Immunogenic protein profiling of pathogenic Escherichia coli strains isolated from infants with diarrhea in Quetta Balochistan

Zil-e-Huma,; Tareen, Abdul Malik; Samad, Abdul; Mustafa, Muhammad Zahid; Maryam, Maqsood; Rizwan, Sabeena; Akbar, Ali

doi:10.1016/j.jksus.2022.101883

Cited by 5 publications

(3 citation statements)

References 23 publications

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“…All five main pathotypes of diarrhoeagenic E. coli were detected in the area under study. This is in support of the assertion that there are five main pathotypes of diarrhoeagenic E. coli (Tareen et al, 2022;Redha et al, 2022). The study observed that some of the study participants exhibited symptoms of diarrhoea while others did not show any signs of diarrhoea although they were positive for the pathotypes proved by the presence of the virulence factors (Tables 2 and 3).…”

Section: Discussionsupporting

confidence: 86%

Study of Escherichia coli as a Cause of Diarrhoea in the Ashanti Region of Ghana

Awuah,

Addo,

Ofori

2023

ESJ

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Even though diarrhoeagenic Escherichia coli (E. Coli) is accepted worldwide as essential diarrhoea-causing agent, especially among children, routinely they are not sought as a stool sample pathogen in many microbiological laboratories. The conventional biochemical means are not able to differentiate E. coli which is a normal flora in the gastrointestinal tract from the pathogenic strains. This research work sought to detect and identify diarrhoeagenic E.coli in the Ashanti region of Ghana by using conventional multiplex PCR. A convenient sampling method was employed to select a total of 502 patients from Ashanti Regional and St. Michael’s Hospitals for the study. In all, five pathotypes of diarrhoeagenic E.coli were detected with enterotoxigenic E. coli (ETEC) as the most frequent. The target genes considered for each group of diarrhoeagenic E. coli were eaeA (E. coli attaching- effacing) and bfpA (bundle forming pilus A) for enteropathogenic E. coli (EPEC), elt and Stla for enterotoxigenic E. coli (ETEC), ial for enteroinvasive E. coli (EIEC), CVD432 for enteroaggregative E. coli (EAEC), and hlyA for enterohaemorrhagic E. coli (EHEC). Generally, some of the participants (31.08% = n/N= 69/222) exhibited symptoms of diarrhoea while others did not (68.92% = n/N =153/222 ), although they were positive for the pathotypes. The virulence factors considered were seen as contributory factors to the symptomatic situations. Participants in the ≤5 and ≥ 42-year groups were seen to be more vulnerable to diarrhoea. Additionally, it was discovered that men were more susceptible to diarrhoea than women. The study therefore recommends more studies into the ≥ 42-year group not neglecting that of children. In addition, this current study suggests the routine utilization of molecular methods such as conventional multiplex PCR to identify and detect the pathotypes of E. coli causing diarrhoea.

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Section: Discussionsupporting

confidence: 86%

Study of Escherichia coli as a Cause of Diarrhoea in the Ashanti Region of Ghana

Awuah,

Addo,

Ofori

2023

ESJ

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“…E. coli is a Gram-negative bacterium and the most prevalent facultative anaerobic species in the gastrointestinal tract of humans and warm-blooded animals. While it is normally considered harmless, some strains can be highly pathogenic [ 23 , 24 ] and acquire pathogenic or virulence factors, making them medically important. They can cause a number of significant illnesses and severe infections both in humans and animals, such as bacteremia, wound infections, urinary tract infection, and gastrointestinal tract infections [ 9 , 24 , 25 ].…”

Section: Food-producing Animals As One Of the Potential Sources Of Fo...mentioning

confidence: 99%

Antimicrobial Resistance and Clonal Lineages of Escherichia coli from Food-Producing Animals

Silva

Pereira

et al. 2023

Antibiotics

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Escherichia coli are one of the most important pathogenic bacteria readily found in the livestock and widely studied as an indicator that carries drug-resistant genes between humans, animals, and the environment. The use of antimicrobials in the food chain, particularly in food-producing animals, is recognized as a significant contributor to the development and spread of antimicrobial resistance (AMR) and resistance genes can be transferred from the farm through the food-chain. The objective of this review is to highlight the background of the antimicrobials use in food-producing animals, more specifically, to study clonal lineages and the resistance profiles observed in E. coli, as well as in extended spectrum beta-lactamases (ESBL) producing E. coli, in a set of food-production animals with greater relevance in food consumption, such as pigs, poultry, cattle, fish farming and rabbits. Regarding the prevalence of ESBL-producing E. coli among farm animals, high-to-moderate prevalence was observed, and the highest resistance rates to tetracycline and ampicillin was detected in different farms in all geographic regions. Worldwide pandemic clones and high-risk zoonotic E. coli clones have been identified in most food-producing animals, and some of these clones are already disseminated in different niches, such as the environment and humans. A better understanding of the epidemiology of E. coli and ESBL-producing E. coli in livestock is urgently needed. Animal production is one of the major causes of the antibiotic resistance problem worldwide and a One Health approach is needed.

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“…Di antara masalah kesehatan global, diare adalah bagian dari persoalan kesehatan yang paling dominan karena dapat menyebabkan kematian. Kematian yang disebab oleh diare diperoleh 1,3 juta anak setiap tahun di seluruh dunia (Zil-e-Huma et al, 2022). ASI (Air Susu Ibu) sudah teruji bisa menghindari diare namun, masih ada ibu yang tidak menyusui anaknya sehingga balita tidak memperoleh ASI secara eksklusif, sedangkan angka morbiditas dan mortalitas penyakit ini tergolong besar.…”

Section: Pendahuluanunclassified

Risk of Diarrhea in Infants on Breastfeeding Exclusive and Non-Exclusive Breastfeeding

Irwan,

Katili,

Una

et al. 2023

JIKA

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Diarrheal disease is a significant risk factor for morbidity and mortality worldwide in children under five years of age. The study aimed to analyze the risk factors for diarrhea in infants with and without exclusive breastfeeding. The research approach used a case-control analysis with observational analytic methods. The population was all mothers who gave birth to babies aged 6-12 months. By determining the sample using the Lemeshow formula, the example was 152 babies with details of 76 cases and 76 controls. The results of the chi-square test showed (p-value= 0,000), which means that there was an effect of exclusive breastfeeding and non-exclusive breastfeeding with the incidence of diarrhea in infants with a value of OR=12,065, which means that mothers who did not breastfeed their babies 12,065 times more at risk of suffering from diarrhea higher inversely compared to mothers who exclusively breastfeed their babies. In conclusion, infants who are not exclusively breastfed are at risk of suffering from diarrhea than infants who are exclusively breastfed.

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Immunogenic protein profiling of pathogenic Escherichia coli strains isolated from infants with diarrhea in Quetta Balochistan

Cited by 5 publications

References 23 publications

Study of Escherichia coli as a Cause of Diarrhoea in the Ashanti Region of Ghana

Study of Escherichia coli as a Cause of Diarrhoea in the Ashanti Region of Ghana

Antimicrobial Resistance and Clonal Lineages of Escherichia coli from Food-Producing Animals

Risk of Diarrhea in Infants on Breastfeeding Exclusive and Non-Exclusive Breastfeeding

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