Immunogenic Properties of a Soluble Tumor-Specific Transplantation Antigen Induced by Simian Virus 40

Drapkin, Mark S.; Appella, Ettore; Law, Lloyd W.

doi:10.1093/jnci/52.1.259

Cited by 74 publications

(29 citation statements)

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“…The lack of cross-reactions of mKSA with Adj PC-5, a plasma cell tumor of BALB/c mice was shown previously [12]. Meth-A, a methylcholanthrene-induced sarcoma and LSTRA, an M-MuLV-induced leu kemia, both of BALB/c strain mice do not immunize against mKSA nor does mKSA immunize against either of these neoplasms.…”

Section: Tumor-specific Transplantation Antigens-dna Virus (Sv40) Tstasupporting

confidence: 58%

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Histocompatibility Antigens and Tumor-Specific Transplantation Antigens

Appella¹,

Law²

1976

Pathobiology

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Section: Tumor-specific Transplantation Antigens-dna Virus (Sv40) Tstasupporting

confidence: 58%

“…1 for details). mKSA (Tu5) and mKSA (ASC) were equally effective as source material and thus share the same TSTA despite longcontinued passage in vitro and in vivo [12,26].…”

Section: Tumor-specific Transplantation Antigens-dna Virus (Sv40) Tstamentioning

confidence: 99%

Histocompatibility Antigens and Tumor-Specific Transplantation Antigens

Appella¹,

Law²

1976

Pathobiology

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“…cally against the growth of mKSA cells (17,19). The MSB cell line was established from MMuSV-induced tumor in C57BL/6 mice (20).…”

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confidence: 99%

Regulation of specific cell-mediated cytotoxic response against SV40-induced tumor associated antigens by depletion of suppressor T cells with cyclophosphamide in mice.

Glaser¹

1979

The Journal of Experimental Medicine

105

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When cyclophosphamide was administered to mice before immunization with syngeneic SV40 transformed cells, the specific immune response elicited, as was measured by in vitro 51Cr release assay was stronger and lasted longer when compared to the response generated in noncyclophosphamide-treated mice. The augmentation effect of the drug was dependent on cyclophosphamide concentration being optimal at 100 mg/kg and on the time of drug administration in relation to antigen immunization being optimal at 2 d before antigen administration. Transfer of T cells from normal syngeneic mice to drug-treated animals abolished the cyclophosphamide-induced augmentation of immune response. These results implied that cyclophosphamide sensitive T cells suppressed the in vivo generation of specific effector T cells against SV40-induced tumor-associated antigens.

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“…The SV AL/N cells were obtained by SV40 infection of early tissue culture transfer cells of the same AL/N line from which the T AL/N cells originated (Takemoto et al, 1968). The T AL/N cells were cloned, one clone (T AL/N C1 3) was infected with SV40, SV4O-transformed (T-antigen-positive) sister subclones 1 and 2, and untransfornied (T-antigennegative) sister subclones 5 and 6 were obtained (Coll, Luborsky and Mora, ms. submitted town University, Washington, D. C , SV40 TSTA activity, and has a 50% tumor dose (TD,,) of < lo3 cells by intraperitoneal (IP) injection (Drapkin et al, 1974). The BALB 3T3 clone A31 line, and the SV40-transformed derivative clonal line llA8 have been described (Smith et al, 1971); these cells were obtained from Dr. Todaro (National Cancer Institute, Bethesda, Md.).…”

Section: Cell Linesmentioning

confidence: 99%

Detergent solubilization and partial purification of tumor specific surface and transplantation antigens from SV40‐virus‐transformed mouse cells

Chang¹,

Pancake²,

Luborsky³

et al. 1977

Intl Journal of Cancer

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A solubilization technique employing 0.5% Triton X-100 was developed to obtain both SV40 virus (SV40)-induced tumor-specific surface antigen(s) (TSSA) from SV40-transformed mouse cells, as determined by a serum-mediated microcytolytic assay, and tumor-specific transplantation antigen(s) (TSTA), as determined by in invivo experiments. High yields (approximately 50%) of TSSA were obtained in whole-cell extracts and also after ammonium sulfate fractionation. Additional fractionation of a 30-50% ammonium sulfate fraction by gel exclusion chromatography on Sephadex G-150 resulted in two pooled fractions which contained TSSA activity. The first eluted close to the void volume, and the second in the 45,000 molecular weight region. The various TSSA active fractions were also active in vivo TSTA tests. Detergent solubilization provides a suitable technique to recover the SV40-induced antigens in good yield, and apparently in intact form.

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Immunogenic Properties of a Soluble Tumor-Specific Transplantation Antigen Induced by Simian Virus 40

Cited by 74 publications

References 0 publications

Histocompatibility Antigens and Tumor-Specific Transplantation Antigens

Histocompatibility Antigens and Tumor-Specific Transplantation Antigens

Regulation of specific cell-mediated cytotoxic response against SV40-induced tumor associated antigens by depletion of suppressor T cells with cyclophosphamide in mice.

Detergent solubilization and partial purification of tumor specific surface and transplantation antigens from SV40‐virus‐transformed mouse cells

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