Immunofluorescent localisation of human pregnancy-specific β-globulin in placenta and chorioepithelioma

Tatarinov, Yu. S.; Falaleeva, D. M.; Kalashnikov, V. V.; Toloknov, B. O.

doi:10.1038/260263a0

Cited by 67 publications

(24 citation statements)

References 9 publications

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“…Recent immunofluorescence studies of human placenta suggest that cytotrophoblast cells synthesize some of the pregnancy-specific fll-glycoprotein found in maternal serum (48). Whether cytotrophoblast contributes to the production of HCT, a glycoprotein hormone whose site of production in the placenta has not been localized (26,27), is unknown.…”

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confidence: 99%

Cytological events involved in glycoprotein synthesis in cellular and syncytial trophoblast of human placenta. An electron microscope autoradiographic study of [3H]galactose incorporation.

Nelson

Enders

King

1978

The Journal of Cell Biology

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Electron microscope autoradiography was used to study glycoprotein synthesis in cellular trophoblast (cytotrophoblast) and syncytial trophoblast of term human placental villi incubated in vitro with D-[1-aH]galactose ([SH]gal). Autoradiographs were analyzed using the hypothetical grain analysis of Blackett and Parry (1973. J. Cell Biol. 57:9-15 [aH]gal incorporated into cytotrophoblast during the pulse incubation remained intracellular through the duration of the experiment. There was little autoradiographic evidence for secretion of tritiated macromolecules. Cytotrophoblast incubated for the longest time period studied (4 h +) showed a substantial concentration of tritiated macromolecules in the Golgi complex and in the ground plasm but not in the rough endoplasmic reticulum.KEY WORDS placenta glycoproteins electron microscopy trophoblast 9 autoradiography Tritiated monosaccharides have been used in a number of studies to label glycoproteins whose synthesis and secretion are to be followed by light and/or electron microscope autoradiography. These studies have demonstrated that some glycoproteins that are synthesized remain within the cell (5,6,15,37,38,43) while others are added either to the surface coat (3,4,6,23,28,29,41) or to the basal lamina (12, 13). In other cases, the glycoprotein synthesized is destined for secre- 418

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confidence: 99%

Cytological events involved in glycoprotein synthesis in cellular and syncytial trophoblast of human placenta. An electron microscope autoradiographic study of [3H]galactose incorporation.

Nelson

Enders

King

1978

The Journal of Cell Biology

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“…Recently the occurrence of a high molecular weight variant was proposed [25]. The protein is synthesized in the syncytiotrophoblast of the placenta [8,18] and secreted into the maternal blood in increasing concentrations äs pregnancy proceeds [5,6,16,21,29,31]. Its detection is possible äs early äs 18 days W ra et al, SPi in normal and pathologic pregnancies l Materials and methods 1.1 Blood samples were taken from healthy pr gnant women and from hospitalized and outpatients.…”

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confidence: 99%

Radioimmunoassay of SP₁(Pregnancy-specificβ₁-glycoprotein) in maternal blood and in amniotic fluid in normal and pathologic pregnancies

Würz¹,

Geiger²,

Künzig³

et al. 1981

Journal of Perinatal Medicine

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“…PREGNANCY-SPECIFIC 1i-glycoprotein (SP1) (Bohn, 1971) has been localized in the human placental syncytiotrophoblast (Horne et al, 1976;Tatarinov et al, 1976). It is secreted into the maternal circulation early in pregnancy (Grudzinskas et al, 1977) and the levels increase toward term.…”

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confidence: 99%

“…It is secreted into the maternal circulation early in pregnancy (Grudzinskas et al, 1977) and the levels increase toward term. In addition to normal placenta, choriocarcinoma tissue contains SPI (Tatarinov et al, 1976). Recently it was found that some human fibroblast strains and an ovarian cystadenocarcinoma cell line elaborate SPI in vitro (Rosen et al, 1979;Azer et al, 1980).…”

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confidence: 99%

Cells from normal brain and gliomas synthesize pregnancy-specific β1-glycoprotein-like material in vitro

Heikinheimo¹,

Paasivuo²,

Wahlström³

1981

Br J Cancer

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Summary.-The synthesis of pregnancy-specific fl-glycoprotein (SP1) was studied in normal brain-derived and malignant glial-cell cultures. The normal brainderived and glioma cells were found to contain SP1 when studied by radioimmunoassay and by the triple-bridge immunoperoxidase technique. The active synthesis of SP1 by these brain-derived cells was confirmed by metabolic labelling and subsequent immune precipitation of the culture medium. The SPl -like material produced by the brain-derived cells had the same molecular weight as purified placental SP1.

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Immunofluorescent localisation of human pregnancy-specific β-globulin in placenta and chorioepithelioma

Cited by 67 publications

References 9 publications

Cytological events involved in glycoprotein synthesis in cellular and syncytial trophoblast of human placenta. An electron microscope autoradiographic study of [3H]galactose incorporation.

Cytological events involved in glycoprotein synthesis in cellular and syncytial trophoblast of human placenta. An electron microscope autoradiographic study of [3H]galactose incorporation.

Radioimmunoassay of SP₁(Pregnancy-specificβ₁-glycoprotein) in maternal blood and in amniotic fluid in normal and pathologic pregnancies

Cells from normal brain and gliomas synthesize pregnancy-specific β1-glycoprotein-like material in vitro

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Immunofluorescent localisation of human pregnancy-specific β-globulin in placenta and chorioepithelioma

Cited by 67 publications

References 9 publications

Cytological events involved in glycoprotein synthesis in cellular and syncytial trophoblast of human placenta. An electron microscope autoradiographic study of [3H]galactose incorporation.

Cytological events involved in glycoprotein synthesis in cellular and syncytial trophoblast of human placenta. An electron microscope autoradiographic study of [3H]galactose incorporation.

Radioimmunoassay of SP1(Pregnancy-specificβ1-glycoprotein) in maternal blood and in amniotic fluid in normal and pathologic pregnancies

Cells from normal brain and gliomas synthesize pregnancy-specific β1-glycoprotein-like material in vitro

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Product

Resources

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Radioimmunoassay of SP₁(Pregnancy-specificβ₁-glycoprotein) in maternal blood and in amniotic fluid in normal and pathologic pregnancies