Immunogenicity and Contraceptive Efficacy of <i><scp>Escherichia</scp> coli</i>‐Expressed Recombinant Porcine Zona Pellucida Proteins

Gupta, Neha; Chakrabarti, Krishanu; Prakash, Krishna; Wadhwa, Neerja; Gupta, Tripti; Gupta, Satish Kumar

doi:10.1111/aji.12095

Cited by 16 publications

(36 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Other studies on white-tailed deer and feral horses have reported that treatment with ZP vaccines does not affect time budget, social behaviour and body condition (Miller et al 2001;Hernandez et al 2006;Ransom et al 2010). The incidence of ovarian pathologies was significantly reduced when purified PZP proteins were used in vaccine constructs (Gupta et al 2013).…”

Section: Immunocontraceptive Vaccinesmentioning

confidence: 88%

“…Porcine ZP (PZP) immunocontraceptive vaccines, derived from ZP isolated from pig ovaries, have been effective in many ungulate species, monkeys, seals, bears and marsupials, but not in rodents, cats, dogs and wild pigs (Eade et al 2009;Kitchener et al 2009;Kirkpatrick et al 2009Kirkpatrick et al , 2011 McLaughlin and Aitken 2011; Table 1). However, recently formulated recombinant PZP3 and PZP4 vaccines, delivered in three injectable doses, caused infertility in up to 89% mice, depending on the formulation type (Gupta et al 2013).…”

Section: Immunocontraceptive Vaccinesmentioning

confidence: 99%

“…Fusion protein technology has also been used to produce a plasmid-DNA vaccine encoding GnRH; injection with this vaccine caused a significant reduction in fertility in both male and female mice (Mus musculus; (Khan et al 2008). To overcome the lack of availability of the purified native ZP glycoproteins obtained from ovaries of slaughtered pigs, porcine ZP3 and ZP4 were expressed in Escherichia coli; immunisation with these recombinant proteins significantly decreased fertility in laboratory mice and dogs (Gupta et al , 2013.…”

Section: Immunocontraceptive Vaccinesmentioning

confidence: 99%

See 2 more Smart Citations

Fertility control to mitigate human–wildlife conflicts: a review

2014

View full text Add to dashboard Cite

Abstract. As human populations grow, conflicts with wildlife increase. Concurrently, concerns about the welfare, safety and environmental impacts of conventional lethal methods of wildlife management restrict the options available for conflict mitigation. In parallel, there is increasing interest in using fertility control to manage wildlife. The present review aimed at analysing trends in research on fertility control for wildlife, illustrating developments in fertility-control technologies and delivery methods of fertility-control agents, summarising the conclusions of empirical and theoretical studies of fertility control applied at the population level and offering criteria to guide decisions regarding the suitability of fertility control to mitigate human-wildlife conflicts. The review highlighted a growing interest in fertility control for wildlife, underpinned by increasing numbers of scientific studies. Most current practical applications of fertility control for wild mammals use injectable single-dose immunocontraceptive vaccines mainly aimed at sterilising females, although many of these vaccines are not yet commercially available. One oral avian contraceptive, nicarbazin, is commercially available in some countries. Potential new methods of remote contraceptive delivery include bacterial ghosts, virus-like particles and genetically modified transmissible and non-transmissible organisms, although none of these have yet progressed to field testing. In parallel, new species-specific delivery systems have been developed. The results of population-level studies of fertility control indicated that this approach may increase survival and affect social and spatial behaviour of treated animals, although the effects are species-and context-specific. The present studies suggested that a substantial initial effort is generally required to reduce population growth if fertility control is the sole wildlife management method. However, several empirical and field studies have demonstrated that fertility control, particularly of isolated populations, can be successfully used to limit population growth and reduce human-wildlife conflicts. In parallel, there is growing recognition of the possible synergy between fertility control and disease vaccination to optimise the maintenance of herd immunity in the management of wildlife diseases. The review provides a decision tree that can be used to determine whether fertility control should be employed to resolve specific human-wildlife conflicts. These criteria encompass public consultation, considerations about animal welfare and feasibility, evaluation of population responses, costs and sustainability.

show abstract

Section: Immunocontraceptive Vaccinesmentioning

confidence: 88%

Section: Immunocontraceptive Vaccinesmentioning

confidence: 99%

Section: Immunocontraceptive Vaccinesmentioning

confidence: 99%

See 1 more Smart Citation

Fertility control to mitigate human–wildlife conflicts: a review

2014

View full text Add to dashboard Cite

show abstract

“…Immunization of female mice with E. coliexpressed recombinant porcine ZP3 and ZP4 with or without promiscuous T cell epitopes of either TT or bovine RNase led to the generation of high antibody titers with concomitant decrease in fertility. 109 In general, fusion proteins with promiscuous T cell epitopes generated higher antibody titers as well as contraceptive efficacy as compared with recombinant porcine ZP3 and ZP4 without promiscuous T cell epitope. The litter size in the immunized animals was significantly decreased as compared with the control group.…”

Section: Potential Of Recombinant Zp Proteins For Inhibiting Fertilitymentioning

confidence: 93%

“…The litter size in the immunized animals was significantly decreased as compared with the control group. 109 Immunization of female marmoset (Callithrix jacchus) with the recombinant human ZP3 expressed in Chinese Hamster Ovary (CHO) cells induced long-term infertility. 110 Immunized animals showed ovarian pathology associated with the depletion of primordial follicles.…”

Section: Potential Of Recombinant Zp Proteins For Inhibiting Fertilitymentioning

confidence: 99%

Milestones in contraceptive vaccines development and hurdles in their application

Gupta

Shrestha

Minhas

2013

Human Vaccines & Immunotherapeutics

View full text Add to dashboard Cite

Novel Sperm and Gonadotropin‐releasing Hormone‐based Recombinant Fusion Protein: Achievement of 100% Contraceptive Efficacy by Co‐immunization of Male and Female Mice

Minhas

Shrestha

Wadhwa

et al. 2016

Molecular Reproduction Devel

Self Cite

View full text Add to dashboard Cite

Improvements in long-term female contraception can be achieved by vaccinating with sperm-derived proteins. Here, recombinant proteins comprising either (i) N- (amino acid residues 1-80) or C- (amino acid residues 76-126) terminal fragments of mouse sperm protein 17 (Sp17) fused to the promiscuous T non-B cell epitope of tetanus toxoid (TT), amino acid residues 830-844 followed by di-lysine linker (KK) (TT-KK-Sp17 or TT-KK-Sp17 , respectively) or (ii) mouse equatorin (amino acid residues 21-185) fused to the T non-B cell epitope of bovine RNase (amino acid residues 94-104) were expressed in Escherichia coli. Immunization of female FVB/J mice, using alum as an adjuvant, led to the generation of high antibody titers against the above proteins. Antibodies against both N- and C-terminal fragments of Sp17 reacted with the entire capacitated mouse spermatozoa, whereas those against equatorin reacted exclusively with the equatorial region. Despite the reactivity of all immune sera, only sera from mice immunized with TT-KK-Sp17 and TT-KK-Sp17 significantly reduced mouse in vitro fertilization. Mating studies of the immunized females with un-immunized male mice revealed the highest infertility in the TT-KK-Sp17 -immunized group. In an attempt to further boost the immune response, the C-terminal fragment of Sp17 was expressed as fusion protein with a tandem repeat of gonadotropin-releasing hormone (GnRH) (Sp17 -GnRH ). Immunization of both male and female mice with Sp17 -GnRH led to higher contraceptive efficacy compared to mice immunized with TT-KK-Sp17 . Interestingly, mating studies wherein partners were both immunized with Sp17 -GnRH showed a complete failure of female mice to conceive. Thus, immunization of both males and females with Sp17 -GnRH has the potential to increase contraceptive efficacy. Mol. Reprod. Dev. 83: 1048-1059, 2016. © 2016 Wiley Periodicals, Inc.

show abstract

Immunogenicity and Contraceptive Efficacy of Escherichia coli‐Expressed Recombinant Porcine Zona Pellucida Proteins

Abstract: Availability of recombinant porcine proteins will be useful in the development of contraceptive vaccine.

Cited by 16 publications

References 43 publications

Fertility control to mitigate human–wildlife conflicts: a review

Fertility control to mitigate human–wildlife conflicts: a review

Milestones in contraceptive vaccines development and hurdles in their application

Novel Sperm and Gonadotropin‐releasing Hormone‐based Recombinant Fusion Protein: Achievement of 100% Contraceptive Efficacy by Co‐immunization of Male and Female Mice

Contact Info

Product

Resources

About