Immunofluorescent staining of keratin fibers in cultured cells

Sun, Tung‐Tien; Green, Howard

doi:10.1016/0092-8674(78)90233-7

Cited by 342 publications

(143 citation statements)

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“…Previous studies, testing individual cytostatic drugs, have shown them to be relatively ineffective in altering the distribution of cytokeratin . Disruption of microfilaments with cytochalasins (10,17) or microtubules by colchicine derivatives (13,17) does not significantly alter the inherent organization of cytokeratins . However, a dramatic reorganization of cytokeratin filaments occurs in HeLa cells and other cultured epithelial cell types (17) after combined treatment of cells with cytochalasins, colchicine, and related compounds.…”

mentioning

confidence: 99%

“…Keratin filament organization has been shown to be remarkably stable in epithelial cells in vitro by immunological methods (10,13,14) . Microinjection of anti-a-keratin antibodies into epithelial cells has been used to directly alter the organization of the keratin cytoskeleton (11,12) .…”

mentioning

confidence: 99%

“…Lazarides (5) has suggested that they participate in the organization and mechanical integration of the cytoplasm . Attention has recently focused on elucidating the structure, function, and organization of keratins in epithelial cells, particularly their utilization as elements of an intermediate-filament cytoskeleton where they are designated`cytokeratins' (7)(8)(9)(10) .…”

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confidence: 99%

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Rearrangement of the keratin cytoskeleton after combined treatment with microtubule and microfilament inhibitors.

Knapp¹,

O’Guin²,

Sawyer³

1983

The Journal of Cell Biology

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In addition to containing microtubule and microfilament systems, vertebrate epithelial cells contain an elaborate keratin intermediate-filament cytoskeleton . Little is known about its structural organization or function . Using indirect immunofluorescence microscopy with an antikeratin antiserum probe, we found that destabilization of microtubules and microfilaments with cytostatic drugs induces significant alterations in the cytoskeletal organization of keratin filaments in HeLa and fetal mouse epidermal cells . Keratin filament organization was observed to undergo a rapid (1-2 h) transition from a uniform distribution to an open lattice of keratin fibers stabilized by membrane-associated focal centers . Since addition of any one drug alone did not elicit significant organizational change in the keratin cytoskeleton, we suggest that microfilaments and microtubules have a combined role in maintaining the arrangement of keratin in these cells . Vimentin filaments, the only other intermediatesized filaments found in HeLa cells, did not co-distribute with keratin in untreated or drugtreated cells . These findings offer a new way to approach the study of the dynamics and functional roles of the keratin cytoskeleton in epithelial cells .

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confidence: 99%

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Rearrangement of the keratin cytoskeleton after combined treatment with microtubule and microfilament inhibitors.

Knapp¹,

O’Guin²,

Sawyer³

1983

The Journal of Cell Biology

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“…The two most acidic proteins, b, c and h, i, from each of the two keratins were phosphorylated. Keratins from human epithelial cells in culture (Sun & Green, 1978 b) and from both human and rodent tissue are also known to be processed by phosphorylation (Franke et al, 1981;Sun & Green, 1978b).…”

Section: Discussionmentioning

confidence: 99%

“…The three fibrillar structures were identified by indirect immunofluorescence staining with either monoclonal or polyclonal, monospecific antibodies made against each of the proteins (Norrild et al, 1986;Sun & Green, 1978a;Weber & Osborn, 1981). Distinct functions are associated with the different cytoskeleton filaments.…”

Section: Introductionmentioning

confidence: 99%

Herpes Simplex Virus-induced Changes of the Keratin Type Intermediate Filament in Rat Epithelial Cells

Nielsen

Forchhammer²,

Dabelsteen

et al. 1987

Journal of General Virology

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SUMMARYHerpes simplex virus type 1 (HSV-1) infection of human fibroblast cells grown in culture induces reorganization of the cytoskeleton fibrillar structures. Normal transport and insertion of HSV glycoproteins into the plasma membrane of the cells depend on the integrity of the microtubules. The natural host cells for HSV are epithelial cells, and an epithelial cell line established from rat palate was used in the present study. The effect of virus on the structure of the intermediate filaments and especially on the keratin proteins was studied. Two-dimensional gel electrophoresis of total cell extracts identified in uninfected cells two major acidic keratin proteins with apparent molecular weights of 44000 (44K)

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Immunofluorescent and Immunoperoxidase Staining of Antibodies to Fibrous Keratin

Robinson¹

1984

Arch Dermatol

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Immunofluorescent staining of keratin fibers in cultured cells

Cited by 342 publications

References 38 publications

Rearrangement of the keratin cytoskeleton after combined treatment with microtubule and microfilament inhibitors.

Rearrangement of the keratin cytoskeleton after combined treatment with microtubule and microfilament inhibitors.

Herpes Simplex Virus-induced Changes of the Keratin Type Intermediate Filament in Rat Epithelial Cells

Immunofluorescent and Immunoperoxidase Staining of Antibodies to Fibrous Keratin

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