Immunogenic properties of SARS-CoV-2 inactivated by ultraviolet light

Gracheva, A. V.; Korchevaya, Ekaterina; Ammour, Yulia; Смирнова, Д. И.; Sokolova, Olga S.; Glukhov, Grigory; Moiseenko, Andrey; Zubarev, I. V.; Самойликов, Р. В.; Leneva, I. A.; Svitich, Oxana; Zverev, V. V.; Файзулоев, Е. Б.

doi:10.1007/s00705-022-05530-7

Cited by 10 publications

(9 citation statements)

References 39 publications

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“…Recent research reports that UV disinfection of SARS-CoV-2 halts infectivity while preserving its morphology and antigenic properties. 30 Since the morphology and antigenic properties did not change, this may suggest that changes in the genome may play a key role. Furthermore, recent research has been able to predict UV sensitivity based on the genome alone.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Exploring the Differences in the Response of SARS-CoV-2 Delta and Omicron to Ultraviolet Radiation

et al. 2023

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One method that can help slow the spread of coronaviruses is disinfection with UV light. The Delta and Omicron variants of the COVID-19 virus (SARS-CoV-2) have come to dominate the later stages of the pandemic due to their higher rates of transmission. In this work, it is shown that a 17% higher UV 254 dose is required for the disinfection of Delta and Omicron variants when compared to the ancestral strain of SARS-CoV-2. The UV 254 disinfection rate constants for SARS-CoV-2 and the Delta and Omicron variants were found to be 1.4 ± 0.3, 1.1 ± 0.2, and 1.1 ± 0.2 cm 2 /mJ, respectively. The rate constants of Delta and Omicron were statistically different from the ancestral strain of SARS-CoV-2 at the 95% confidence level based on at least three replicate experiments. It is suggested that the reason for this difference is the absence of repeating uracil (U) bases in the genome of the two variants. The UV 254 sensitivity of repeating pyrimidine bases is well-established. There are 2.6 and 3.7% fewer uracil triplets (UUU) in the Delta and Omicron variants, respectively, when compared to SARS-CoV-2. This difference in UV 254 sensitivity is relevant to a range of UV disinfection applications including upper-room disinfection, air handling equipment, aircraft sanitization, and others.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Exploring the Differences in the Response of SARS-CoV-2 Delta and Omicron to Ultraviolet Radiation

et al. 2023

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“…The genome inactivation was done upon a controlled UV treatment of the cultured virus sample. We chose a suitable UV exposure or dose that affected the viral genome without altering the structural integrity of the virus as reported by recent literature on the UV disinfection of SARS-CoV-2 . A similar inactivation approach has been applied by Petitjean et al for SARS-CoV-2, Peerboom et al for HSVs, and others for studying the binding of the virus particles to the membrane receptors and attachment factors. , We compared the results of the plaque assay using both live and UV-treated virus samples.…”

Section: Resultsmentioning

confidence: 99%

“…10 mL of viral supernatant was UV-inactivated using a UV chamber in a UV crosslinker at wavelength 254 nm for ∼4 min (Amersham Biosciences) set on the energy mode at 2700 × 100 μJ energy. For UV disinfection, we kept the power in the UV chamber within <500 μW/cm 2 , which can only damage the viral genome without any aberrant effect on the morphology of virions as also previously described by many researchers. ,, The UV-treated viral samples were concentrated using a 100 kDa concentrator to remove any free proteins. Inactivation was confirmed by the absence of plaque-forming units in the UV-treated samples.…”

Section: Methodsmentioning

confidence: 99%

SARS-CoV-2 Binding to Terminal Sialic Acid of Gangliosides Embedded in Lipid Membranes

et al. 2023

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Multiple recent reports indicate that the S protein of SARS-CoV-2 specifically interacts with membrane receptors and attachment factors other than ACE2. They likely have an active role in cellular attachment and entry of the virus. In this article, we examined the binding of SARS-CoV-2 particles to gangliosides embedded in supported lipid bilayers (SLBs), mimicking the cell membrane-like environment. We show that the virus specifically binds to sialylated (sialic acid (SIA)) gangliosides, i.e., GD1a, GM3, and GM1, as determined from the acquired single-particle fluorescence images using a time-lapse total internal reflection fluorescence (TIRF) microscope. The data of virus binding events, the apparent binding rate constant, and the maximum virus coverage on the ganglioside-rich SLBs show that the virus particles have a higher binding affinity toward the GD1a and GM3 compared to the GM1 ganglioside. Enzymatic hydrolysis of the SIA−Gal bond of the gangliosides confirms that the SIA sugar unit of GD1a and GM3 is essential for virus attachment to the SLBs and even the cell surface sialic acid is critical for the cellular attachment of the virus. The structural difference between GM3/GD1a and GM1 is the presence of SIA at the main or branched chain. We conclude that the number of SIA per ganglioside can weakly influence the initial binding rate of SARS-CoV-2 particles, whereas the terminal or more exposed SIA is critical for the virus binding to the gangliosides in SLBs.

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“…Real-time RT-PCR was performed as described earlier [29] . To detect viral RNA we used primers and the probe designed for the nucleocapsid N gene of the SARS-CoV-2 virus, proposed by Chan J. et al [30] .…”

Section: Methodsmentioning

confidence: 99%

“…Determination of antibodies to SARS-CoV-2 in hamster sera was performed using BioKit ELISA reagent kit (Bioservice, Russia) according to the instruction. Inactivation of SARS-CoV-2 by UV-light was performed as described earlier [29] . For use in the ELISA, a 45 ml centrifuged clarified UV-inactivated virus-containing supernatant (≥8.5 log10 TCID 50 /ml) was passed through a 100 kD Amikon MWCO centrifuge filter (Millipore, Ireland) at 4000 rpm.…”

Section: Methodsmentioning

confidence: 99%

Cold-adapted SARS-CoV-2 variants with different temperature sensitivity exhibit an attenuated phenotype and confer protective immunity

Файзулоев

Gracheva

Korchevaya

et al. 2023

Vaccine

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Immunogenic properties of SARS-CoV-2 inactivated by ultraviolet light

Cited by 10 publications

References 39 publications

Exploring the Differences in the Response of SARS-CoV-2 Delta and Omicron to Ultraviolet Radiation

Exploring the Differences in the Response of SARS-CoV-2 Delta and Omicron to Ultraviolet Radiation

SARS-CoV-2 Binding to Terminal Sialic Acid of Gangliosides Embedded in Lipid Membranes

Cold-adapted SARS-CoV-2 variants with different temperature sensitivity exhibit an attenuated phenotype and confer protective immunity

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