Immunogenic characterization and protective efficacy of recombinant CsgA, major subunit of curli fibers, against Vibrio parahaemolyticus

Karan, Sweta; Choudhury, Devapriya; Dixit, Aparna

doi:10.1007/s00253-020-11038-4

Cited by 12 publications

(8 citation statements)

References 54 publications

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“…The selected CsgA protein showed an antigenic score of 0.941160 at ANTIGENpro and 1.0694 at VaxiJen, which suggested that the protein is highly antigenic which is evident by some previous studies [81], [82]. Unipro UGENE showed the protein as highly conserved among the 30 selected strains ( Supplementary data 3 ).…”

Section: Resultssupporting

confidence: 53%

“…Therefore, based on the results, the extracellular CsgA should be a prospective candidate [95]. A previous study reported that Vibrio parahaemolyticus CsgA can produce robust immune response and protection against the V. parahaemolyticus in BALB/c mice [82]. Moreover, Salmonella CsgA is highly conserved in across many strains (Supplementary data 3 ), therefore, we selected CsgA as a vaccine candidate to develop pan- Salmonella vaccine.…”

Section: Discussionmentioning

confidence: 99%

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An Integrated Comparative Genomics, Subtractive Proteomics and Immunoinformatics Framework for the Rational Design of a Pan-SalmonellaMulti-Epitope Vaccine

Bhattacharjee,

Hosen,

Lamisa

et al. 2023

Preprint

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Salmonella infections are a global public health issue due to the high cost of illness surveillance, prevention, and treatment. In this study, we explored the core proteome in Salmonella to design a multi-epitope vaccine through Subtractive Proteomics and immunoinformatics approaches. A total of 2395 core proteins presents in 30 different strains of Salmonella (reference strain-NZ CP014051) were curated. Utilizing the subtractive proteomics approach on the Salmonella core proteome, Curlin major subunit A (CsgA) was selected as the vaccine candidate. csgA is a conserved gene that is related with biofilm formation. Immunodominant B and T cell epitopes from CsgA were predicted using numerous immunoinformatics tools. T lymphocyte epitopes had adequate population coverage and their corresponding MHC alleles showed significant binding scores after peptide-protein based molecular docking. Afterward, a multiepitope vaccine was constructed with peptide linkers and Human Beta Defensin-2 (as an adjuvant). The vaccine was found to be highly antigenic, non-toxic, non-allergic, and had physicochemical properties. Additionally, Molecular Dynamics Simulation and Immune Simulation demonstrated that the vaccine can bind with Toll Like Receptor 4 and elicit robust immune response. Using in vitro, in vivo, and clinical trials, our results would yield a Pan-Salmonella vaccine that will provide protection against various Salmonella species.

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Section: Resultssupporting

confidence: 53%

Section: Discussionmentioning

confidence: 99%

An Integrated Comparative Genomics, Subtractive Proteomics and Immunoinformatics Framework for the Rational Design of a Pan-SalmonellaMulti-Epitope Vaccine

Bhattacharjee,

Hosen,

Lamisa

et al. 2023

Preprint

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“…Afterward, outbreaks of human gastroenteritis caused by V. parahaemolyticus occurred in many Asian nations and consequently around the world (Elmahdi et al 2016 ; Meparambu Prabhakaran et al 2020 ). It was estimated that more than 50% of foodborne gastrointestinal-Vibriosis cases were caused by V. parahaemolyticus in the United States (Karan et al 2021 ), which led to 45,000 illnesses each year ( https://www.cdc.gov/Vibrio/ , accessed on 26 April 2021). Sporadic outbreaks have also been reported in coastal European nations (Meparambu Prabhakaran et al 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Prophage-encoded gene VpaChn25_0734 amplifies ecological persistence of Vibrio parahaemolyticus CHN25

Yang

Wang

et al. 2022

Curr Genet

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Vibrio parahaemolyticus is a waterborne pathogen that can cause acute gastroenteritis, wound infection, and septicemia in humans. The molecular basis of its pathogenicity is not yet fully understood. Phages are found most abundantly in aquatic environments and play a critical role in horizontal gene transfer. Nevertheless, current literature on biological roles of prophage-encoded genes remaining in V. parahaemolyticus is rare. In this study, we characterized one such gene VpaChn25_0734 (543-bp) in V. parahaemolyticus CHN25 genome. A deletion mutant ΔVpaChn25_0734 (543-bp) was obtained by homologous recombination, and a revertant ΔVpaChn25_0734-com (543-bp) was also constructed. The ΔVpaChn25_0734 (543-bp) mutant was defective in growth and swimming mobility particularly at lower temperatures and/or pH 7.0–8.5. Cell surface hydrophobicity and biofilm formation were significantly decreased in the ΔVpaChn25_0734 (543-bp) mutant (p < 0.05). Based on the in vitro Caco-2 cell model, the deletion of VpaChn25_0734 (543-bp) gene significantly reduced the cytotoxicity of V. parahaemolyticus CHN25 to human intestinal epithelial cells (p < 0.05). Comparative secretomic and transcriptomic analyses revealed a slightly increased extracellular proteins, and thirteen significantly changed metabolic pathways in the ΔVpaChn25_0734 (543-bp) mutant, showing down-regulated carbon source transport and utilization, biofilm formation, and type II secretion system (p < 0.05), consistent with the observed defective phenotypes. Taken, the prophage-encoded gene VpaChn25_0734 (543-bp) enhanced V. parahaemolyticus CHN25 fitness for survival in the environment and the host. The results in this study facilitate better understanding of pathogenesis and genome evolution of V. parahaemolyticus, the leading sea foodborne pathogen worldwide.

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“…The bending stiffness of CsgA nanofibers changes with their diameter. , Recombinant CsgA has also been used to study the immune and protective effects of Vibrio parahaemolyticus . NMR technology has also been applied to observe the secretion targeting of preamyloid aggregates and the Curli subunit CsgA .…”

Section: Introductionmentioning

confidence: 99%

“…25,45 Recombinant CsgA has also been used to study the immune and protective effects of Vibrio parahaemolyticus. 46 NMR technology has also been applied to observe the secretion targeting of preamyloid aggregates and the Curli subunit CsgA. 47 The bending stiffness of linear fibers, 48,49 as measured by atomic force microscopy, is different from the tensile stiffness.…”

Section: Introductionmentioning

confidence: 99%

Force Spectra of a Single CsgA Molecule and Amyloid Nanofibers Assembled from Chimeric Mfp5 and CBD Proteins: Implications for a Nanomaterial Testing Machine

Liu

et al. 2022

ACS Appl. Nano Mater.

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The CsgA protein in Escherichia coli can self-assemble into an amyloid nanofiber, making it a promising biomaterial capable of resisting harsh environments. The assembling and unfolding mechanism of a single CsgA molecule is essential for all applications of CsgA materials but remains poorly understood. CsgA-CBD and Mfp5-CsgA are chimeric proteins generated by introducing the chitin-binding domain (CBD) and mussel foot protein 5 (Mfp5) into CsgA. In this study, we used magnetic tweezers to study the mechanical properties of a single CsgA molecule and the CsgA-CBD and Mfp5-CsgA assembled nanofibers. Our results demonstrated that the unfolding spectra of five β-sheets of a single CsgA molecule were not uniform and that several β-sheets simultaneously broke after an external force was applied. We applied ∼331 kcal/mol of work, via mechanochemical coupling, to unfold CsgA to a 94.6% strain. The average persistence lengths of the CsgA-CBD and Mfp5-CsgA nanofibers were 2.7 and 4.5 nm, respectively, while Young’s moduli of the two nanofibers were 42.8 and 56.1 MPa, respectively, both of which were less than those of the CsgA nanofiber. A relatively high force was required to unfold some of the nanofibers, which indicated that mechanical stimuli could help remove amyloid particles. This study assesses the mechanical properties of a single CsgA and lays the foundation for its subsequent use as a biological material and provides new insights into mechanochemical coupling nanofibers to design materials with tunable functions.

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Immunogenic characterization and protective efficacy of recombinant CsgA, major subunit of curli fibers, against Vibrio parahaemolyticus

Cited by 12 publications

References 54 publications

An Integrated Comparative Genomics, Subtractive Proteomics and Immunoinformatics Framework for the Rational Design of a Pan-SalmonellaMulti-Epitope Vaccine

An Integrated Comparative Genomics, Subtractive Proteomics and Immunoinformatics Framework for the Rational Design of a Pan-SalmonellaMulti-Epitope Vaccine

Prophage-encoded gene VpaChn25_0734 amplifies ecological persistence of Vibrio parahaemolyticus CHN25

Force Spectra of a Single CsgA Molecule and Amyloid Nanofibers Assembled from Chimeric Mfp5 and CBD Proteins: Implications for a Nanomaterial Testing Machine

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