Immunogenic Response of Brucella canisvirB10 and virB11 Mutants in a Murine Model

Resendiz, Gabriela Palomares; Arellano-Reynoso, Beatriz; Hernández‐Castro, Rigoberto; Tenorio-Gutiérrez, Víctor; Salas-Téllez, E.; Suárez-Güemes, Francisco; Díaz-Aparicio, Efrén

doi:10.3389/fcimb.2012.00035

Cited by 17 publications

(17 citation statements)

References 17 publications

(28 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…2A, inset). In agreement with a previous report (44), the B. canis virB10 mutant was already rapidly eliminated from all organs at 6 weeks postinfection (p.i.) and was not recovered from bone marrow or observed inside phagocytic cells (Fig.…”

Section: B Canis-infected Dogs Fail To Show Signs Of Sepsis or Clinisupporting

confidence: 93%

“…with 10 7 CFU of B. canis-GFP. As expected, the spleen replication curve profile achieved by B. canis-GFP was consistent with previous reports (44) (Fig. 2A).…”

Section: B Canis-infected Dogs Fail To Show Signs Of Sepsis or Clinisupporting

confidence: 92%

“…The milder inflammation of the target organs and the smaller amounts of IFN-␥ induced during B. canis infections of mice are in agreement with this proposal. This claim is also endorsed by works demonstrating rather low cytokine induction, even with heat-killed B. canis or B. canis ⌬virB mutant bacteria (44,55); absence of macrophage activation (25); and the lower reactive oxygen species response in B. canis-infected humans than in those infected with smooth Brucella species (56)(57)(58).…”

Section: Discussionmentioning

confidence: 89%

“…The relevance of the virB operon for B. canis virulence has been demonstrated before in the mouse model (44), but its participation in the intracellular lifestyle of this species has not. As is the case with other Brucella species, this system is an essential component of the achievement of successful intracellular replication of B. canis within the endoplasmic reticulum of host cells.…”

Section: Discussionmentioning

confidence: 93%

See 3 more Smart Citations

Brucella canis Is an Intracellular Pathogen That Induces a Lower Proinflammatory Response than Smooth Zoonotic Counterparts

et al. 2015

View full text Add to dashboard Cite

e Canine brucellosis caused by Brucella canis is a disease of dogs and a zoonotic risk. B. canis harbors most of the virulence determinants defined for the genus, but its pathogenic strategy remains unclear since it has not been demonstrated that this natural rough bacterium is an intracellular pathogen. Studies of B. canis outbreaks in kennel facilities indicated that infected dogs displaying clinical signs did not present hematological alterations. A virulent B. canis strain isolated from those outbreaks readily replicated in different organs of mice for a protracted period. However, the levels of tumor necrosis factor alpha, interleukin-6 (IL-6), and IL-12 in serum were close to background levels. Furthermore, B. canis induced lower levels of gamma interferon, less inflammation of the spleen, and a reduced number of granulomas in the liver in mice than did B. abortus. When the interaction of B. canis with cells was studied ex vivo, two patterns were observed, a predominant scattered cell-associated pattern of nonviable bacteria and an infrequent intracellular replicative pattern of viable bacteria in a perinuclear location. The second pattern, responsible for the increase in intracellular multiplication, was dependent on the type IV secretion system VirB and was seen only if the inoculum used for cell infections was in early exponential phase. Intracellular replicative B. canis followed an intracellular trafficking route undistinguishable from that of B. abortus. Although B. canis induces a lower proinflammatory response and has a stealthier replication cycle, it still displays the pathogenic properties of the genus and the ability to persist in infected organs based on the ability to multiply intracellularly. Brucellosis is a disease of animals and humans caused by members of the genus Brucella. Zoonotic species such as Brucella melitensis, Brucella abortus, and Brucella suis are facultative extracellular-intracellular stealthy pathogens that are able to overcome innate immunity at early times of infection (1-3) and at specific stages of adaptive immunity (4, 5). In addition to influencing the immune response, these Brucella species are able to circumvent the killing action of professional and nonprofessional phagocytes, transit within phagocytic vacuoles, and replicate extensively within the endoplasmic reticulum of cells (6). These properties allow the bacterium to spread throughout the reticuloendothelial system and promote chronic infection (3).There are other Brucella species that are also relevant pathogens; however, their infective strategies remain unclear and are not in tune with the solid results accepted for the previously mentioned zoonotic brucellae. Among these are Brucella canis, the etiological agent of brucellosis in dogs and a zoonotic pathogen (7). This pathogen induces a subclinical infection that may remain undiagnosed for protracted periods (8-10). B. canis invades the conjunctiva or the oronasal system or penetrates through the venereal route. Then it is distributed to different organs...

show abstract

Section: B Canis-infected Dogs Fail To Show Signs Of Sepsis or Clinisupporting

confidence: 93%

“…with 10 7 CFU of B. canis-GFP. As expected, the spleen replication curve profile achieved by B. canis-GFP was consistent with previous reports (44) (Fig. 2A).…”

Section: B Canis-infected Dogs Fail To Show Signs Of Sepsis or Clinisupporting

confidence: 92%

Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 93%

See 2 more Smart Citations

Brucella canis Is an Intracellular Pathogen That Induces a Lower Proinflammatory Response than Smooth Zoonotic Counterparts

et al. 2015

View full text Add to dashboard Cite

show abstract

“…canis, which elicited a protective immunity in mice (Palomares-Resendiz et al, 2012) but vaccination with whole-cell vaccines would interfere with current diagnostic test.…”

Section: Introductionmentioning

confidence: 99%

Polymeric antigen BLSOmp31 in aluminium hydroxide induces serum bactericidal and opsonic antibodies against Brucella canis in dogs

Clausse

Díaz

Pardo

et al. 2017

Veterinary Immunology and Immunopathology

View full text Add to dashboard Cite

M., Polymeric antigen BLSOmp31 in aluminium hydroxide induces serum bactericidal and opsonic antibodies against Brucella canis in dogs.Veterinary Immunology and Immunopathology http://dx.doi.org/10. 1016/j.vetimm.2016.11.004 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

show abstract

Comparative proteomics analysis of host cells infected with Brucella abortusA19

Jin

Pan

et al. 2014

Electrophoresis

View full text Add to dashboard Cite

We carried out a proteomic analysis of THP-1-derived macrophages with and without Brucella abortus A19 (B. abortus A19) infection in order to study the cellular responses to B. abortus A19. The proteins were analyzed at different time points after infection with 2DE followed by MALDI-TOF/TOF identification. Comparative analysis of multiple 2DE gels revealed that the majority of changes in protein abundance appeared between 48 and 96 h after infection. MS identified 44 altered proteins, including 20 proteins increased in abundance and 24 proteins decreased in abundance, which were found to be involved in cytoskeleton, signal transduction, energy metabolism, host macromolecular biosynthesis, and stress response. Moreover, 22 genes corresponding to the altered proteins were quantified by real-time RT-PCR to examine the transcriptional profiles between infected and uninfected THP-1-derived macrophages. Finally, we mapped the altered pathways and networks using ingenuity pathway analysis, which suggested that the altered protein species were heavily favored germ cell-Sertoli cell junction signaling as the primary pathway. Furthermore, mechanisms of viral exit from host cell and macrophage stimulating protein-recepteur d'origine nantais signaling appeared to be major pathways modulated in infected cells. This study effectively provides useful dynamic protein-related information concerning B. abortus infection in macrophages.

show abstract

Immunogenic Response of Brucella canisvirB10 and virB11 Mutants in a Murine Model

Cited by 17 publications

References 17 publications

Brucella canis Is an Intracellular Pathogen That Induces a Lower Proinflammatory Response than Smooth Zoonotic Counterparts

Brucella canis Is an Intracellular Pathogen That Induces a Lower Proinflammatory Response than Smooth Zoonotic Counterparts

Polymeric antigen BLSOmp31 in aluminium hydroxide induces serum bactericidal and opsonic antibodies against Brucella canis in dogs

Comparative proteomics analysis of host cells infected with Brucella abortusA19

Contact Info

Product

Resources

About