Immunogenic characterization of vaccines based on Haemophilus parasuis Nagasaki strain, OmpP2, OmpP5 and OmpD15, in colostrum-deprived pigs experimentally challenged with the same strain

Álvarez-Estrada, Álvaro; Martínez-Martínez, Sonia; Gutiérrez-Martín, César B.; García-Iglesias, María-José; Pérez-Martínez, Claudia; Yubero-Delgado, Sheila; Guizzo, João Antônio; Frandoloso, Rafael; Ferri, Elías Fernando Rodríguez

doi:10.1016/j.rvsc.2018.07.009

Cited by 9 publications

(5 citation statements)

References 25 publications

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“…For example, with the usage of antibiotics, more antibiotic resistance genes have been conferred to G. parasuis due to the fact of its evolution [ 8 , 9 ]. Moreover, the actual protection effect of some candidate vaccines in pigs is still nonideal [ 10 , 11 ]. An earlier study has shown that 10% of all experimentally infected pigs were fully resistant, suggesting that there are differences in susceptibility to G. parasuis infection in pigs.…”

Section: Introductionmentioning

confidence: 99%

Assessment of the Macrophage Scavenger Receptor CD163 in Mediating Glaesserella parasuis Infection of Host Cells

Deng

Zhu

et al. 2023

Veterinary Sciences

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The macrophage CD163 surface glycoprotein is a member of the SRCR family class B, which has been identified as the key trigger in host–pathogen interactions, but its specific roles in sensing Glaesserella parasuis (G. parasuis) infection are largely unknown. Here, we investigated porcine CD163 in mediating the adhesion and immune response of G. parasuis using in vitro host–bacteria interaction models. CD163-overexpressing Chinese hamster ovary K1 cells (CHO-K1) showed obvious subcellular localization in the cytoplasm, especially in the cytomembrane. Although detection using scanning electron microscopy (SEM) confirmed the bacterial adhesion, there was no significant difference in the adhesion of G. parasuis to CHO-K1 cells between the presence and absence of CD163. In addition, similar results were observed in 3D4/21 cells. Meanwhile, bindings of G. parasuis to nine synthetic peptides, the bacterial binding motifs within SRCR domains of CD163, were weak based on a solid-phase adhesion assay and agglutination assay. Moreover, CD163 had no effect on the expression of G. parasuis-induced inflammatory cytokines (IL-6, INF-γ, IL-10, IL-4 and TGF-β) in CHO-K1 cells. In conclusion, these findings indicate that porcine CD163 plays a minor role in sensing G. parasuis infection.

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Section: Introductionmentioning

confidence: 99%

Assessment of the Macrophage Scavenger Receptor CD163 in Mediating Glaesserella parasuis Infection of Host Cells

Deng

Zhu

et al. 2023

Veterinary Sciences

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“…For example, pigs immunized with VtaA did not produce antibodies against the F6 fragments ( Correa-Fiz et al., 2017 ). In addition, some studies used rOmpP2 to immunize colostrum-deprived pigs, showing that it could only delay death in experimental pigs after a challenge experiment with lethal doses of strains, and not allow the hosts to survive as observed in the corresponding mouse experiment ( Alvarez-Estrada et al., 2018 ). Although sera positive for all epitopes screened by mouse-derived mAbs were present in swine herds, there were some differences between the two species.…”

Section: Discussionmentioning

confidence: 99%

Screening of linear B-cell epitopes and its proinflammatory activities of Haemophilus parasuis outer membrane protein P2

Nan

Peng

et al. 2023

Front. Cell. Infect. Microbiol.

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Haemophilus parasuis is a commensal organism of the upper respiratory tract of pigs, but virulent strains can cause Glässer’s disease, resulting in significant economic losses to the swine industry. OmpP2 is an outer membrane protein of this organism that shows considerable heterogeneity between virulent and non-virulent strains, with classification into genotypes I and II. It also acts as a dominant antigen and is involved in the inflammatory response. In this study, 32 monoclonal antibodies (mAbs) against recombinant OmpP2 (rOmpP2) of different genotypes were tested for reactivity to a panel of OmpP2 peptides. Nine linear B cell epitopes were screened, including five common genotype epitopes (Pt1a, Pt7/Pt7a, Pt9a, Pt17, and Pt19/Pt19a) and two groups of genotype-specific epitopes (Pt5 and Pt5-II, Pt11/Pt11a, and Pt11a-II). In addition, we used positive sera from mice and pigs to screen for five linear B-cell epitopes (Pt4, Pt14, Pt15, Pt21, and Pt22). After porcine alveolar macrophages (PAMs) were stimulated with overlapping OmpP2 peptides, we found that the epitope peptides Pt1 and Pt9, and the loop peptide Pt20 which was adjacent epitopes could all significantly upregulated the mRNA expression levels of IL-1α, IL-1β, IL-6, IL-8, and TNF-α. Additionally, we identified epitope peptides Pt7, Pt11/Pt11a, Pt17, Pt19, and Pt21 and loop peptides Pt13 and Pt18 which adjacent epitopes could also upregulate the mRNA expression levels of most proinflammatory cytokines. This suggested that these peptides may be the virulence-related sites of the OmpP2 protein, with proinflammatory activity. Further study revealed differences in the mRNA expression levels of proinflammatory cytokines, including IL-1β and IL-6, between genotype-specific epitopes, which may be responsible for pathogenic differences between different genotype strains. Here, we profiled a linear B-cell epitope map of the OmpP2 protein and preliminarily analyzed the proinflammatory activities and effects of these epitopes on bacterial virulence, providing a reliable theoretical basis for establishing a method to distinguish strain pathogenicity and to screen candidate peptides for subunit vaccines.

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“…G. parasuis can be colonized in the upper respiratory tract of  * Corresponding author: yrh2018@syau.edu.cn (Rong H. Yin)  # Yong C. Jia and Xin Chen contributed equally to this work. healthy porcine [2]. When the bacterium causes disease, some typical symptoms appear such as polyserositis, meningitis and arthritis.…”

Section: Introductionmentioning

confidence: 99%

“…Glaesserella parasuis , the etiological agent of Glässer’s disease, is one of the important bacteria affecting the pathogenesis of pigs [ 21 ]. G. parasuis can be colonized in the upper respiratory tract of healthy porcine [ 2 ]. When the bacterium causes disease, some typical symptoms appear such as polyserositis, meningitis and arthritis.…”

mentioning

confidence: 99%

Application of mouse model for evaluation of recombinant LpxC and GmhA as novel antigenic vaccine candidates of <i>Glaesserella parasuis</i> serotype 13

Jia

Chen

Zhou

et al. 2021

The Journal of Veterinary Medical Science

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Glaesserella parasuis (G. parasuis) has been one of the bacteria affecting the large-scale swine industry. Lack of an effective vaccine has limited control of the disease, which has an effect on prevalence. In order to improve the cross-protection of vaccines, development on subunit vaccines has become a hot spot. In this study, we firstly cloned the lpxC and gmhA genes from G. parasuis serotype 13 isolates, and expressed and purified their proteins. The results showed that LpxC and GmhA can stimulate mice to produce IgG antibodies. Through testing the cytokine levels of interleukin 4 (IL-4), IL-10 and interferon-γ (IFN-γ), it is found that recombinant GmhA, the mixed LpxC and GmhA can stimulate the body to produce Th1 and Th2 immune responses, while recombinant LpxC and inactivated bacteria can only produce Th2 immune responses. On the protection rate for mice, recombinant LpxC, GmhA and the mixture of LpxC and GmhA can provide 50%, 50% and 60% protection for lethal dose of G. parasuis infection, respectively. The partial protection achieved by the recombinant LpxC and GmhA supports their potential as novel vaccine candidate antigens against G. parasuis.

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Immunogenic characterization of vaccines based on Haemophilus parasuis Nagasaki strain, OmpP2, OmpP5 and OmpD15, in colostrum-deprived pigs experimentally challenged with the same strain

Cited by 9 publications

References 25 publications

Assessment of the Macrophage Scavenger Receptor CD163 in Mediating Glaesserella parasuis Infection of Host Cells

Assessment of the Macrophage Scavenger Receptor CD163 in Mediating Glaesserella parasuis Infection of Host Cells

Screening of linear B-cell epitopes and its proinflammatory activities of Haemophilus parasuis outer membrane protein P2

Application of mouse model for evaluation of recombinant LpxC and GmhA as novel antigenic vaccine candidates of <i>Glaesserella parasuis</i> serotype 13

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