

Malar J

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“…Indeed, scientific publications resulting from research conducted by FIDIC indicate that A. nancymaae and A. nigriceps have been used by them. All these publications have been reported to Corpoamazonia [e.g., Cardenas et al, 2005;Curtidor et al, 2007;Daubenberger et al, 2007;Patarroyo et al, 2006;Rojas-Caraballo et al, 2009;Suárez et al, 2011]. Ruiz-Garcia et al [2013] conducted a molecular genetics analysis of mtDNA COII gene sequences reporting genetic evidence of the illegal trade conducted by FIDIC.…”

Section: Discussionmentioning

confidence: 99%

Research and in situ conservation of owl monkeys enhances environmental law enforcement at the Colombian‐Peruvian border

Maldonado¹,

Peck

2014

American J Primatol

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This study reports on impacts of illegal trade in owl monkeys (Aotus nancymaae, A. vociferans) for the biomedical research market in the Colombian‐Peruvian Amazonian border. Through freedom of information requests and interviews with hunters we found that 912 owl monkeys, including A. nancymaae captured in Peru, were trapped over a 3‐month period in 2012 to supply a malaria research facility based in Leticia, Colombia, which had trapping permits for the use of only 800 A. vociferans annually yet experimentation took place using A. nancymaae. High levels of extraction in Peru have had population‐level impacts with significantly lower densities of Aotus spp. (3–24 individuals/km2) compared to Colombian sites with low hunting pressure (26–44 individuals/km2). Post‐experimental release of this species in Colombian territory has created a new distribution whose status and impacts on resident populations of A. vociferans remain unknown. The trapping method has also had environmental impact, with loss of over 65,000 trees (including sleeping sites), annually. As Aotus species are registered under the Convention of International Trade in Endangered Species of Wild Fauna and Flora (CITES) Appendix II, international trade requires official permission and evidence that extraction does not impact wild populations. However, no official records exist and CITES legislation has failed, due principally to a lack of appropriate monitoring by national authorities responsible for compliance. Of further concern is that we had previously documented and reported the illegal trade to the appropriate governmental authorities yet still no action was taken—as demonstrated by the continuing trade in 2013. Enforcement eventually occurred when a non‐governmental organization initiated legal action against organizations responsible. A successful second instance ruling by the Colombian State's Council in 2013 revoked trapping permits. Using the trade in owl monkeys as a case study we consider implementation, compliance, and enforcement of CITES in the border area to identify mechanisms to improve enforcement of environmental legislation. Am. J. Primatol. 76:658–669, 2014. © 2014 Wiley Periodicals, Inc.

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“…Of the 151 high immunogenic P. vivax proteins, only 3 proteins (ETRAMP11.2, Pv34 and SUB1) have been identified as immunogenic proteins in previous studies, 10,11,32,33 and 2 proteins (RAP2 and MSP4) were considered as potential targets of host immunity to vivax malaria. 34,35 Other proteins have not previously been described as immunologically reactive (Tables S1 and S2, ESI †). Forty of the 151 most immunoreactive proteins were considered as biomarkers for serodiagnosis, and 18 were proteins recognized by malaria serum samples with the area under the receiver operating characteristics (ROCs) curve (AUC) more than 0.95 (Table 1) (Fig.…”

Section: Immunomics Profiles Of the P Vivax Blood Stage Protein Micrmentioning

confidence: 99%

An immunomics approach for the analysis of natural antibody responses to Plasmodium vivax infection

Chen

Wang

et al. 2015

Mol. BioSyst.

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High throughput immunomics is a powerful platform to discover potential targets of host immunity and develop diagnostic tests for infectious diseases. We screened the sera of Plasmodium vivax-exposed individuals to profile the antibody response to blood-stage antigens of P. vivax using a P. vivax protein microarray. A total of 1936 genes encoding the P. vivax proteins were expressed, printed and screened with sera from P. vivax-exposed individuals and normal subjects. Total of 151 (7.8% of the 1936 targets) highly immunoreactive antigens were identified, including five well-characterized antigens of P. vivax (ETRAMP11.2, Pv34, SUB1, RAP2 and MSP4). Among the highly immunoreactive antigens, 5 antigens were predicted as adhesins by MAAP, and 11 antigens were predicted as merozoite invasion-related proteins based on homology with P. falciparum proteins. There are 40 proteins that have serodiagnostic potential for antibody surveillance. These novel Plasmodium antigens identified provide the clues for understanding host immune response to P. vivax infection and the development of antibody surveillance tools.

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Immunogenicity and protection-inducing ability of recombinant Plasmodium vivax rhoptry-associated protein 2 in Aotus monkeys: A potential vaccine candidate

Cited by 13 publications

References 41 publications

Low genetic diversity and functional constraint in loci encoding Plasmodium vivax P12 and P38 proteins in the Colombian population

Low genetic diversity and functional constraint in loci encoding Plasmodium vivax P12 and P38 proteins in the Colombian population

Research and in situ conservation of owl monkeys enhances environmental law enforcement at the Colombian‐Peruvian border

An immunomics approach for the analysis of natural antibody responses to Plasmodium vivax infection

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