Immunofluorescent localization of ubiquitin and proteasomes in nucleolar vacuoles of soybean root meristematic cells

Stępiński, Dariusz

doi:10.4081/ejh.2012.13

Cited by 10 publications

(6 citation statements)

References 61 publications

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“…It has been suggested that the ubiquitin-proteasome system may be active in the nucleolus, where ubiquitin and proteasome were immunolocalized (Stępi nski, 2012). In addition, this compartment provides a site for the transient inactivation of enzymatic or regulatory proteins (Stępi nski, 2014).…”

Section: Resultsmentioning

confidence: 99%

Manipulation of a Senescence-Associated Gene Improves Fleshy Fruit Yield

et al. 2017

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Senescence is the process that marks the end of a leaf's lifespan. As it progresses, the massive macromolecular catabolism dismantles the chloroplasts and, consequently, decreases the photosynthetic capacity of these organs. Thus, senescence manipulation is a strategy to improve plant yield by extending the leaf's photosynthetically active window of time. However, it remains to be addressed if this approach can improve fleshy fruit production and nutritional quality. One way to delay senescence initiation is by regulating key transcription factors (TFs) involved in triggering this process, such as the NAC TF ORESARA1 (ORE1). Here, three senescence-related NAC TFs from tomato (Solanum lycopersicum) were identified, namely SlORE1S02, SlORE1S03, and SlORE1S06. All three genes were shown to be responsive to senescence-inducing stimuli and posttranscriptionally regulated by the microRNA miR164. Moreover, the encoded proteins interacted physically with the chloroplast maintenance-related TF SlGLKs. This characterization led to the selection of a putative tomato ORE1 as target gene for RNA interference knockdown. Transgenic lines showed delayed senescence and enhanced carbon assimilation that, ultimately, increased the number of fruits and their total soluble solid content. Additionally, the fruit nutraceutical composition was enhanced. In conclusion, these data provide robust evidence that the manipulation of leaf senescence is an effective strategy for yield improvement in fleshy fruit-bearing species.

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Section: Resultsmentioning

confidence: 99%

Manipulation of a Senescence-Associated Gene Improves Fleshy Fruit Yield

et al. 2017

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“…Plant nucleoli also usually contain so called nucleolar cavities or vacuoles (NoV) often located in the central part of the nucleolus (Figure 1 ; Brown and Shaw, 1998 ; Shaw and Brown, 2004 ). Although the function of the NoVs is currently unknown, it could be suggested that these structures may be regions of temporal sequestration and storage of some biochemical factors such as elements of the ubiquitin-proteasome system (Stepinski, 2012 ), snoRNAs (snoRNPs) and spliceosomal small nuclear RNAs (snRNAs/snRNPs) (Beven et al, 1996 ; Lorković and Barta, 2008 ), which may be released into the nucleoplasm depending on specific physiological requirements incurred during stress responses or at particular developmental stages (Mineur et al, 1998 ).…”

Section: Plant Nucleolar Organizationmentioning

confidence: 99%

The Multiple Functions of the Nucleolus in Plant Development, Disease and Stress Responses

et al. 2018

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The nucleolus is the most conspicuous domain in the eukaryotic cell nucleus, whose main function is ribosomal RNA (rRNA) synthesis and ribosome biogenesis. However, there is growing evidence that the nucleolus is also implicated in many other aspects of cell biology, such as regulation of cell cycle, growth and development, senescence, telomerase activity, gene silencing, responses to biotic and abiotic stresses. In the first part of the review, we briefly assess the traditional roles of the plant nucleolus in rRNA synthesis and ribosome biogenesis as well as possible functions in other RNA regulatory pathways such as splicing, nonsense-mediated mRNA decay and RNA silencing. In the second part of the review we summarize recent progress and discuss already known and new hypothetical roles of the nucleolus in plant growth and development. In addition, this part will highlight studies showing new nucleolar functions involved in responses to pathogen attack and abiotic stress. Cross-talk between the nucleolus and Cajal bodies is also discussed in the context of their association with poly(ADP ribose)polymerase (PARP), which is known to play a crucial role in various physiological processes including growth, development and responses to biotic and abiotic stresses.

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“…30,32,94,96 In this perspective, it becomes primarily important to discriminate cells in the different phases of proliferation, differentiation, aging and death, through specific molecular markers: multiple immunocytochemical techniques were widely applied to this aim. 24,31,38,43,45,97–99 A manuscript only was devoted to plant cell development, 100 and this confirms that histochemistry is still relatively poorly used by plant biologists in their research: actually, in the last three years, only 30 papers have been published, in which histochemical techniques have been used in investigations on plant cell biology (source: www.scopus.com). In recent years, connective tissues and, in particular, bone and cartilage have become increasingly popular subjects for the authors of the European Journal of Histochemistry .…”

Section: A Survey Of the Recent Contents Of A Long-time Established Hmentioning

confidence: 85%

On the future contents of a small journal of histochemistry

Pellicciari

2012

Eur J Histochem

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In the last three years, more than 70,000 scientific articles have been published in peer reviewed journals on the application of histochemistry in the biomedical field: most of them did not appear in strictly histochemical journals, but in others dealing with cell and molecular biology, medicine or biotechnology. This proves that histochemistry is still an active and innovative discipline with relevance in basic and applied biological research, but also demonstrates that especially the small histochemical journals should likely reconsider their scopes and strategies to preserve their authorship. A review of the last three years volumes of the European Journal of Histochemistry, taken as an example of a long-time established small journal, confirmed that the published articles were widely heterogeneous in their topics and experimental models, as in this journal's tradition. This strongly suggests that a journal of histochemistry should keep its role as a forum open to an audience as broad as possible, publishing papers on cell and tissue biology in a wide variety of models. This will improve knowledge of the basic mechanisms of development and differentiation, while helping to increase the number of potential authors since scientists who generally do not use histochemistry in their research will find hints for the applications of histochemical techniques to novel still unexplored subjects.

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Immunofluorescent localization of ubiquitin and proteasomes in nucleolar vacuoles of soybean root meristematic cells

Cited by 10 publications

References 61 publications

Manipulation of a Senescence-Associated Gene Improves Fleshy Fruit Yield

Manipulation of a Senescence-Associated Gene Improves Fleshy Fruit Yield

The Multiple Functions of the Nucleolus in Plant Development, Disease and Stress Responses

On the future contents of a small journal of histochemistry

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