Immunofluorescence pattern of antinuclear antibody and its association with autoantibody profile in systemic lupus erythematosus

Sharmin, Sadia; Ahmed, Sharmeen; Sattar, Humayun; Miah, Md. Ruhul Amin; Chowdhury, M R; Hassan, M. Masudul

doi:10.3329/bsmmuj.v6i2.29130

Cited by 3 publications

(4 citation statements)

References 11 publications

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“…In a previous Bangladeshi study also observed parallel result to the current study. [24] But in a Egyptian study, frequency of Anti ENA antibodies were found almost similar to present study except anti-RNP which was 10% in their study. [11] In our study, anti-RNP was more commonly associated with constitutional features including arthritis/arthralgia, malar rash, non-specific rash, photosensitivity and proteinuria.…”

Section: Discussionsupporting

confidence: 78%

Autoantibody Profile and Its Association with Clinical Presentation of Paediatric SLE Patients

Gomes¹,

Islam²,

Rahman³

2019

AJP

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Systemic lupus erythematosus (SLE) is a chronic autoimmune disease characterized by the production of various auto antibodies including anti-nuclear antibody, double stranded DNA and antibodies to extractable nuclear antigens (ENA), which include four groups of RNA binding protein namely Sm, RNP, SSA/Ro and SSB/La. The objectives were to detect the frequency and pattern of ANA, frequency of anti-ENA antibodies and association between the clinical presentations with ANA and Anti-ENA antibodies in paediatric SLE patients. It was a cross sectional study and the data was collected from patients fulfilling the ACR 1997 revised criteria of SLE in the Department of Paediatrics, BSMMU, Dhaka, Bangladesh. Among 50 paediatric SLE patients, ANA was positive in 94% of the patients. Homogenous pattern of ANA was the highest (57%) followed by speckled (31%), cytoplasmic (6.6%) and nucleolar (3.3%) patterns. Anti-ds DNA positivity was 86% and anti-ENA positivity was 56% in this study. Among them, anti-RNP was found in 43.5% followed by anti-sm (24%), anti-SSA (24%) and anti-SSB (17%). Anti-RNP antibody was associated with renal and gastrointestinal and Anti-SSA was associated with only gastrointestinal manifestations. ANA, anti ds-DNA, anti ENA positivity were found significantly among paediatric SLE patients and clinical presentation of them had association with Anti-ENA antibodies.

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Section: Discussionsupporting

confidence: 78%

Autoantibody Profile and Its Association with Clinical Presentation of Paediatric SLE Patients

Gomes¹,

Islam²,

Rahman³

2019

AJP

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“…Sharmin et al reported a sensitivity of 86.5% and Bentow et al reported a sensitivity of 81.5%. However, our results were higher than the reported 59% sensitivity of Karumanchi and Oommen [17][18][19].…”

Section: Discussioncontrasting

confidence: 95%

Comparison of Indirect Immunofluorescence and Enzyme Immunoassay for the Detection of Antinuclear Antibodies

Khalifah¹,

Almansouri²,

Mowallad³

et al. 2022

Cureus

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Objective: The detection of autoantibodies directed toward nuclear antigens is one of the main criteria for the diagnosis of systemic lupus erythematosus (SLE), for which the most commonly used techniques are the enzyme immunoassay and immunofluorescence assay (IFA). However, the sensitivity and specificity of these tests vary between different techniques. Thus, in this study, we aimed to determine the superior method for detecting antinuclear antibodies (ANAs) and compare the accuracy of tests ordered by rheumatologists versus non-rheumatologists.Materials and methods: We compared the sensitivity and specificity of the two assays in 149 patients from a non-selected population, who were sent to the immunology laboratory of King Abdulaziz Medical City, Jeddah from 2018 to 2019.Results: The sensitivity and specificity of the indirect IFA were 77.78 % and 58.65%, respectively. The positive and negative predictive values of IFA for SLE were 44.87% and 85.92%, respectively. The sensitivity and specificity of the enzyme-linked immunosorbent assay (ELISA) were 77.78% and 80.77%, respectively. The negative and positive predictive values of ELISA for SLE were 63.64% and 89.36%, respectively. The highest number of false-positive IFA tests was requested by family physicians and the lowest was requested by rheumatologists. Conclusion: Our data show that IFA has a higher negative predictive value, while ELISA has a higher positive predictive value. The positive predictive value of the test can be improved by pre-selecting patients by specialist rheumatologists.

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“…Speckled pattern (43.7%) followed by peripheral (34.3%) and homogenous pattern (21.8%) were present in their study. 15 Gonzalez and Rothdfield reported that the homogenous and peripheral patterns were found most frequently in SLE patients and only a few had speckled pattern. 23 Present study found homogenous (46.3%), speckled (17.6%), nucleolar (1.1%) and cytoplasmic (2.6%) staining pattern.Most of the literature reportedpresence of homogenous pattern in the majority of SLE cases.…”

Section: Resultsmentioning

confidence: 97%

“…7 Positive autoantibody titers at a dilution equal to or greater than 1:160 were usually considered as clinically significant. [15][16][17] Statistical analysis was done to assess the frequency of positivity, association of different patterns with different systemic involvement.…”

Section: Methodsmentioning

confidence: 99%

Pattern and Frequency of Anti-nuclear Antibody Positivity in Paediatric Rheumatic Diseases

Islam

Laila

Rahman

2019

Bangladesh J Child Health

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Background: Anti-nuclear antibodies (ANAs) are specific antibodies directed against a variety of nuclear antigens detected in the serum of patients with many rheumatic and non-rheumatic diseases.These antibodies are not only involved in the pathogenesis, but also constitute the basis for diagnosis and treatment of paediatric rheumatic diseases. The objective of the study was to identify the patterns and frequency of ANA positivity in Paediatric Rheumatic Diseases. Methodology: It was a retrospective study. Fourteen hundred and sixty eight records of paediaric rheumatology patients were analyzed. Statistical analysis were done to observe the frequency and association of different patterns of ANA in Juvenile idiopathic arthritis and systemic lupus erythematosus patients. Results: Among the 1468 patients of PRDs, frequency of JIA cases was the highest (65 %) followed by SLE, Scleroderma, juvenile dermatomyositis, and others. Among the 261 PRD patients ANA positivity was 65%. ANA positivity was 100%, 92%, 40% and 31.5% in Mixed connective tissue disease, SLE, JDM and Scleroderma patients respectively. Homogenous staining pattern was found in 59% and speckled pattern in 22.9%. There was significant association between ANA positivity and uveitis in oligoarticular JIA patients. Significant association was also found between homogeneous patterns of ANA and renal involvement in SLE patients. Conclusion: ANA positivity was highest in MCTD cases followed by SLE cases. Majority of SLE cases had homogeneous pattern of ANA.Staining patterns of ANA had significant association with the clinical manifestations in SLE and JIA cases. Bangladesh J Child Health 2019; VOL 43 (1) :21-26

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Immunofluorescence pattern of antinuclear antibody and its association with autoantibody profile in systemic lupus erythematosus

Abstract: Abstract:Background: Antinuclear antibody (ANA) is useful in the diagnosis of systemic lupus erythematosus (SLE). Association

Cited by 3 publications

References 11 publications

Autoantibody Profile and Its Association with Clinical Presentation of Paediatric SLE Patients

Autoantibody Profile and Its Association with Clinical Presentation of Paediatric SLE Patients

Comparison of Indirect Immunofluorescence and Enzyme Immunoassay for the Detection of Antinuclear Antibodies

Pattern and Frequency of Anti-nuclear Antibody Positivity in Paediatric Rheumatic Diseases

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