Immunofluorescence staining of phosphoinositides in primary mouse hippocampal neurons in dissociated culture

Guo, Zhenzhen; Tong, Chunfang; Yang, Yanrui; Li, Jiajia

doi:10.1016/j.xpro.2022.101549

STAR Protocols

2022

DOI: 10.1016/j.xpro.2022.101549

|View full text |Cite

Immunofluorescence staining of phosphoinositides in primary mouse hippocampal neurons in dissociated culture

Zhenzhen Guo

Chunfang Tong

Yanrui Yang

et al.

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2022

Publication Types

Select...

Article1

Relationship

Self Cite0

Independent1

Authors

Journals

Cited by 1 publication

(1 citation statement)

References 6 publications

(18 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…After washing with PBS, DAPI (4,6-diamino-2-phenylindole) (C1005, Beyotime, China) was used to dye the nucleus, then observed under microscope (LSM900, ZEISS, Germany) and captured pho-tographs on computer. For the detailed process, please refer to the research of Guo et al [43]. difference was regarded as significant when P < 0:05.…”

mentioning

confidence: 99%

Dietary Leucine Supplementation Improves Muscle Fiber Growth and Development by Activating AMPK/Sirt1 Pathway in Blunt Snout Bream (Megalobrama amblycephala)

Wang

Guo

Huang

et al. 2022

Aquaculture Nutrition

View full text Add to dashboard Cite

This research is aimed at evaluating the effects of leucine supplementation on muscle fibers growth and development of blunt snout bream through a feeding trial and a primary muscle cells treatment. An 8-week trial with diets containing 1.61% leucine (LL) or 2.15% leucine (HL) was conducted in blunt snout bream ( mean initial weight = 56.56 ± 0.83 g ). Results demonstrated that the specific gain rate and the condition factor of fish in the HL group were the highest. The essential amino acids content of fish fed HL diets was significantly higher than that fed LL diets. The texture (hardness, springiness, resilience, and chewiness), the small-sized fiber ratio, fibers density, and sarcomere lengths in fish all obtained the highest in the HL group. Additionally, the proteins expression related with the activation of the AMPK pathway (p-Ampk, Ampk, p-Ampk/Ampk, and Sirt1) and the expression of genes (myogenin (myog), myogenic regulatory factor 4 (mrf4) and myoblast determination protein (myod), and protein (Pax7) related to muscle fiber formation were significantly upregulated with increasing level of dietary leucine. In vitro, the muscle cells were treated with 0, 40 and 160 mg/L leucine for 24 h. The results showed that treated with 40 mg/L leucine significantly raised the protein expressions of BCKDHA, Ampk, p-Ampk, p-Ampk/Ampk, Sirt1, and Pax7 and the gene expressions of myog, mrf4, and myogenic factor 5 (myf5) in muscle cells. In summary, leucine supplementation promoted muscle fibers growth and development, which may be related to the activation of BCKDH and AMPK.

show abstract

mentioning

confidence: 99%

Dietary Leucine Supplementation Improves Muscle Fiber Growth and Development by Activating AMPK/Sirt1 Pathway in Blunt Snout Bream (Megalobrama amblycephala)

Wang

Guo

Huang

et al. 2022

Aquaculture Nutrition

View full text Add to dashboard Cite

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

hi@scite.ai

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Immunofluorescence staining of phosphoinositides in primary mouse hippocampal neurons in dissociated culture

Cited by 1 publication

References 6 publications

Dietary Leucine Supplementation Improves Muscle Fiber Growth and Development by Activating AMPK/Sirt1 Pathway in Blunt Snout Bream (Megalobrama amblycephala)

Dietary Leucine Supplementation Improves Muscle Fiber Growth and Development by Activating AMPK/Sirt1 Pathway in Blunt Snout Bream (Megalobrama amblycephala)

Contact Info

Product

Resources

About