Immunofluorescent labeling of cancer marker Her2 and other cellular targets with semiconductor quantum dots

Wu, Xinfeng; Liu, Hongjian; Liu, Jianquan; Haley, Kari N.; Treadway, Joseph A.; Larson, Jeff; Ge, Nianfeng; Peale, Frank; Bruchez, Marcel P.

doi:10.1038/nbt764

Cited by 2,431 publications

(1,937 citation statements)

References 22 publications

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“…The DHLA-PEG ligand, however, afforded aqueous solutions of QDs which did not aggregate or bind protein in serum, giving a total HD of 8.7 nm ( Figure 1D). This is much smaller than the sizes reported for other QDs used in vivo, which range from about 15 to 30 nm in buffer, and include ligand spheres composed of polymers, 6 proteins, 7 cross-linked structures, 8 or multilayered combinations of ligands. 9 Any additional or non-specific protein binding, generally not well characterized in the literature, would further increase the effective sizes of these QD probes in vivo.…”

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confidence: 73%

Size Series of Small Indium Arsenide−Zinc Selenide Core−Shell Nanocrystals and Their Application to In Vivo Imaging

et al. 2006

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We have developed a size series of unusually small, water-soluble (InAs)ZnSe (core)shell quantum dots (QDs) that emit in the near infrared and exhibit new behavior in vivo, including multiple sequential lymph node mapping and extravasation from the vasculature. The biological utility of these fluorescent probes resulted from our intentional choice to match the semiconductor material and water soluble ligand with a desired final hydrodynamic diameter and emission wavelength.Semiconductor nanocrystals (or quantum dots, QDs) are excellent fluorophores due to their continuous absorption profiles at wavelengths to the blue of the band edge, high photostability, and narrow, tunable emission peaks. For in vivo biological imaging applications, the QD emission wavelength should ideally be in a region of the spectrum where blood and tissue absorb minimally but detectors are still efficient, approximately 700-900 nm in the near infrared (NIR). 1 In addition, the hydrodynamic size of the QD should be appropriately matched to the biological experiment of interest. 2 In previous work, for example, we described the efficacy of Type II QDs with hydrodynamic diameters (HD) of 15.8-18.8 nm to map sentinel lymph nodes selectively. 2a Here we report the synthesis of a size series of (InAs)ZnSe (core) shell QDs that emit in the near infrared and exhibit HD < 10 nm. We demonstrate their utility E-mail: mgb@mit.ed. Supporting Information Available: Experimental procedures, transmission electron microscopy data, and additional optical characterization, including emission stability in serum. in vivo by imaging multiple, sequential lymph nodes and showing extravasation from the vasculature in rat models, neither of which has been achieved before with QDs to our knowledge. NIH Public AccessWhile InAs QDs are known, most studies report emission wavelengths longer than 800 nm. 3 Until now, only Battaglia and Peng have shown well defined InAs first absorptioin peaks at wavelengths below 800 nm. 4 Their work, however, primarily concerned InP QDs. We have developed a procedure for the synthesis of a well-characterized size series of small InAs cores (diameters < 2 nm). Moreover we have extended the work to show the overcoating of these very small cores with a second, higher bandgap semiconductor shell. Zinc selenide was chosen as the ideal shell material due to its reasonably small lattice mismatch with zinc blende InAs (6.44%), its high bulk band offsets (1.26 and 0.99 eV for the conduction and valence bands, CB and VB, respectively), and its reported ability to increase the quantum yield (QY) of InAs cores by more than an order of magnitude. 3e Longer emission wavelengths, particularly the biologically desirable 800-840 nm range, can be achieved by (1) increasing the core size or (2) the shell thickness, or (3) by altering the band offsets between core and shell such as by adding a small amount of Cd to the ZnSe shell. Therefore, by varying the core size, and the shell thickness or composition, a wide tunability of the final emissi...

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confidence: 73%

Size Series of Small Indium Arsenide−Zinc Selenide Core−Shell Nanocrystals and Their Application to In Vivo Imaging

et al. 2006

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“…To confirm whether optical properties of QDs are more robust than organic probes (Wu et al, 2003) and may, therefore, better facilitate long-term live imaging and tracking of mammalian cells in vivo, we measured the variation of fluorescence intensity over time in viable N2a cells cotransfected with 620-nm QDs and farnesylated enhanced green fluorescence protein (EGFP-F) plasmid. While 2 min of repetitive scanning bleached the membrane-bound EGFP, the QD emission strikingly persisted throughout the experiment, despite that the peak irradiance for the QD excitation line was higher (560 mW/ mm 2 ) than the irradiance for the EGFP line (368 mW/mm 2 ; Fig.…”

Section: Properties Of Quantum Dotsmentioning

confidence: 99%

In vivo quantum dot labeling of mammalian stem and progenitor cells

Slotkin

Chakrabarti

Dai

et al. 2007

Developmental Dynamics

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“…Optical imaging has been used from diagnosing cancers in its earlier stages [746] to immunostainings, [747] fluorescence-guided surgery, and endoscopic imaging. [748] This technique is based on the detection of light, absorbed and emitted by fluorochrome concentrations as a source of contrast.…”

Section: Optical Imagingmentioning

confidence: 99%

Synthesis, Functionalization, and Design of Magnetic Nanoparticles for Theranostic Applications

Mosayebi

Kiyasatfar

Laurent

2017

Adv Healthcare Materials

193

171

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In order to translate nanotechnology into medical practice, magnetic nanoparticles (MNPs) have been presented as a class of non‐invasive nanomaterials for numerous biomedical applications. In particular, MNPs have opened a door for simultaneous diagnosis and brisk treatment of diseases in the form of theranostic agents. This review highlights the recent advances in preparation and utilization of MNPs from the synthesis and functionalization steps to the final design consideration in evading the body immune system for therapeutic and diagnostic applications with addressing the most recent examples of the literature in each section. This study provides a conceptual framework of a wide range of synthetic routes classified mainly as wet chemistry, state‐of‐the‐art microfluidic reactors, and biogenic routes, along with the most popular coating materials to stabilize resultant MNPs. Additionally, key aspects of prolonging the half‐life of MNPs via overcoming the sequential biological barriers are covered through unraveling the biophysical interactions at the bio–nano interface and giving a set of criteria to efficiently modulate MNPs' physicochemical properties. Furthermore, concepts of passive and active targeting for successful cell internalization, by respectively exploiting the unique properties of cancers and novel targeting ligands are described in detail. Finally, this study extensively covers the recent developments in magnetic drug targeting and hyperthermia as therapeutic applications of MNPs. In addition, multi‐modal imaging via fusion of magnetic resonance imaging, and also innovative magnetic particle imaging with other imaging techniques for early diagnosis of diseases are extensively provided.

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Immunofluorescent labeling of cancer marker Her2 and other cellular targets with semiconductor quantum dots

Cited by 2,431 publications

References 22 publications

Size Series of Small Indium Arsenide−Zinc Selenide Core−Shell Nanocrystals and Their Application to In Vivo Imaging

Size Series of Small Indium Arsenide−Zinc Selenide Core−Shell Nanocrystals and Their Application to In Vivo Imaging

In vivo quantum dot labeling of mammalian stem and progenitor cells

Synthesis, Functionalization, and Design of Magnetic Nanoparticles for Theranostic Applications

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