Immunogenic response of the venoms of fer-de-lance, Bothrops atrox asper, and la cascabella, Crotalus durissus durissus, following photooxidative detoxification

Kocholaty, Walter F.; Goetz, Joyce C.; Ashley, Billy D.; Billings, Thomas A.; Ledford, Edith B.

doi:10.1016/0041-0101(68)90082-2

Cited by 16 publications

(5 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In order to overcome these difficulties, several methods of detoxification have been studied in order to minimize the alterations caused by the venom (14,16,19,20,22,23,25,28,44). In addition, the researchers look for alternatives of antibodies production with high quality and the highest seric levels.…”

Section: Discussionmentioning

confidence: 99%

“…In regard to the techniques used in the toxoids preparation for antivenin preparation, innumerous works, in which chemical and/or physical agents were used for this purpose, are found. Among the chemical agents are: carboximetyl cellulose (28): photoxidation in the presence of methylene blue (25); chelating agents (20); formalin (14); iodine (16); glutaraldehide (22); and others. The principal physical agents are: xray radiation (19), ultraviolet radiation (44), heat (14), and gamma radiation (23).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Immunization with native and cobalt 60-irradiated Crotalus durissus terrificus venom in swiss mice: assessment of the neutralizing potency of antisera

Ferreira

Nascimento

Martı́nez

et al. 2005

J. Venom. Anim. Toxins incl. Trop. Dis

View full text Add to dashboard Cite

ELISA was used to evaluate, follow, and compare the humoral immune response of Swiss mice during hyperimmunization with natural and Cobalt 60-irradiated (60Co) Crotalus durissus terrificus venom. Potency and neutralization were evaluated by in vitro challenges. After hyperimmunization, immunity was observed by "in vivo" challenge and the side effects were assessed. The animals immunization with one LD50 of the venom was on days one, 15, 21, 30, and 45, when blood samples were collected; the challenges occurred on the 60th day. Results showed that ELISA was efficient in evaluating, following, and comparing mouse immune response during hyperimmunization. Serum titers produced with natural venom were similar to those produced with irradiated venom. Immunogenic capacity was maintained after 60Co irradiation. Serum produced from Crotalus durissus terrificus irradiated venom showed higher potency and neutralization capacity than that from natural venom. All antibodies were able to neutralize five LD50 from these venoms. Clinical alterations were minimum during hyperimmunization with irradiated venom

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Immunization with native and cobalt 60-irradiated Crotalus durissus terrificus venom in swiss mice: assessment of the neutralizing potency of antisera

Ferreira

Nascimento

Martı́nez

et al. 2005

J. Venom. Anim. Toxins incl. Trop. Dis

View full text Add to dashboard Cite

show abstract

“…SAWAI et al (1967) reported that the venoms of Crotalus atrox and C. durissus were detoxified by DHTA. KOCHOLATY et al (1968) noted that photo-oxidation of C. durissus venom resulted in detoxification of the venom. Immunization of rabbits with the toxoid ofthe venom plus the y-globulin isolated from the immunized animals gave a very high degree of protection.…”

Section: Comparison Of Antihemorrhagic Antinecrotic and Antilethal mentioning

confidence: 97%

Vaccination Against Snake Bite Poisoning

Sawai¹

1979

Snake Venoms

View full text Add to dashboard Cite

“…Furthermore, a comparative study in birds showed that species that accumulated more mitochondrial mutations have darker testes, potentially as an adaptation to protect sperm against oxidative stress. Similarly, in snakes, melanin prevents venom degradation by UV light (Kocholaty et al., ,b; Pough et al., ).…”

Section: Physiological Roles Of Internal Melaninmentioning

confidence: 99%

Evolutionary and biomedical consequences of internal melanins

Dubey

Roulin

2014

Pigment Cell Melanoma Res

View full text Add to dashboard Cite

Summary The adaptive function of melanin located in the integument is well known. Although pigments are also deposited in various internal organs, their function is unclear. A review of the literature revealed that ‘internal melanin’ protects against parasites, pollutants, low temperature, oxidative stress, hypoxemia and UV light, and is involved in the development and function of organs. Importantly, several studies have shown that the amount of melanin deposited on the external body surface is correlated with the amount located inside the body. This finding raises the possibility that internal melanin plays more important physiological roles in dark than light‐colored individuals. Internal melanin and coloration may therefore not evolve independently. This further emphasizes the major role played by indirect selection in evolutionary processes.

show abstract

Immunogenic response of the venoms of fer-de-lance, Bothrops atrox asper, and la cascabella, Crotalus durissus durissus, following photooxidative detoxification

Cited by 16 publications

References 4 publications

Immunization with native and cobalt 60-irradiated Crotalus durissus terrificus venom in swiss mice: assessment of the neutralizing potency of antisera

Immunization with native and cobalt 60-irradiated Crotalus durissus terrificus venom in swiss mice: assessment of the neutralizing potency of antisera

Vaccination Against Snake Bite Poisoning

Evolutionary and biomedical consequences of internal melanins

Contact Info

Product

Resources

About