Immunogenic and protective antigens of Brucella as vaccine candidates

Jezi, Faramarz Masjedian; Razavi, Shabnam; Mirnejad, Reza; Zamani, Khosrow

doi:10.1016/j.cimid.2019.03.015

Cited by 22 publications

(8 citation statements)

References 103 publications

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“…Several antigenic components of Brucella such as Omp16, Omp19, Omp25, Omp31, SurA, Dnak, trigger factor (TF), ribosomal protein L7L12, bacterioferritin (BFR) P39, and lumazine synthase BLS have been characterized (Barrionuevo and Giambartolomei 2019;Bundle et al 1989;Yin et al 2019). Efforts were instead concentrated on surface antigens (described as R in rough strain and A, M in smooth strains) and lipopolysaccharide (LPS) expected to trigger specific antibody response (Głowacka et al 2018;Jezi et al 2019;Mancilla 2016). The specific structure of LPS in the Brucella confers resistance to antimicrobial drugs, while inducing a very low endotoxicity.…”

Section: Pathogenesis Of Brucella Spp and Involved Immune Mechanismsmentioning

confidence: 99%

Importance of brucellosis control programs of livestock on the improvement of one health

Dadar

Tiwari

Sharun

et al. 2021

Veterinary Quarterly

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Brucellosis not only represents an important health restraint on livestock but also causes high economic losses in many developing countries worldwide. Despite considerable efforts made for the control of brucellosis, the disease is still spreading in many regions (such as the Middle East) where it represents one of the most important health hazards impacting both animals and humans. The present review aims to investigate the efficacy of veterinary control programs regarding brucellosis, with a special focus on current prevention, control, and eradication approaches. The reasons for unsuccessful control programs such as the absence of highly effective vaccines and non-certified bulls are also debated, to understand why the prevalence of brucellosis in livestock is not decreasing in many areas despite considerable efforts taken to date. The importance of governmental and regional investment in brucellosis control remains one of the main limiting factors owing to the limited budget allocated to tackle this disease. In this context, one health concept has generated novel comprehensive approaches with multiple economic implications across the livestock industry and public health. However, the implementation of such global preventive strategies appears to be a key issue for many endemic and low-income countries. According to the collected data, epidemiological contexts including management and trade systems along with well-defined agro-ecological zones should be evaluated in brucellosis endemic countries to improve milk production and to enhance the sustainability of the livestock sector at both national and regional levels.

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Section: Pathogenesis Of Brucella Spp and Involved Immune Mechanismsmentioning

confidence: 99%

Importance of brucellosis control programs of livestock on the improvement of one health

Dadar

Tiwari

Sharun

et al. 2021

Veterinary Quarterly

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“…Subunit vaccines have promising applications with the advantage of being safe, cost-effective and efficacious. During the past two decades, various antigens have been extracted from Brucella , such as Omp19, Omp25, L7/L12, P39, SodC, InpB, AsnC and TF [ 7 – 16 ]. These available antigens have been shown to provide protection against Brucella infection by reducing the organ bacterial load in mice.…”

Section: Introductionmentioning

confidence: 99%

Screening of potential vaccine candidates against pathogenic Brucella spp. using compositive reverse vaccinology

Zai

Yin

Guo

et al. 2021

Vet Res

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Brucella spp. are Gram-negative, facultative intracellular bacteria that cause brucellosis in humans and various animals. The threat of brucellosis has increased, yet currently available live attenuated vaccines still have drawbacks. Therefore, subunit vaccines, produced using protein antigens and having the advantage of being safe, cost-effective and efficacious, are urgently needed. In this study, we used core proteome analysis and a compositive RV methodology to screen potential broad-spectrum antigens against 213 pathogenic strains of Brucella spp. with worldwide geographic distribution. Candidate proteins were scored according to six biological features: subcellular localization, antigen similarity, antigenicity, mature epitope density, virulence, and adhesion probability. In the RV analysis, a total 32 candidate antigens were picked out. Of these, three proteins were selected for assessment of immunogenicity and preliminary protection in a mouse model: outer membrane protein Omp19 (used as a positive control), type IV secretion system (T4SS) protein VirB8, and type I secretion system (T1SS) protein HlyD. These three antigens with a high degree of conservation could induce specific humoral and cellular immune responses. Omp19, VirB8 and HlyD could substantially reduce the organ bacterial load of B. abortus S19 in mice and provide varying degrees of protection. In this study, we demonstrated the effectiveness of this unique strategy for the screening of potential broad-spectrum antigens against Brucella. Further evaluation is needed to identify the levels of protection conferred by the vaccine antigens against wild-type pathogenic Brucella species challenge.

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“…At the present medical level, it is difficult to completely eliminate Brucella [ 7 ]. Therefore, the vaccine is an ideal way to prevent Brucella infection [ 8 ]. Currently, there are no Brucella vaccines for humans, and the live-attenuated vaccines designed for animals have many defects, including interference with serological testing and human infectivity [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Design of a new multi-epitope vaccine against Brucella based on T and B cell epitopes using bioinformatics methods

et al. 2021

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Brucellosis is one of the most serious and widespread zoonotic diseases, which seriously threatens human health and the national economy. This study was based on the T/B dominant epitopes of Brucella outer membrane protein 22 (Omp22), outer membrane protein 19 (Omp19) and outer membrane protein 28 (Omp28), with bioinformatics methods to design a safe and effective multi-epitope vaccine. The amino acid sequences of the proteins were found in the National Center for Biotechnology Information (NCBI) database, and the signal peptides were predicted by the SignaIP-5.0 server. The surface accessibility and hydrophilic regions of proteins were analysed with the ProtScale software and the tertiary structure model of the proteins predicted by I-TASSER software and labelled with the UCSF Chimera software. The software COBEpro, SVMTriP and BepiPred were used to predict B cell epitopes of the proteins. SYFPEITHI, RANKpep and IEDB were employed to predict T cell epitopes of the proteins. The T/B dominant epitopes of three proteins were combined with HEYGAALEREAG and GGGS linkers, and carriers sequences linked to the N-and C-terminus of the vaccine construct with the help of EAAAK linkers. Finally, the tertiary structure and physical and chemical properties of the multi-epitope vaccine construct were analysed. The allergenicity, antigenicity and solubility of the multi-epitope vaccine construct were 7.37-11.30, 0.788 and 0.866, respectively. The Ramachandran diagram of the mock vaccine construct showed 96.0% residues within the favoured and allowed range. Collectively, our results showed that this multi-epitope vaccine construct has a high-quality structure and suitable characteristics, which may provide a theoretical basis for future laboratory experiments.

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Immunogenic and protective antigens of Brucella as vaccine candidates

Cited by 22 publications

References 103 publications

Importance of brucellosis control programs of livestock on the improvement of one health

Importance of brucellosis control programs of livestock on the improvement of one health

Screening of potential vaccine candidates against pathogenic Brucella spp. using compositive reverse vaccinology

Design of a new multi-epitope vaccine against Brucella based on T and B cell epitopes using bioinformatics methods

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