Immunogenic Proteins of Capripox Virus: Potential Applications in Diagnostic/Prophylactic Developments

Development of in-house ELISA for detection of antibodies against lumpy skin disease virus in cattle and assessment of its performance using a bayesian approach

“…A previous genomic study of LSDV indicated that the LSDV was closely related to other members of the Chordopoxvirinae rather than members in the same genus. The data would also suggest that the findings indicate that this group of genes was responsible for viral host range and virulence, as well as immunogenicity [ 2 , 34 ]. However, certain candidate immunogenic proteins have been recommended for the ELISA, namely the p32 protein of Capripoxvirus [ [31] , [32] , [33] , [34] ].…”

Designing and Modelling of Trivalent Chimeric Vaccine for Capripoxvirus: Lumpy Skin Disease, Sheep Pox and Goat Pox by Immunoinformatics Approach

“…CaPVs causes a highly contagious disease in cattle, goats, and sheep and its endemicity in many countries causes signi cant economic losses (Chervyakova et al 2016;Kushwaha et al 2019). The outbreak of Capripox viral disease can be prevented and eradicated by vaccination, culling of the infected and exposed animals from the herd, proper methods of disposal of carcasses, regular cleaning and hygienic maintenance of animal sheds, disinfection of the animal premises, and proper insect control measures (Tuppurainen et al 2017).…”

“…This F13L is the most abundant protein among EEV membrane proteins and, have a molecular weight of ~ 37kDa with a palmitoylated component (Grosenbach, Ulaeto, and Hruby 1997; Kushwaha et al 2019). The gene for F13L is homologous to the B2L gene of Orf Virus and is potentially immunogenic and, it was observed that ORFV B2L can elicit neutralizing antibodies against LSD in mice ( The B5R protein of the vaccinia virus is reported to induce the neutralizing antibodies in infected animals (Hooper et al 2000;Hooper, Custer, and Thompson 2003;Law and Smith 2001) and the antisera produced against B5R protein is e cient in neutralizing EEV (Benhnia et al 2009).…”

“…The gene for F13L is homologous to the B2L gene of Orf Virus and is potentially immunogenic and, it was observed that ORFV B2L can elicit neutralizing antibodies against LSD in mice ( The B5R protein of the vaccinia virus is reported to induce the neutralizing antibodies in infected animals (Hooper et al 2000;Hooper, Custer, and Thompson 2003;Law and Smith 2001) and the antisera produced against B5R protein is e cient in neutralizing EEV (Benhnia et al 2009). The immunogenicity of GTPV homolog of B5R protein has been evaluated along with other immunogenic proteins (Kushwaha et al 2019;Zheng et al 2009). Among IMV (intracellular mature virion) proteins, P32, which is a homolog of the VACV H3L, is one of the most immunodominant proteins (~ 35kDa), located on the surface of IMV of all poxvirus members (Kushwaha et al 2019;Lin et al 2000).…”

“…The immunogenicity of GTPV homolog of B5R protein has been evaluated along with other immunogenic proteins (Kushwaha et al 2019;Zheng et al 2009). Among IMV (intracellular mature virion) proteins, P32, which is a homolog of the VACV H3L, is one of the most immunodominant proteins (~ 35kDa), located on the surface of IMV of all poxvirus members (Kushwaha et al 2019;Lin et al 2000). High titers of neutralizing antibodies were reported to be induced by the recombinant H3L protein in immunized mice (Bhanot et al 2009; Davies et al 2005).…”