Immunogenic Properties of Rat Hepatoma Subcellular Fractions

Induction of immunity to chemically-induced rat tumours by cellular or soluble antigens

“…For example, Wolf and Avis (1970) indicated that the size of plasma membrane fragments is critical for the induction of immunity to a carcinogen-induced murine lymphoma and the greatest protection to viable tumour cell challenge was produced by immunisation with almost intact cell 'ghosts' rather than fragmented surface membranes. This was not found to be the case with one aminoazodye-induced rat hepatoma, even though the membrane fractions isolated were shown to retain tumour-specific antigenic activity (Price and Baldwin, 1974b).…”

Treatment of transplanted rat tumours with double-stranded RNA (BRL 5907). I. Influence of systemic and local administration

“…In addition, the ability of mixed inocula of tumour cells and ds-RNA to induce tumour specific immunity, and their suitability as a vaccine for active immunotherapy, have been assessed, particularly in comparison with previous studies with BCG and tumour cell mixed inocula (Baldwin and Pimm, 1973a . Hepatoma D23, induced by oral administration of 4-dimethylaminoazobenzene, is also immunogenic, producing resistance following graft excision to challenge with up to 5 x 105 tumour cells (Baldwin and Barker, 1967;Price and Baldwin, 1974). Mammary carcinoma AAF57 induced by repeated intraperitioneal injection of N-hydroxy-2-acetylaminofluorene lacks detectable immunogenicity, excision of subcutaneous grafts failing to elicit resistance to challenge with 1 x 103 cells, this being the minimum inoculum for growth in control rats (Baldwin and Embleton, 1974).…”

Preparation of Aminoazo Dye Induced Rat Hepatoma Membrane Fractions Retaining Tumour Specific Antigen

“…The objective of this investigation was to isolate membrane fractions which exhibit increased antigenic activity (as defined by an in vitro antigen assay) over other subcellular fractions prepared so that these techniques would provide material suitable for subsequent antigen isolation and characterization. The immunological characteristics of these tumouir membrane preparations are described in the second conmmunication (Price and Baldwin, 1974 Isolation of membranes by A-XII zonal centrifugation. Chopped hepatoma D23 tissue was passed 3 times through a tissue press (Harrison Type 1 tissue mincer) using coarse, medium and finally 60 mesh stainless steel grids.…”